View in EDS

HLA graph, a free and ready‐to‐use bioinformatics tool to explore anti‐HLA eplets reactivity pattern.

Saved in:
Bibliographic Details
Title: HLA graph, a free and ready‐to‐use bioinformatics tool to explore anti‐HLA eplets reactivity pattern.
Authors: Usureau, Cédric, Jacob, Valentine, Dubois, Valérie, Masson, Dominique, Jollet, Isabelle, Desoutter, Judith, Taupin, Jean‐Luc, Guillaume, Nicolas
Source: HLA: Immune Response Genetics; Sep2022, Vol. 100 Issue 3, p244-253, 10p
Subject Terms: HLA histocompatibility antigens, WEB accessibility, HYPERLINKS, BIOINFORMATICS software, ANTIBODY titer, WEB browsers, SHORT tandem repeat analysis
Abstract: Introduction: HLA antigens are highly polymorphic, and their immunogenicity is dependent on the configurations of polymorphic amino acids. Monitoring anti‐HLA immunization is essential in organ transplantation, as antibodies directed against HLA molecules are a major cause of rejection. Anti‐HLA antibodies are not specific for HLA antigens, but recognize B‐cell epitopes present on HLA molecules. Methods: To better understand antibody reactivity patterns, we calculated the Spearman correlation of the mean fluorescence intensity (MFI) of anti‐HLA antibodies identified by a single‐antigen assay performed using a Luminex® immunobeads assay on a large number of samples. Then, we built a computer tool analyzing antibody reactivity patterns with an accessibility by a web browser linked to the International Epitope Registry. We also extended our model to Onelambda® and Lifecodes® single‐antigen class I and class II assays. Results and Discussion: The resulting MFI correlations reflect HLA antibody cross‐reactivity and eplets similarity. We built HLA Graph, a computer tool that analyzes the eplets involved in antibody reactivity profiles. HLA Graph is usable with Onelambda® and Lifecodes® single‐antigen class I and class II assays. The interpretation of reactivity against alleles not tested by the antibody assays and against the alpha and beta chains of HLA‐DQ and HLA‐DP loci were also developed. Conclusion: HLA Graph is a free and ready‐to‐use bioinformatics tool that can be used by all laboratories performing anti‐HLA antibody identification by immunobead assay. [ABSTRACT FROM AUTHOR]
Copyright of HLA: Immune Response Genetics is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Complementary Index
Description
Abstract:Introduction: HLA antigens are highly polymorphic, and their immunogenicity is dependent on the configurations of polymorphic amino acids. Monitoring anti‐HLA immunization is essential in organ transplantation, as antibodies directed against HLA molecules are a major cause of rejection. Anti‐HLA antibodies are not specific for HLA antigens, but recognize B‐cell epitopes present on HLA molecules. Methods: To better understand antibody reactivity patterns, we calculated the Spearman correlation of the mean fluorescence intensity (MFI) of anti‐HLA antibodies identified by a single‐antigen assay performed using a Luminex® immunobeads assay on a large number of samples. Then, we built a computer tool analyzing antibody reactivity patterns with an accessibility by a web browser linked to the International Epitope Registry. We also extended our model to Onelambda® and Lifecodes® single‐antigen class I and class II assays. Results and Discussion: The resulting MFI correlations reflect HLA antibody cross‐reactivity and eplets similarity. We built HLA Graph, a computer tool that analyzes the eplets involved in antibody reactivity profiles. HLA Graph is usable with Onelambda® and Lifecodes® single‐antigen class I and class II assays. The interpretation of reactivity against alleles not tested by the antibody assays and against the alpha and beta chains of HLA‐DQ and HLA‐DP loci were also developed. Conclusion: HLA Graph is a free and ready‐to‐use bioinformatics tool that can be used by all laboratories performing anti‐HLA antibody identification by immunobead assay. [ABSTRACT FROM AUTHOR]
ISSN:20592302
DOI:10.1111/tan.14701