Geographics and bacterial networks differently shape the acquired and latent global sewage resistomes.
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| Názov: | Geographics and bacterial networks differently shape the acquired and latent global sewage resistomes. |
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| Autori: | Martiny HM; Research Group for Genomic Epidemiology, Technical University of Denmark, Lyngby, Denmark. pmun@food.dtu.dk., Munk P; Research Group for Genomic Epidemiology, Technical University of Denmark, Lyngby, Denmark. hanmar@food.dtu.dk., Fuschi A; Department of Physics and Astronomy (DIFA), University of Bologna, Bologna, Italy., Becsei Á; Department of Physics of Complex Systems, ELTE Eötvös Loránd University, Budapest, Hungary., Pyrounakis N; Research Group for Genomic Epidemiology, Technical University of Denmark, Lyngby, Denmark., Brinch C; Research Group for Genomic Epidemiology, Technical University of Denmark, Lyngby, Denmark., Larsson DGJ; Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, and Centre for Antibiotic Resistance Research in Gothenburg, Gothenburg, Sweden., Koopmans M; Erasmus Medical Centre, Rotterdam, The Netherlands., Remondini D; Department of Physics and Astronomy (DIFA), University of Bologna, Bologna, Italy., Csabai I; Department of Physics of Complex Systems, ELTE Eötvös Loránd University, Budapest, Hungary., Aarestrup FM; Research Group for Genomic Epidemiology, Technical University of Denmark, Lyngby, Denmark. |
| Korporácia: | Global Sewage Consortium |
| Zdroj: | Nature communications [Nat Commun] 2025 Nov 21; Vol. 16 (1), pp. 10278. Date of Electronic Publication: 2025 Nov 21. |
| Spôsob vydávania: | Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [London] : Nature Pub. Group |
| Výrazy zo slovníka MeSH: | Sewage*/microbiology , Bacteria*/genetics , Bacteria*/drug effects , Bacteria*/classification , Bacteria*/isolation & purification , Drug Resistance, Bacterial*/genetics, Metagenomics ; Genes, Bacterial/genetics ; North America ; Geography ; Cities ; Anti-Bacterial Agents/pharmacology |
| Abstrakt: | Competing Interests: Competing interests: The authors declare no competing interests. Antimicrobial resistance genes (ARGs) have rapidly emerged and spread globally, but the pathways driving their spread remain poorly understood. We analyzed 1240 sewage samples from 351 cities across 111 countries, comparing ARGs known to be mobilized with those identified through functional metagenomics (FG). FG ARGs showed stronger associations with bacterial taxa than the acquired ARGs. Network analyses further confirmed this and showed potential for source attribution of both known and novel ARGs. The FG resistome was more evenly dispersed globally, whereas the acquired resistome followed distinct geographical patterns. City-wise distance-decay analyses revealed that the FG ARGs showed significant decay within countries but not across regions or globally. In contrast, acquired ARGs showed decay at both national and regional scales. At the variant level, both ARG groups had significant national and regional distance-decay effects, but only FG ARGs at a global scale. Additionally, we observed stronger distance effects in Sub-Saharan Africa and East Asia compared to North America. Our findings suggest that differential selection and niche competition, rather than dispersal, shape the global resistome patterns. A limited number of bacterial taxa may act as reservoirs of latent FG ARGs, highlighting the need of targeted surveillance to mitigate future resistance threats. (© 2025. The Author(s).) |
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| Grant Information: | NNF16OC0021856: Global Surveillance of Antimicrobial Resistance Novo Nordisk Fonden (Novo Nordisk Foundation); 874735 EC | EU Framework Programme for Research and Innovation H2020 | H2020 Excellent Science (H2020 Priority Excellent Science) |
