Functional and genomic characterization of polyethylene degrading yeast Meyerozyma carpophila M6.0.2 isolated from marine plastic debris in East Java Indonesia.
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| Název: | Functional and genomic characterization of polyethylene degrading yeast Meyerozyma carpophila M6.0.2 isolated from marine plastic debris in East Java Indonesia. |
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| Autoři: | Alami NH; Doctoral Program of Biology, School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, 40132, Indonesia.; Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Jl. Raya ITS, Keputih, Sukolilo, Surabaya, 60111, Indonesia., Puteri ZR; Master's Program in Biotechnology, School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, 40132, Indonesia., Esaki T; Department of Earth Resources Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan. esaki.takehiro.684@m.kyushu-u.ac.jp., Sugai Y; Department of Earth Resources Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan., Pratama F; Microbial Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Ganesha No.10, Bandung, 40132, Indonesia., Purwasena IA; Microbial Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Ganesha No.10, Bandung, 40132, Indonesia. isty@itb.ac.id., Aditiawati P; Microbial Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Ganesha No.10, Bandung, 40132, Indonesia. pingkan@itb.ac.id. |
| Zdroj: | Scientific reports [Sci Rep] 2025 Nov 18; Vol. 15 (1), pp. 40437. Date of Electronic Publication: 2025 Nov 18. |
| Způsob vydávání: | Journal Article |
| Jazyk: | English |
| Informace o časopise: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| Výrazy ze slovníku MeSH: | Polyethylene*/metabolism , Plastics*/metabolism , Saccharomycetales*/genetics , Saccharomycetales*/metabolism , Saccharomycetales*/isolation & purification, Indonesia ; Biodegradation, Environmental ; Genome, Fungal ; Whole Genome Sequencing ; Phylogeny ; Genomics |
| Abstrakt: | Polyethylene (PE) is a primary contributor to environmental plastic pollution, posing a critical risk to ecosystems and living organisms. This study screened six yeast strains (BI.4.1.1, K3.1.2, M.5.0.1, M.5.0.3, M.6.0.1, and M.6.0.2) isolated from marine plastic debris in East Java, Indonesia, for their ability to degrade PE. Among these, Meyerozyma carpophila M6.0.2 demonstrated functional characteristics with the highest polyethylene-degrading activity in liquid medium after a 10-day incubation, with a degradation percentage of 0.4923%. This was supported by its biofilm-forming capacity, biosurfactant production, and broad metabolic activity as demonstrated by EcoPlate assays. In addition, Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) analyses of PE films revealed morphological changes and emergence of new peaks, respectively. Whole-genome sequencing (WGS) of strain M6.0.2 was performed using the Illumina NextSeq 2000 platform (PE 150), yielding a genome assembly of 10.34 Mb across 108 contigs with an N50 length of 1.08 Mb. A total of 5,352 putative genes were predicted. These findings highlight Meyerozyma carpophila M6.0.2 as a promising candidate for bioremediation of marine plastic pollution, emphasizing the combination of functional and genomic insights for a better understanding of the mechanism of PE biodegradation. (© 2025. The Author(s).) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Contributed Indexing: | Keywords: Biodegradation; Marine; Plastic; Polyethylene; Waste; Yeast |
| Substance Nomenclature: | 9002-88-4 (Polyethylene) 0 (Plastics) |
| Entry Date(s): | Date Created: 20251118 Date Completed: 20251118 Latest Revision: 20251121 |
| Update Code: | 20251121 |
| PubMed Central ID: | PMC12627587 |
| DOI: | 10.1038/s41598-025-22911-5 |
| PMID: | 41253867 |
| Databáze: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Polyethylene (PE) is a primary contributor to environmental plastic pollution, posing a critical risk to ecosystems and living organisms. This study screened six yeast strains (BI.4.1.1, K3.1.2, M.5.0.1, M.5.0.3, M.6.0.1, and M.6.0.2) isolated from marine plastic debris in East Java, Indonesia, for their ability to degrade PE. Among these, Meyerozyma carpophila M6.0.2 demonstrated functional characteristics with the highest polyethylene-degrading activity in liquid medium after a 10-day incubation, with a degradation percentage of 0.4923%. This was supported by its biofilm-forming capacity, biosurfactant production, and broad metabolic activity as demonstrated by EcoPlate assays. In addition, Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) analyses of PE films revealed morphological changes and emergence of new peaks, respectively. Whole-genome sequencing (WGS) of strain M6.0.2 was performed using the Illumina NextSeq 2000 platform (PE 150), yielding a genome assembly of 10.34 Mb across 108 contigs with an N50 length of 1.08 Mb. A total of 5,352 putative genes were predicted. These findings highlight Meyerozyma carpophila M6.0.2 as a promising candidate for bioremediation of marine plastic pollution, emphasizing the combination of functional and genomic insights for a better understanding of the mechanism of PE biodegradation.<br /> (© 2025. The Author(s).) |
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| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-025-22911-5 |