Amine-functionalized sulfur quantum dots as highly sensitive and specific nano-bioprobes for robust immunofluorescent detection of Staphylococcus aureus.
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| Title: | Amine-functionalized sulfur quantum dots as highly sensitive and specific nano-bioprobes for robust immunofluorescent detection of Staphylococcus aureus. |
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| Authors: | Kumari S; Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, India., Mehta J; Department of Biotechnology, Thapar Institute of Engineering & Technology, Patiala, Punjab, 147004, India., Jain S; Department of Bio-Nanotechnology, College of Biotechnology, CCS Haryana Agricultural University (CCSHAU), Hisar, Haryana, 12500, India., Dilbaghi N; Department of Biotechnology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, 125001, India., Chaudhary GR; Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, India. grc22@pu.ac.in., Kumar S; Department of Physics, Punjab Engineering College (Deemed to be University), Chandigarh, 160012, India. sandeepkumar@pec.edu.in. |
| Source: | Mikrochimica acta [Mikrochim Acta] 2025 Oct 28; Vol. 192 (11), pp. 759. Date of Electronic Publication: 2025 Oct 28. |
| Publication Type: | Journal Article; Validation Study |
| Language: | English |
| Journal Info: | Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7808782 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-5073 (Electronic) Linking ISSN: 00263672 NLM ISO Abbreviation: Mikrochim Acta Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Wien ; New York : Springer-Verlag. |
| MeSH Terms: | Fluorescent Antibody Technique* , Quantum Dots*/chemistry , Staphylococcus aureus*/isolation & purification , Sulfur*/chemistry, Fluorescent Dyes ; Amines/chemistry ; Ethylenediamines |
| Abstract: | Competing Interests: Declarations. Competing interests: The authors declare no competing interests. The growing burden of Staphylococcus aureus (S. aureus) infections, especially those involving antibiotic-resistant strains necessitates the development of advanced detection technologies that are rapid, selective, and adaptable to real-world conditions. In the present work, amine-functionalized sulfur quantum dots (NH (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.) |
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| Grant Information: | (No. SR/PURSE/2024/350 dated 14-10-2024 Department of Science and Technology, Ministry of Science and Technology, India |
| Contributed Indexing: | Keywords: S. aureus; Immunofluorescence; Immunosensor; Nanobioprobe; Quantum dots |
| Substance Nomenclature: | 60V9STC53F (ethylenediamine) 0 (Fluorescent Dyes) 70FD1KFU70 (Sulfur) 0 (Amines) 0 (Ethylenediamines) |
| Entry Date(s): | Date Created: 20251028 Date Completed: 20251128 Latest Revision: 20251128 |
| Update Code: | 20251128 |
| DOI: | 10.1007/s00604-025-07541-y |
| PMID: | 41148360 |
| Database: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | Competing Interests: Declarations. Competing interests: The authors declare no competing interests.<br />The growing burden of Staphylococcus aureus (S. aureus) infections, especially those involving antibiotic-resistant strains necessitates the development of advanced detection technologies that are rapid, selective, and adaptable to real-world conditions. In the present work, amine-functionalized sulfur quantum dots (NH <subscript>2</subscript> -SQDs) conjugated with S. aureus specific antibodies have been explored for highly sensitive and specific fluorescence detection of respective bacteria. To this end, NH <subscript>2</subscript> -SQDs were synthesized using solvothermal methods followed by their conjugation with polyclonal anti-S. aureus antibodies using EDC/NHS chemistry to develop immunofluorescent conjugate probe, NH <subscript>2</subscript> -SQDs-Ab. The so-formed immunofluorescent probe was characterized using different spectroscopic and microscopic techniques to establish the successful synthesis and antibody conjugation. Further, the NH <subscript>2</subscript> -SQDs-Ab was investigated for the precise identification of S. aureus through targeted immunofluorescence. The results demonstrated that NH <subscript>2</subscript> -SQDs-Ab probe is highly specific towards S. aureus even in complicated matrices containing other interfering bacteria. The immunosensor demonstrated a strong and proportional fluorescence response to different S. aureus concentrations, thereby, achieving a detection limit of 10 CFU/mL over a broad linear range of 5× 10 <sup>1</sup> to 5 × 10 <sup>4</sup> CFU/mL, along with outstanding reproducibility (R <sup>2</sup> = 0.9975). Furthermore, the developed immunofluorescent probe also showed outstanding recoveries in real-world samples, which further validates its potential for useful applications in environmental monitoring and food safety.<br /> (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.) |
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| ISSN: | 1436-5073 |
| DOI: | 10.1007/s00604-025-07541-y |