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Myoinositol enhances heat tolerance in Chenopodium quinoa through integrated physiological, biochemical, and molecular responses.

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Title: Myoinositol enhances heat tolerance in Chenopodium quinoa through integrated physiological, biochemical, and molecular responses.
Authors: Alshammari AA; Department of Biology, College of Science, University of Hail, Hail 2440, Saudi Arabia. Email: a.ashammar@uoh.edu.sa, W.alshamary@uoh.edu.sa., Abu-Elsaoud AM; Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia. Email: amsmohamed@imamu.edu.sa, harudayni@imamu.edu.sa, mmyalzahrani@imamu.edu.sa., AlShammari W; Department of Biology, College of Science, University of Hail, Hail 2440, Saudi Arabia. Email: a.ashammar@uoh.edu.sa, W.alshamary@uoh.edu.sa., Abdulmajeed AM; Biology Department, Faculty of Science, University of Tabuk, Umluj, Tabuk 46429, Saudi Arabia. Email: awabdulmajeed@ut.edu.sa., Aysh ALrashidi A; Department of Biology, Faculty of Science, University of Hail, Hail 81411, Saudi Arabia. Email: ais.alrashydy@uoh.edu.sa., Alghanem SMS; Department of Biology, College of Science, Qassim University, Burydah 52571, Saudi Arabia. Email: su.alghanem@qu.edu.sa., Rudayni H; Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia. Email: amsmohamed@imamu.edu.sa, harudayni@imamu.edu.sa, mmyalzahrani@imamu.edu.sa., Al-Zharani M; Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia. Email: amsmohamed@imamu.edu.sa, harudayni@imamu.edu.sa, mmyalzahrani@imamu.edu.sa., Alnusaire TS; Department of Biology, College of Science, Jouf University, Sakaka 2014, Saudi Arabia. Email: tasalnosairi@ju.edu.sa., Soliman MH; Department of Biology, College of Science in Yanbu, Taibah University, Madinah, Saudi Arabia. Email: Email: mhhussein@taibahu.edu.sa.; Botany and Microbiology Department, Faculty of Science, Cairo University, Giza 12613, Egypt. Email: hmona@sci.cu.edu.eg.
Source: Functional plant biology : FPB [Funct Plant Biol] 2025 Dec 23; Vol. 52 (12).
Publication Type: Journal Article
Language: English
Journal Info: Publisher: CSIRO Pub Country of Publication: Australia NLM ID: 101154361 Publication Model: Print Cited Medium: Internet ISSN: 1445-4416 (Electronic) Linking ISSN: 14454416 NLM ISO Abbreviation: Funct Plant Biol Subsets: MEDLINE
Imprint Name(s): Original Publication: Collingwood, VIC, Australia : CSIRO Pub., c2002-
MeSH Terms: Chenopodium quinoa*/physiology , Chenopodium quinoa*/drug effects , Chenopodium quinoa*/metabolism , Chenopodium quinoa*/genetics , Inositol*/pharmacology , Inositol*/metabolism , Thermotolerance*/drug effects, Oxidative Stress/drug effects ; Gene Expression Regulation, Plant/drug effects ; Heat-Shock Response/drug effects ; Photosynthesis/drug effects
Abstract: As global temperatures continue to rise, heat stress poses a serious threat to crop productivity and food security. Developing effective strategies to enhance thermotolerance is therefore critical. This study investigates the physiological, biochemical, and molecular mechanisms underlying the protective role of myo-inositol (MyIs) in enhancing heat tolerance in Chenopodium quinoa exposed to varying thermal stress conditions (30°C, 35°C, and 40°C), with or without MyIs application. Heat stress markedly reduced biomass accumulation, relative water content (RWC), photosynthetic efficiency, and membrane stability, while increasing oxidative damage, as evidenced by elevated malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide radicals (O2•-), and electrolyte leakage (EL). MyIs significantly mitigated these adverse effects by enhancing shoot and root growth, maintaining higher RWC and photosynthetic performance, and reducing oxidative stress markers. Biochemical profiling revealed restoration of total soluble sugars, protein, and free amino acids, alongside elevated glycine betaine (GB) and abscisic acid (ABA), contributing to improved osmotic adjustment and stress signaling. Gene expression analysis showed upregulation of key heat-responsive genes (sHSP18.1, HSP20, HSP70, HSP83, and NSY), indicating a transcriptional basis for MyIs-induced heat tolerance. Collectively, these findings demonstrate that MyIs functions as a potent bio-regulator, conferring thermoprotection through integrated physiological and molecular responses, and offers promising potential for improving heat resilience in crops.
(© 2025 The Author(s) (or their employer(s)). Published by CSIRO Publishing.)
Contributed Indexing: Keywords: ABA signaling; Myoinositol; antioxidant defense; heat shock proteins (HSPs); heat stress; osmotic adjustment; oxidative stress; photosynthetic efficiency; physiological and biochemical responses; stress-responsive genes; thermotolerance mechanisms
Substance Nomenclature: 4L6452S749 (Inositol)
Entry Date(s): Date Created: 20251126 Date Completed: 20251127 Latest Revision: 20251127
Update Code: 20251128
DOI: 10.1071/FP25281
PMID: 41292009
Database: MEDLINE
Description
Abstract:As global temperatures continue to rise, heat stress poses a serious threat to crop productivity and food security. Developing effective strategies to enhance thermotolerance is therefore critical. This study investigates the physiological, biochemical, and molecular mechanisms underlying the protective role of myo-inositol (MyIs) in enhancing heat tolerance in Chenopodium quinoa exposed to varying thermal stress conditions (30°C, 35°C, and 40°C), with or without MyIs application. Heat stress markedly reduced biomass accumulation, relative water content (RWC), photosynthetic efficiency, and membrane stability, while increasing oxidative damage, as evidenced by elevated malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide radicals (O2•-), and electrolyte leakage (EL). MyIs significantly mitigated these adverse effects by enhancing shoot and root growth, maintaining higher RWC and photosynthetic performance, and reducing oxidative stress markers. Biochemical profiling revealed restoration of total soluble sugars, protein, and free amino acids, alongside elevated glycine betaine (GB) and abscisic acid (ABA), contributing to improved osmotic adjustment and stress signaling. Gene expression analysis showed upregulation of key heat-responsive genes (sHSP18.1, HSP20, HSP70, HSP83, and NSY), indicating a transcriptional basis for MyIs-induced heat tolerance. Collectively, these findings demonstrate that MyIs functions as a potent bio-regulator, conferring thermoprotection through integrated physiological and molecular responses, and offers promising potential for improving heat resilience in crops.<br /> (© 2025 The Author(s) (or their employer(s)). Published by CSIRO Publishing.)
ISSN:1445-4416
DOI:10.1071/FP25281