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(TRANS)ESTERIFICATION OF MANNOSE CATALYZED BY LIPASE B FROM Candida antarctica IN AN IMPROVED REACTION MEDIUM USING CO-SOLVENTS AND MOLECULAR SIEVE.

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Název: (TRANS)ESTERIFICATION OF MANNOSE CATALYZED BY LIPASE B FROM Candida antarctica IN AN IMPROVED REACTION MEDIUM USING CO-SOLVENTS AND MOLECULAR SIEVE.
Autoři: Nott, Katherine, Brognaux, Alison, Richard, Gaëtan, Laurent, Pascal, Favrelle, Audrey, Jérôme, Christine, Blecker, Christophe, Wathelet, Jean-Paul, Paquot, Michel, Deleu, Magali
Zdroj: Preparative Biochemistry & Biotechnology; Jul2012, Vol. 42 Issue 4, p348-363, 16p
Témata: ACYLATION, INITIAL rate method (Chemical kinetics), VINYL ester resins, ESTERS, HYDROXYL group, ENZYMES
Abstrakt: Four co-solvents (dimethylformamide [DMF], formamide, dimethyl sulfoxide [DMSO], and pyridine) were tested with tert-butanol (tBut) to optimize the initial rate (v0) and yield of mannosyl myristate synthesis by esterification catalyzed by immobilized lipase B from Candida antarctica. Ten percent by volume of DMSO resulted in the best improvement of v0 and 48-hr yield (respectively 115% and 13% relative gain compared to pure tBut). Use of molecular sieve (5% w/v) enhances the 48-hr yield (55% in tBut/DMSO [9:1, v/v]). Transesterification in tBut/DMSO (9:1, v/v) with vinyl myristate leads to further improvement of v0 and 48-hr yield: a relative gain of 85% and 65%, respectively, without sieve and 25% and 10%, respectively, with sieve, compared to esterification. No difference in v0 and 48-hr yield is observed when transesterification is carried out with or without sieve. [ABSTRACT FROM AUTHOR]
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Abstrakt:Four co-solvents (dimethylformamide [DMF], formamide, dimethyl sulfoxide [DMSO], and pyridine) were tested with tert-butanol (tBut) to optimize the initial rate (v<subscript>0</subscript>) and yield of mannosyl myristate synthesis by esterification catalyzed by immobilized lipase B from Candida antarctica. Ten percent by volume of DMSO resulted in the best improvement of v<subscript>0</subscript> and 48-hr yield (respectively 115% and 13% relative gain compared to pure tBut). Use of molecular sieve (5% w/v) enhances the 48-hr yield (55% in tBut/DMSO [9:1, v/v]). Transesterification in tBut/DMSO (9:1, v/v) with vinyl myristate leads to further improvement of v<subscript>0</subscript> and 48-hr yield: a relative gain of 85% and 65%, respectively, without sieve and 25% and 10%, respectively, with sieve, compared to esterification. No difference in v<subscript>0</subscript> and 48-hr yield is observed when transesterification is carried out with or without sieve. [ABSTRACT FROM AUTHOR]
ISSN:10826068
DOI:10.1080/10826068.2011.622330