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Neutrophil extracellular traps in rheumatoid arthritis and periodontitis: Contribution of PADI4 gene polymorphisms.

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Název: Neutrophil extracellular traps in rheumatoid arthritis and periodontitis: Contribution of PADI4 gene polymorphisms.
Autoři: Oliveira, Sicília Rezende, de Arruda, José Alcides Almeida, Schneider, Ayda Henriques, Bemquerer, Larissa Marques, de Souza, Rayssa Maria Soalheiro, Barbim, Paula, de Mattos‐Pereira, Gustavo Henrique, Calderaro, Débora Cerqueira, Machado, Caio Cavalcante, Alves, Sandra Fukada, Moreira, Paula Rocha, de Oliveira, Renê Donizeti Ribeiro, Louzada‐Júnior, Paulo, Abreu, Lucas Guimarães, Cunha, Fernando Queiroz, Silva, Tarcília Aparecida
Zdroj: Journal of Clinical Periodontology; Apr2024, Vol. 51 Issue 4, p452-463, 12p
Témata: HYDROLASES, IN vitro studies, ARGININE, NEUTROPHILS, RHEUMATOID arthritis, GINGIVA, FLUORESCENT antibody technique, GENETIC polymorphisms, HISTONES, EXTRACELLULAR space, GRAM-negative anaerobic bacteria, HAPLOTYPES, CONNECTIVE tissues, SINGLE nucleotide polymorphisms, PERIODONTITIS
Abstrakt: Aim: We sought to investigate the release of neutrophil extracellular traps (NETs) in neutrophils from individuals with rheumatoid arthritis (RA) and controls and compare the presence of NETs in gingival tissues according to periodontal status. Also, the association between single nucleotide polymorphisms (SNPs) of the peptidyl arginine deaminase type 4 (PADI4) gene and the GTG haplotype with RA, periodontitis and NETs was evaluated in vitro. Materials and Methods: Peripheral neutrophils were isolated by density gradient, and NET concentration was determined by the PicoGreen method. Immunofluorescence was studied to identify NETs by co‐localization of myeloperoxidase (MPO)‐citrullinated histone H3 (H3Cit). Genotyping for SNPs (PADI4_89; PADI4_90; PADI4_92; and PADI4_104) was performed in 87 individuals with RA and 111 controls. Results: The release of NETs in vitro was significantly higher in individuals with RA and periodontitis and when stimulated with Porphyromonas gingivalis. Gingival tissues from subjects with RA and periodontitis revealed increased numbers of MPO‐H3Cit‐positive cells. Individuals with the GTG haplotype showed a higher release of NETs in vitro and worse periodontal parameters. Conclusions: The release of NETs by circulating neutrophils is associated with RA and periodontitis and is influenced by the presence of the GTG haplotype. [ABSTRACT FROM AUTHOR]
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Databáze: Complementary Index
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Abstrakt:Aim: We sought to investigate the release of neutrophil extracellular traps (NETs) in neutrophils from individuals with rheumatoid arthritis (RA) and controls and compare the presence of NETs in gingival tissues according to periodontal status. Also, the association between single nucleotide polymorphisms (SNPs) of the peptidyl arginine deaminase type 4 (PADI4) gene and the GTG haplotype with RA, periodontitis and NETs was evaluated in vitro. Materials and Methods: Peripheral neutrophils were isolated by density gradient, and NET concentration was determined by the PicoGreen method. Immunofluorescence was studied to identify NETs by co‐localization of myeloperoxidase (MPO)‐citrullinated histone H3 (H3Cit). Genotyping for SNPs (PADI4_89; PADI4_90; PADI4_92; and PADI4_104) was performed in 87 individuals with RA and 111 controls. Results: The release of NETs in vitro was significantly higher in individuals with RA and periodontitis and when stimulated with Porphyromonas gingivalis. Gingival tissues from subjects with RA and periodontitis revealed increased numbers of MPO‐H3Cit‐positive cells. Individuals with the GTG haplotype showed a higher release of NETs in vitro and worse periodontal parameters. Conclusions: The release of NETs by circulating neutrophils is associated with RA and periodontitis and is influenced by the presence of the GTG haplotype. [ABSTRACT FROM AUTHOR]
ISSN:03036979
DOI:10.1111/jcpe.13921