Zobrazit v EDS

Analysis method of cellular stress caused by intermediate dose‐rate irradiation using a cell lysate array technique.

Uloženo v:
Podrobná bibliografie
Název: Analysis method of cellular stress caused by intermediate dose‐rate irradiation using a cell lysate array technique.
Autoři: Morishima, Nobuhiro, Ogata, Hiromitsu, Magae, Junji, Ito, Yoshihiro, Kobayashi, Junya
Zdroj: Genes to Cells; Apr2023, Vol. 28 Issue 4, p288-306, 19p
Témata: TISSUE arrays, CELL analysis, CELL physiology, BIOMOLECULES, PROTEIN microarrays, DNA damage
Abstrakt: Ionizing radiation damages DNA and may lead to the development of cancer. Irradiation also generates reactive oxygen species (ROS) which cause damage to various biological molecules. Relatively low dose‐rate irradiation causes less damage. However, the damage and its effects on cell fate are difficult to evaluate. To develop a method to analyze the damage and accompanying changes in physiology in cells irradiated by γ‐rays at a relatively low dose‐rate, we used the protein array technique to quantify marker proteins involved in the stress response and the regulation of cell growth and death. This method enabled efficient analyses of many replicates of experimental data on cell lysate samples. We detected relatively small changes in the levels of these proteins in the irradiated cells. Changes in protein levels suggested ROS production and DNA damage as well as cell cycle retardation and the progression of cellular senescence. Thus, our approach shows promise for analyzing the biological effects of relatively low dose‐rate irradiation. [ABSTRACT FROM AUTHOR]
Copyright of Genes to Cells is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Databáze: Complementary Index
Popis
Abstrakt:Ionizing radiation damages DNA and may lead to the development of cancer. Irradiation also generates reactive oxygen species (ROS) which cause damage to various biological molecules. Relatively low dose‐rate irradiation causes less damage. However, the damage and its effects on cell fate are difficult to evaluate. To develop a method to analyze the damage and accompanying changes in physiology in cells irradiated by γ‐rays at a relatively low dose‐rate, we used the protein array technique to quantify marker proteins involved in the stress response and the regulation of cell growth and death. This method enabled efficient analyses of many replicates of experimental data on cell lysate samples. We detected relatively small changes in the levels of these proteins in the irradiated cells. Changes in protein levels suggested ROS production and DNA damage as well as cell cycle retardation and the progression of cellular senescence. Thus, our approach shows promise for analyzing the biological effects of relatively low dose‐rate irradiation. [ABSTRACT FROM AUTHOR]
ISSN:13569597
DOI:10.1111/gtc.13011