Dual-Receptor Recognition, Lysis Inhibition, Endolysin Release, and Reaction-Diffusion as Alternative Explanations. Comment on Rojero et al. Bypassing Evolution of Bacterial Resistance to Phages: The Example of Hyper-Aggressive Phage 0524phi7-1. Int. J. Mol. Sci. 2025, 26 , 2914.
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| Názov: | Dual-Receptor Recognition, Lysis Inhibition, Endolysin Release, and Reaction-Diffusion as Alternative Explanations. Comment on Rojero et al. Bypassing Evolution of Bacterial Resistance to Phages: The Example of Hyper-Aggressive Phage 0524phi7-1. Int. J. Mol. Sci. 2025, 26 , 2914. |
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| Autori: | Abedon ST; Department of Microbiology, The Ohio State University, Mansfield, OH 44906, USA. |
| Zdroj: | International journal of molecular sciences [Int J Mol Sci] 2025 Nov 25; Vol. 26 (23). Date of Electronic Publication: 2025 Nov 25. |
| Spôsob vydávania: | Comment; Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, [2000- |
| Výrazy zo slovníka MeSH: | Bacteriophages*/physiology , Endopeptidases*/metabolism , Bacteriolysis* , Bacteria*/virology |
| Abstrakt: | Presented here are additional explanations for five key points offered by Rojero et al. in their 2025 publication in this journal, regarding characteristics of hyper-aggressive phage 0524phi7-1. These are (i) that the "bypassing of the evolution of host resistance" has been seen in other phages, especially dual-receptor generalist phages; (ii) that the "clearing of semi-turbid plaques" could be due to a phenomenon known as lysis inhibition collapse, (iii) that the "formation of satellite plaques" is reminiscent of the morphology of plaques generated by phage T4 star mutants, (iv) that "multi-day plaque enlargement" has been seen in other phages such as phage T7 but may also be explained by other phenomena including endolysin release, (v) that suggestions of phage "swimming" could be explained by virion diffusion within empty volumes found within maturing bacterial lawns. In particular, phage plaques that display lysis inhibition can influence the surrounding bacterial lawn well beyond their visible region. This presumably occurs via a reaction-diffusion mechanism whose leading edge of virion diffusion fails to display lysis inhibition, but which leaves in its wake lysis-inhibited bacterial infections that may not lyse in a timely manner. Phage-infected bacteria thus may be found well beyond a plaque's visible boundaries, along with diffusing endolysin. |
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| Grant Information: | R01 AI169865 United States AI NIAID NIH HHS |
| Contributed Indexing: | Keywords: dual-receptor generalist; endolysin; lysin; lysis inhibition; lysis-inhibition collapse; reaction-diffusion; star mutants |
| Substance Nomenclature: | EC 3.4.99.- (endolysin) EC 3.4.- (Endopeptidases) |
| Entry Date(s): | Date Created: 20251211 Date Completed: 20251211 Latest Revision: 20251216 |
| Update Code: | 20251216 |
| PubMed Central ID: | PMC12692476 |
| DOI: | 10.3390/ijms262311368 |
| PMID: | 41373527 |
| Databáza: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Presented here are additional explanations for five key points offered by Rojero et al. in their 2025 publication in this journal, regarding characteristics of hyper-aggressive phage 0524phi7-1. These are (i) that the "bypassing of the evolution of host resistance" has been seen in other phages, especially dual-receptor generalist phages; (ii) that the "clearing of semi-turbid plaques" could be due to a phenomenon known as lysis inhibition collapse, (iii) that the "formation of satellite plaques" is reminiscent of the morphology of plaques generated by phage T4 star mutants, (iv) that "multi-day plaque enlargement" has been seen in other phages such as phage T7 but may also be explained by other phenomena including endolysin release, (v) that suggestions of phage "swimming" could be explained by virion diffusion within empty volumes found within maturing bacterial lawns. In particular, phage plaques that display lysis inhibition can influence the surrounding bacterial lawn well beyond their visible region. This presumably occurs via a reaction-diffusion mechanism whose leading edge of virion diffusion fails to display lysis inhibition, but which leaves in its wake lysis-inhibited bacterial infections that may not lyse in a timely manner. Phage-infected bacteria thus may be found well beyond a plaque's visible boundaries, along with diffusing endolysin. |
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| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms262311368 |