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Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes.

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Názov: Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes.
Autori: York, Andrew G., Ghitani, Alireza, Vaziri, Alipasha, Davidson, Michael W., Shroff, Hari
Zdroj: Nature Methods; Apr2011, Vol. 8 Issue 4, p327-333, 7p, 5 Color Photographs, 1 Graph
Predmety: THREE-dimensional imaging, OPTICAL resolution, LOCALIZATION theory, GRAPHICS processing units, CYTOLOGICAL research, ENDOPLASMIC reticulum
Abstrakt: We demonstrate three-dimensional (3D) super-resolution microscopy in whole fixed cells using photoactivated localization microscopy (PALM). The use of the bright, genetically expressed fluorescent marker photoactivatable monomeric (m)Cherry (PA-mCherry1) in combination with near diffraction-limited confinement of photoactivation using two-photon illumination and 3D localization methods allowed us to investigate a variety of cellular structures at <50 nm lateral and <100 nm axial resolution. Compared to existing methods, we have substantially reduced excitation and bleaching of unlocalized markers, which allows us to use 3D PALM imaging with high localization density in thick structures. Our 3D localization algorithms, which are based on cross-correlation, do not rely on idealized noise models or specific optical configurations. This allows instrument design to be flexible. By generating appropriate fusion constructs and expressing them in Cos7 cells, we could image invaginations of the nuclear membrane, vimentin fibrils, the mitochondrial network and the endoplasmic reticulum at depths of greater than 8 ?m. [ABSTRACT FROM AUTHOR]
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Databáza: Complementary Index
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Abstrakt:We demonstrate three-dimensional (3D) super-resolution microscopy in whole fixed cells using photoactivated localization microscopy (PALM). The use of the bright, genetically expressed fluorescent marker photoactivatable monomeric (m)Cherry (PA-mCherry1) in combination with near diffraction-limited confinement of photoactivation using two-photon illumination and 3D localization methods allowed us to investigate a variety of cellular structures at <50 nm lateral and <100 nm axial resolution. Compared to existing methods, we have substantially reduced excitation and bleaching of unlocalized markers, which allows us to use 3D PALM imaging with high localization density in thick structures. Our 3D localization algorithms, which are based on cross-correlation, do not rely on idealized noise models or specific optical configurations. This allows instrument design to be flexible. By generating appropriate fusion constructs and expressing them in Cos7 cells, we could image invaginations of the nuclear membrane, vimentin fibrils, the mitochondrial network and the endoplasmic reticulum at depths of greater than 8 ?m. [ABSTRACT FROM AUTHOR]
ISSN:15487091
DOI:10.1038/nmeth.1571