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煤层水化学特征对微生物群落结构及代谢功能的影响.

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Názov: 煤层水化学特征对微生物群落结构及代谢功能的影响. (Chinese)
Alternate Title: Impacts of the hydrochemical characteristics of coal seam water on the structures and metabolic functions of microbial communities. (English)
Autori: 王高皓, 段生杰, 王　贝, 牛国斌, 梁永平, 余飞龙, 马　凯, 赵伟仲
Zdroj: Coal Geology & Exploration; May2025, Vol. 53 Issue 5, p104-113, 10p
Predmety: MOTILITY of microorganisms, CARBON content of water, METHANOGENS, BACTERIAL communities, MICROBIAL communities
Abstract (English): [Background] The core of coalbed methane (CBM) bioengineering is to inject bio-fracturing fluids rich in efficient methanogenic bacteria into coal reservoirs to promote the conversion of coals to methane. However, the relationships of the hydrochemical characteristics of coal seam water with the structures and metabolic functions of microbial communities remain unclear. [Methods] This study investigated coal seam water from 12 typical mining areas in Ningxia. Based on microbial classification and sequencing, as well as statistical analysis, this study explored the relationship of pH, anions, cations, and chemical oxygen demand (COD) of coal seam water with the structures and metabolic functions of microbial communities. [Results and Conclusions] The results indicate that the bacterial communities consist primarily of hydrolytic bacteria and bacteria enabling acidogenic fermentation, with dominant genera including Proteiniborus, Clostridium_sensu_stricto_1, and Thauera. The metabolism of methanogenic archaea occurs primarily through mixotrophy, with Methanosarcina identified as the dominant genus of these archaea. The coal seam water exhibits pH values ranging from 7.4 to 8.5. A decrease in pH value corresponds to increased diversity of the bacterial communities. However, the pH value exerts a small impact on the archaeal communities and an insignificant impact on the microbial metabolic functions. The diversity and abundance of the bacterial communities are positively correlated with the mass concentrations of anions Cl− and SO42- when their mass concentrations are less than 905 mg/L and 1 974 mg/L, respectively. In contrast, the mass concentrations of cations Ca2+ and Mg2+ are significantly negatively correlated with microbial cell motility, intracellular transport, secretion, and vesicular transport, as well as the metabolism of inorganic ions, when their mass concentrations range from 5.6 mg/L to 411.0 mg/L and from 30.3 mg/L to 697.0 mg/L, respectively. Additionally, the COD in coal seam water exhibits significant positive correlations with the energy generation and transformation, along with the carbon cycle, involving microbial communities. Higher COD is associated with richer organic matter in water, thereby enhancing the involvement of microbial communities in the carbon cycle. The results of this study reveal the mechanisms behind the impacts of the hydrochemical characteristics of coal seam water on microbial communities while also laying a scientific basis for optimizing the formulation of bio-fracturing fluids in CBM bioengineering. Moreover, these results help understand the potential impacts of environmental changes on underground microbial communities during CBM production. [ABSTRACT FROM AUTHOR]
Abstract (Chinese): [背景] 煤层气生物工程的核心在于向煤储层注入富含高效产甲烷菌群的生物压裂液, 以促 进煤向甲烷的转化。然而, 现阶段对煤层水的化学特征、微生物群落结构及其代谢功能之间的关系 尚不明确。 [方法] 以宁夏 12 个典型矿区的煤层水为研究对象, 基于微生物分类测序和统计学分析 的方法探讨煤层水中的 pH、阴离子、阳离子、化学需氧量(COD) 与微生物群落结构及代谢功能之间 的关联。 [结果和结论] (1) 细菌群落主要由水解菌和产酸发酵菌组成, 优势属为 Proteiniborus、 Clostridium_sensu_stricto_1 和 Thauera; 产甲烷古菌主要通过混合营养型代谢, 优势属为 Methanosarcina。(2) 煤层水的 pH 为 7.4~8.5, 随着 pH 降低, 细菌群落的多样性增加, 而 pH 值对古菌群 落的影响较小, 对微生物代谢功能无显著影响。(3) 当 Cl−质量浓度低于 905 mg/L、 SO2-4质量浓度低 于 1 974 mg/L 时, 细菌群落的多样性和丰度与这些阴离子浓度呈正相关; 而当 Ca2+质量浓度在 5.6~411.0 mg/L、Mg2+浓度在 30.3~697.0 mg/L 时, 这些阳离子浓度与微生物的细胞运动、细胞内运 输、分泌与囊泡运输以及无机离子代谢表现出显著负相关性。(4) 此外, 煤层水中的 COD 与微生物 群落参与的能量生成、转化及碳循环呈显著正相关关系, COD 越高, 水中的有机质越丰富, 进而增 强微生物群落对碳循环的参与。研究成果不仅揭示了煤层水化学特征对微生物群落的影响机制, 还 为煤层气生物工程中生物压裂液配方的优化提供了科学依据, 帮助理解煤层气开发过程中环境变化 对地下微生物群落的潜在影响。 [ABSTRACT FROM AUTHOR]
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Abstrakt:[Background] The core of coalbed methane (CBM) bioengineering is to inject bio-fracturing fluids rich in efficient methanogenic bacteria into coal reservoirs to promote the conversion of coals to methane. However, the relationships of the hydrochemical characteristics of coal seam water with the structures and metabolic functions of microbial communities remain unclear. [Methods] This study investigated coal seam water from 12 typical mining areas in Ningxia. Based on microbial classification and sequencing, as well as statistical analysis, this study explored the relationship of pH, anions, cations, and chemical oxygen demand (COD) of coal seam water with the structures and metabolic functions of microbial communities. [Results and Conclusions] The results indicate that the bacterial communities consist primarily of hydrolytic bacteria and bacteria enabling acidogenic fermentation, with dominant genera including Proteiniborus, Clostridium_sensu_stricto_1, and Thauera. The metabolism of methanogenic archaea occurs primarily through mixotrophy, with Methanosarcina identified as the dominant genus of these archaea. The coal seam water exhibits pH values ranging from 7.4 to 8.5. A decrease in pH value corresponds to increased diversity of the bacterial communities. However, the pH value exerts a small impact on the archaeal communities and an insignificant impact on the microbial metabolic functions. The diversity and abundance of the bacterial communities are positively correlated with the mass concentrations of anions Cl<sup>−</sup> and SO<subscript>4</subscript><sup>2-</sup> when their mass concentrations are less than 905 mg/L and 1 974 mg/L, respectively. In contrast, the mass concentrations of cations Ca<sup>2+</sup> and Mg<sup>2+</sup> are significantly negatively correlated with microbial cell motility, intracellular transport, secretion, and vesicular transport, as well as the metabolism of inorganic ions, when their mass concentrations range from 5.6 mg/L to 411.0 mg/L and from 30.3 mg/L to 697.0 mg/L, respectively. Additionally, the COD in coal seam water exhibits significant positive correlations with the energy generation and transformation, along with the carbon cycle, involving microbial communities. Higher COD is associated with richer organic matter in water, thereby enhancing the involvement of microbial communities in the carbon cycle. The results of this study reveal the mechanisms behind the impacts of the hydrochemical characteristics of coal seam water on microbial communities while also laying a scientific basis for optimizing the formulation of bio-fracturing fluids in CBM bioengineering. Moreover, these results help understand the potential impacts of environmental changes on underground microbial communities during CBM production. [ABSTRACT FROM AUTHOR]
ISSN:10011986
DOI:10.12363/issn.1001-1986.24.11.0691