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Automated Platform for the Analysis of Multi-Plate Growth and Reporter Data.

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Titel: Automated Platform for the Analysis of Multi-Plate Growth and Reporter Data.
Autoren: Nahami, Avichay, Kain, Dor, Cohen, Yonatan, Kolodkin-Gal, Yuval, Assouline, Yohanan, Yona, Avihu H., Kolodkin-Gal, Ilana, Dorfan, Yuval
Quelle: Microorganisms; Aug2025, Vol. 13 Issue 8, p1889, 13p
Schlagwörter: GENE expression, USER interfaces, DENSITOMETRY, MICROBIAL sensitivity tests, DATA visualization software, DIGITAL technology, OPACITY (Optics)
Abstract: Researchers traditionally calculate growth rates using the natural logarithm of optical density (OD), with existing script packages facilitating this process. Automatic plate readers, capable of simultaneously measuring OD across 384 cultures, significantly enhance data collection efficiency. Furthermore, these readers also measure luminescence and fluorescence, providing valuable insights into gene expression. However, current analysis software often struggle with data generated by robotic systems measuring multiple plates, limiting the integration of growth and reporter analyses. This method paper addresses three key challenges: (a) the incompatibility of robotic multi-plate systems with existing analysis software, (b) the integration of growth and reporter analyses, and (c) the development of user-friendly interfaces for non-programmers. To address these challenges, we offer optimized script packages and a relevant case study on matrix expression in response to antibiotics. Our platform facilitates the efficient and integrated analysis of multi-plate growth and reporter data. [ABSTRACT FROM AUTHOR]
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Datenbank: Biomedical Index
Beschreibung
Abstract:Researchers traditionally calculate growth rates using the natural logarithm of optical density (OD), with existing script packages facilitating this process. Automatic plate readers, capable of simultaneously measuring OD across 384 cultures, significantly enhance data collection efficiency. Furthermore, these readers also measure luminescence and fluorescence, providing valuable insights into gene expression. However, current analysis software often struggle with data generated by robotic systems measuring multiple plates, limiting the integration of growth and reporter analyses. This method paper addresses three key challenges: (a) the incompatibility of robotic multi-plate systems with existing analysis software, (b) the integration of growth and reporter analyses, and (c) the development of user-friendly interfaces for non-programmers. To address these challenges, we offer optimized script packages and a relevant case study on matrix expression in response to antibiotics. Our platform facilitates the efficient and integrated analysis of multi-plate growth and reporter data. [ABSTRACT FROM AUTHOR]
ISSN:20762607
DOI:10.3390/microorganisms13081889