Zobraziť v EDS

Zanthoxylum bungeanum Essential Oil: Extraction and Component Analysis for α-Glucosidase Inhibitory Activity and the Underlying Mechanism Based on Molecular Docking.

Uložené v:
Podrobná bibliografia
Názov: Zanthoxylum bungeanum Essential Oil: Extraction and Component Analysis for α-Glucosidase Inhibitory Activity and the Underlying Mechanism Based on Molecular Docking.
Autori: Liang, Shaoqi, Hu, Wei, Cheng, Wensi, Zhang, Sheng, Zou, Ruisi
Zdroj: Applied Sciences (2076-3417); Feb2023, Vol. 13 Issue 4, p2627, 11p
Predmety: GAS chromatography/Mass spectrometry (GC-MS), ESSENTIAL oils, ALPHA-glucosidases, MOLECULAR docking, ZANTHOXYLUM, VEGETABLE oils, CHOLINE chloride, BINDING energy
Abstrakt: Zanthoxylum bungeanum essential oil was extracted using a low-eutectic solvent (choline chloride and 1,3-butanediol) and steam distillation. The results showed that the yield (9.36 and 10.00%) did not differ significantly between the two methods. The α-glucosidase inhibitory activity of essential oils from 20 species was screened by the PNPG (4-p-nitrophenyl-a-d-glucopyranosylase) colorimetric method, and the results showed that the inhibition rate of Zanthoxylum bungeanum essential oil reached 57.1%, far more than those of the other essential oils tested. The main components and contents of Zanthoxylum bungeanum essential oil were terpineol-4-ol (13.13%), (-)-β-pinene (11.17%), γ-terpinene (9.45%), terpinyl acetate (9.36%), and α-terpineol (5.40%), which were identified by GC-MS (gas chromatography–mass spectrometry). According to the literature, linalool and limonene are also high-content components. These seven components were docked with the α-glucosidase molecule. It was found that the binding energies between terpineol-4-ol, (-)-β-pinene, γ-terpinene, terpinyl acetate, α-terpineol, linalool, limonene, and α-glucosidase were −28.88, −25.54, −26.37, −28.46, −27.63, −25.95, and −25.53 (KJ/mol), respectively, similar to those of anthocyanins. From the research results, the following conclusions can be drawn: the extraction rate of Zanthoxylum bungeanum essential oil can be increased with steam distillation. Of the many plant essential oils, Zanthoxylum bungeanum essential oil shows excellent anti-α-glucosidase activity, suggesting that it has potential for application in hypoglycemic drugs. The strong binding ability of the monoterpene-based chemical components with the α-glucosidase molecule revealed the chemical mechanism of the inhibitory effect of Zanthoxylum bungeanum essential oil on α-glucosidase. The above conclusions provide a theoretical basis for the utilization of Zanthoxylum bungeanum essential oil in hypoglycemic drugs and further expand the applications of small-molecule chemical components. [ABSTRACT FROM AUTHOR]
Copyright of Applied Sciences (2076-3417) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Databáza: Complementary Index
Popis
Abstrakt:Zanthoxylum bungeanum essential oil was extracted using a low-eutectic solvent (choline chloride and 1,3-butanediol) and steam distillation. The results showed that the yield (9.36 and 10.00%) did not differ significantly between the two methods. The α-glucosidase inhibitory activity of essential oils from 20 species was screened by the PNPG (4-p-nitrophenyl-a-d-glucopyranosylase) colorimetric method, and the results showed that the inhibition rate of Zanthoxylum bungeanum essential oil reached 57.1%, far more than those of the other essential oils tested. The main components and contents of Zanthoxylum bungeanum essential oil were terpineol-4-ol (13.13%), (-)-β-pinene (11.17%), γ-terpinene (9.45%), terpinyl acetate (9.36%), and α-terpineol (5.40%), which were identified by GC-MS (gas chromatography–mass spectrometry). According to the literature, linalool and limonene are also high-content components. These seven components were docked with the α-glucosidase molecule. It was found that the binding energies between terpineol-4-ol, (-)-β-pinene, γ-terpinene, terpinyl acetate, α-terpineol, linalool, limonene, and α-glucosidase were −28.88, −25.54, −26.37, −28.46, −27.63, −25.95, and −25.53 (KJ/mol), respectively, similar to those of anthocyanins. From the research results, the following conclusions can be drawn: the extraction rate of Zanthoxylum bungeanum essential oil can be increased with steam distillation. Of the many plant essential oils, Zanthoxylum bungeanum essential oil shows excellent anti-α-glucosidase activity, suggesting that it has potential for application in hypoglycemic drugs. The strong binding ability of the monoterpene-based chemical components with the α-glucosidase molecule revealed the chemical mechanism of the inhibitory effect of Zanthoxylum bungeanum essential oil on α-glucosidase. The above conclusions provide a theoretical basis for the utilization of Zanthoxylum bungeanum essential oil in hypoglycemic drugs and further expand the applications of small-molecule chemical components. [ABSTRACT FROM AUTHOR]
ISSN:20763417
DOI:10.3390/app13042627