| Contributed Indexing: | Investigator: DG Joakim Larsson; M Koopmans; N Zaatout; C Rees; G Jiang; J Shi; B Benka; F Allerberger; S Koeberl-Jelovcan; KH Abdulla; AB Thani; A Begum; C Worrell; TV Dougnon; F Soria; N Mazalica; T Rahube; AF Martins; CA Tagliati; LCR Souza; A Nzouankeu; MA Rehman; J Gauthier; RC Levesque; SD Workman; C Yost; AA Nakashima; A Opazo; G Gonzales; Y Yu; P Donado-Godoy; T Youssouf; PC Rivera-Navarro; M Jergovic; J Hrenovic; R Karpiskova; JK Coulibaly; W Calero-Cáceres; M Abouelnaga; AM Hokajärvi; A Heikinheimo; SL Guyader; A Nitsche; A Brinkmann; S Schubert; T Berendonk; U Klümper; E Bonah; SA Sefa; A Camilleri; CK Setsoafia Saba; VB Sedor; K Mellou; T Sideroglou; C Kotzamanidis; JB Henriksen; M Møller; TR Thorsteinsdottir; AAM Hatha; S Mohammad; B Shamurad; HA Faraj; D Morris; L O'Connor; J Moran-Gilad; R Orletti; G Rosa; M Iaconelli; A Battisti; L Decastelli; M Napoleoni; A Cesare; G Corno; J Avsejenko; GM Matar; C Penny; LH Samison; DL Banda; H Rajandas; S Parimannan; MV Haber; SJ Santchurn; J Carrillo-Reyes; JO Sanchez-Lara; R Arredondo-Hernández; A Vujacic; D Djurovic; P Pal; CJA Campos; I Pattis; S Chambers; GJ Jeunen; C Elikwu; O Osuolale; M Ndahi; O Abiodun-Adewusi; A Ekhosuehi; A Afolayan; K Fashae; MK Aworh; AO Ahmed; IA Raufu; I Odetokun; SM Chiroma; C Mitchell; R Holmstad; N Weiler; D Wasyl; M Zając; E Cardoso; C Manaia; MJ Campos; HG Hur; MJ Song; S Yoon; O Burduniuc; P Hong; V Radosavljevic; AA Cokro; D Gavacová; M Trkov; RH Abubakar; K Keddy; I Martínez-Alcalá; M Cerdà-Cuéllar; A Cañas; F Gonzalez-Candelas; YA Sabiel; TV der Heijden; YP Hong; JJ Medardus; RP Nagassar; C Kürekci; P McNamara; L Wong; E Fuhrmeister; JS Meschke; N Beck; A Maile-Moskowitz; J Thomsen; O Obaidi; B Paterson; A Leonard; L Zhang; K Chau |
| Substance Nomenclature: | 0 (Sewage) 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20251121 Date Completed: 20251121 Latest Revision: 20251124 |
| Update Code: | 20251124 |
| PubMed Central ID: | PMC12639157 |
| DOI: | 10.1038/s41467-025-66070-7 |
| PMID: | 41271719 |
| Databáza: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Competing Interests: Competing interests: The authors declare no competing interests.<br />Antimicrobial resistance genes (ARGs) have rapidly emerged and spread globally, but the pathways driving their spread remain poorly understood. We analyzed 1240 sewage samples from 351 cities across 111 countries, comparing ARGs known to be mobilized with those identified through functional metagenomics (FG). FG ARGs showed stronger associations with bacterial taxa than the acquired ARGs. Network analyses further confirmed this and showed potential for source attribution of both known and novel ARGs. The FG resistome was more evenly dispersed globally, whereas the acquired resistome followed distinct geographical patterns. City-wise distance-decay analyses revealed that the FG ARGs showed significant decay within countries but not across regions or globally. In contrast, acquired ARGs showed decay at both national and regional scales. At the variant level, both ARG groups had significant national and regional distance-decay effects, but only FG ARGs at a global scale. Additionally, we observed stronger distance effects in Sub-Saharan Africa and East Asia compared to North America. Our findings suggest that differential selection and niche competition, rather than dispersal, shape the global resistome patterns. A limited number of bacterial taxa may act as reservoirs of latent FG ARGs, highlighting the need of targeted surveillance to mitigate future resistance threats.<br /> (© 2025. The Author(s).) |
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| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-025-66070-7 |