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Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China.

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Bibliographic Details
Title: Stool carriage of CTX-M/CMY-producing Salmonella enterica in a Chinese tertiary hospital in Shenzhen, China.
Authors: Wang, Jing, Dong, Zi-Han, Zhou, Xian-Yuan, Ma, Qin-Chun, Wang, Zhen-Yu, Lin, Dachuan, Huang, Ying-Feng, Zhang, Chi, Jiao, Xinan, Li, Deng, Li, Qiuchun
Source: Frontiers in Cellular & Infection Microbiology; 2025, p1-13, 13p
Subject Terms: SALMONELLA diseases, MOBILE genetic elements, WHOLE genome sequencing, SALMONELLA enterica, ESCHERICHIA coli
Abstract: Salmonellosis, caused by non-typhoidal Salmonella , is a common foodborne gastrointestinal infection. Third-generation cephalosporins are recommended as the first-line treatment for Salmonella infections. Our study aimed to investigate the molecular epidemiology, antimicrobial resistance, and the transmission of extended-spectrum β-lactamases (ESBL) genes in 96 clinical Salmonella isolates collected between 2020 and 2022 at a tertiary hospital in Shenzhen, China. We performed antimicrobial susceptibility testing and whole-genome sequencing to identify serotypes, multilocus sequence typing, antimicrobial resistance genes in these isolates, and the genetic structures of the bla CTX-M/ bla CMY genes. Seventeen Salmonella serotypes were identified, with S. 4,[5],12:i:- (37.5%) being the most common, followed by S. Enteritidis (15.63%), S. Typhimurium (14.58%), S. London (7.29%), and S. Rissen (5.21%). MLST analysis revealed 19 distinct sequence types (STs), with ST34 being the most prevalent (36.46%), followed by ST11 (15.63%) and ST19 (13.54%). Antimicrobial resistance testing showed those isolates had high levels of resistance to ampicillin (72.92%) and tetracycline (71.88%), with 70.83% of isolates as multidrug-resistant (MDR). Three bla CTX-M genes (bla CTX-M-14, bla CTX-M-55, and bla CTX-M-65) and bla CMY-2 were identified among 18 cefotaxime-resistant strains, of which one and 12 isolates successfully transferred bla CMY or bla CTX-M to E. coli C600 via conjugation, respectively. The bla CTX-M/ bla CMY-2-carrying contigs in nine Salmonella isolates ranged from 2,156 to 164,862 bp, were located either on the chromosome (n=1) or plasmids (IncI1, IncK1, IncA/C) (n=9), and the bla CTX-M/ bla CMY-2 genes were associated with IS Ecp1. Our study demonstrates the diversity of MDR Salmonella serotypes in clinical isolates, and highlights the role of plasmids and mobile genetic elements in the horizontal transfer of bla CTX-M/ bla CMY, emphasizing the need for continuous surveillance of Salmonella in clinical samples. [ABSTRACT FROM AUTHOR]
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Database: Complementary Index
Description
Abstract:Salmonellosis, caused by non-typhoidal Salmonella , is a common foodborne gastrointestinal infection. Third-generation cephalosporins are recommended as the first-line treatment for Salmonella infections. Our study aimed to investigate the molecular epidemiology, antimicrobial resistance, and the transmission of extended-spectrum β-lactamases (ESBL) genes in 96 clinical Salmonella isolates collected between 2020 and 2022 at a tertiary hospital in Shenzhen, China. We performed antimicrobial susceptibility testing and whole-genome sequencing to identify serotypes, multilocus sequence typing, antimicrobial resistance genes in these isolates, and the genetic structures of the bla <subscript>CTX-M</subscript>/ bla <subscript>CMY</subscript> genes. Seventeen Salmonella serotypes were identified, with S. 4,[5],12:i:- (37.5%) being the most common, followed by S. Enteritidis (15.63%), S. Typhimurium (14.58%), S. London (7.29%), and S. Rissen (5.21%). MLST analysis revealed 19 distinct sequence types (STs), with ST34 being the most prevalent (36.46%), followed by ST11 (15.63%) and ST19 (13.54%). Antimicrobial resistance testing showed those isolates had high levels of resistance to ampicillin (72.92%) and tetracycline (71.88%), with 70.83% of isolates as multidrug-resistant (MDR). Three bla <subscript>CTX-M</subscript> genes (bla <subscript>CTX-M-14,</subscript> bla <subscript>CTX-M-55</subscript>, and bla <subscript>CTX-M-65</subscript>) and bla <subscript>CMY-2</subscript> were identified among 18 cefotaxime-resistant strains, of which one and 12 isolates successfully transferred bla <subscript>CMY</subscript> or bla <subscript>CTX-M</subscript> to E. coli C600 via conjugation, respectively. The bla <subscript>CTX-M</subscript>/ bla <subscript>CMY-2</subscript>-carrying contigs in nine Salmonella isolates ranged from 2,156 to 164,862 bp, were located either on the chromosome (n=1) or plasmids (IncI1, IncK1, IncA/C) (n=9), and the bla <subscript>CTX-M</subscript>/ bla <subscript>CMY-2</subscript> genes were associated with IS Ecp1. Our study demonstrates the diversity of MDR Salmonella serotypes in clinical isolates, and highlights the role of plasmids and mobile genetic elements in the horizontal transfer of bla <subscript>CTX-M</subscript>/ bla <subscript>CMY</subscript>, emphasizing the need for continuous surveillance of Salmonella in clinical samples. [ABSTRACT FROM AUTHOR]
ISSN:22352988
DOI:10.3389/fcimb.2025.1544757