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A comparative study between the Chrono-log 700 and the Sysmex CS-2100i analyzers for assessing ristocetin cofactor activity in patients with von Willebrand disease.

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Title: A comparative study between the Chrono-log 700 and the Sysmex CS-2100i analyzers for assessing ristocetin cofactor activity in patients with von Willebrand disease.
Authors: Boulassel, Mohamed-Rachid, Abdellatif, Hussein, Al-Falahi, Karima, Al-Hashmi, Fatma, Bouchareb, Yassine, Al-Abri, Buthaina, Al-Jahdhami, Habib
Source: Journal of Laboratory Medicine; Aug2025, Vol. 49 Issue 4, p149-154, 6p
Subject Terms: BLOOD platelet aggregation, PEARSON correlation (Statistics), PREDICTIVE tests, AUTOANALYZERS, ACADEMIC medical centers, T-test (Statistics), RECEIVER operating characteristic curves, LABORATORIES, BLOOD collection, DESCRIPTIVE statistics, BLOOD coagulation factors, LONGITUDINAL method, INTRACLASS correlation, VON Willebrand disease, AUTOMATION, MEDICAL equipment reliability, COMPARATIVE studies, CONFIDENCE intervals, DATA analysis software, SENSITIVITY & specificity (Statistics), REGRESSION analysis
Geographic Terms: OMAN
Abstract: A variety of methods are currently used to measure von Willebrand factor (VWF) activity, but still the VWF ristocetin cofactor (VWF:RCo) assay using the manual aggregometry technique is the reference method, even having high inter-laboratory variability. The automated coagulation analyzers offer several advantages for routine testing. Herein the performance of the automated Sysmex CS2000/2100i analyzer was compared to the manual aggregometer Chrono-log 700 for assessing VWF:Co activity in patients suspected of having von Willebrand disease (VWD). Plasma samples from 136 patients were prospectively collected, and blindly analyzed on both instruments, simultaneously. Linear regression analysis, Bland-Altman test, intra-class correlation coefficient (ICC), and area under receiver-operator characteristic (ROC) curve were used to evaluate the performance of the automated VWF:RCo assay. There was a strong positive correlation between the two assays (r=0.86, p<0.0001) with an excellent reliability ICC value of 0.81 (95 % CI: 0.74–0.86). A very good degree of agreement between the two assays was also evidenced with an estimated bias of −0.055 (−0.58 to 0.46). The ROC curve for the automated VWF:RCo assay was 0.86 (95 % CI: 0.78–0.92; p<0.0001). Using a cut-off value of 0.44 UI/mL for VWF:RCo activity, the sensitivity and specificity values were 91.2 %, and 88.2 % for the automated assay. The positive and negative positive values for VWD detection were 72.9 %, and 96.7 %, respectively. Collectively, these findings indicate that the automated VWF:RCo assay yields comparable results to the manual aggregometry assay, with very good accuracy and precision to help diagnose patients suspected with VWD. [ABSTRACT FROM AUTHOR]
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Database: Complementary Index
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Abstract:A variety of methods are currently used to measure von Willebrand factor (VWF) activity, but still the VWF ristocetin cofactor (VWF:RCo) assay using the manual aggregometry technique is the reference method, even having high inter-laboratory variability. The automated coagulation analyzers offer several advantages for routine testing. Herein the performance of the automated Sysmex CS2000/2100i analyzer was compared to the manual aggregometer Chrono-log 700 for assessing VWF:Co activity in patients suspected of having von Willebrand disease (VWD). Plasma samples from 136 patients were prospectively collected, and blindly analyzed on both instruments, simultaneously. Linear regression analysis, Bland-Altman test, intra-class correlation coefficient (ICC), and area under receiver-operator characteristic (ROC) curve were used to evaluate the performance of the automated VWF:RCo assay. There was a strong positive correlation between the two assays (r=0.86, p<0.0001) with an excellent reliability ICC value of 0.81 (95 % CI: 0.74–0.86). A very good degree of agreement between the two assays was also evidenced with an estimated bias of −0.055 (−0.58 to 0.46). The ROC curve for the automated VWF:RCo assay was 0.86 (95 % CI: 0.78–0.92; p<0.0001). Using a cut-off value of 0.44 UI/mL for VWF:RCo activity, the sensitivity and specificity values were 91.2 %, and 88.2 % for the automated assay. The positive and negative positive values for VWD detection were 72.9 %, and 96.7 %, respectively. Collectively, these findings indicate that the automated VWF:RCo assay yields comparable results to the manual aggregometry assay, with very good accuracy and precision to help diagnose patients suspected with VWD. [ABSTRACT FROM AUTHOR]
ISSN:25679430
DOI:10.1515/labmed-2024-0160