Preparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein.
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| Title: | Preparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein. |
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| Authors: | He, Jian, Luo, Mingzhan, Zhang, Mengyang, Hu, Xiaomin, Liu, Yangkun, Yao, Lunguang |
| Source: | BMC Veterinary Research; 8/6/2025, Vol. 21 Issue 1, p1-10, 10p |
| Subject Terms: | AFRICAN swine fever, AFRICAN swine fever virus, PROTEIN synthesis, ANTIGENS, MONOCLONAL antibodies, EPITOPES, DIAGNOSIS |
| Abstract: | Background: African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles. Results: In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D, and 8 F were all located in P49-N65, which is highly conserved among genotypes I, II, VII, IX, X, and XX. In contrast, 4B identified an epitope located at K105-R132, which was conserved only in genotypes I, II, and XX ASFV. Conclusions: This study identified two novel conserved epitopes, P49-N65 and K105-R132. This provided valuable insights into the antigenic epitopes of ASFV p15 protein, contributing to a better understanding of its functional properties and demonstrating potential utility for the development of ASFV diagnostic tools. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Abstract: | Background: African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles. Results: In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D, and 8 F were all located in P49-N65, which is highly conserved among genotypes I, II, VII, IX, X, and XX. In contrast, 4B identified an epitope located at K105-R132, which was conserved only in genotypes I, II, and XX ASFV. Conclusions: This study identified two novel conserved epitopes, P49-N65 and K105-R132. This provided valuable insights into the antigenic epitopes of ASFV p15 protein, contributing to a better understanding of its functional properties and demonstrating potential utility for the development of ASFV diagnostic tools. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 17466148 |
| DOI: | 10.1186/s12917-025-04954-y |