Nucleic Acid Programmable Protein Arrays for Autoantibody Discovery: A Step-by-Step Guide.
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| Název: | Nucleic Acid Programmable Protein Arrays for Autoantibody Discovery: A Step-by-Step Guide. |
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| Autoři: | Warnakulasuriya K; Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, USA., Song L; Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, USA., LaBaer J; Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, USA. jlabaer@asu.edu. |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2980, pp. 387-400. |
| Způsob vydávání: | Journal Article |
| Jazyk: | English |
| Informace o časopise: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| Výrazy ze slovníku MeSH: | Protein Array Analysis*/methods , Autoantibodies*/immunology , Autoantibodies*/analysis , Nucleic Acids*, Humans ; Biomarkers |
| Abstrakt: | This guide outlines a step-by-step methodology for utilizing Nucleic Acid Programmable Protein Arrays (NAPPA) to identify and characterize novel autoantibody biomarkers for specific diseases. The guide starts with a detailed description of the discovery phase design, including target antigens, sample selection, and experimental controls. It further describes the protein microarray fabrication, emphasizing quality control measures to ensure protein expression and reliable array performance. The third section describes the autoantibody discovery and validation process, including sample preparation, data acquisition, and processing. Finally, it offers a range of data analysis strategies for identifying biomarkers using the NAPPA for further investigation. (© 2026. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Rauf F, Anderson KS, LaBaer J (2020) Autoantibodies in early detection of breast cancer. Cancer Epidemiol Biomarkers Prev 29(12):2475–2485. (PMID: 329943417710604) Miersch S, Bian X, Wallstrom G et al (2013) Serological autoantibody profiling of type 1 diabetes by protein arrays. J Proteome 94:486–496. González-González M, Sayagués JM, Muñoz-Bellvís L et al (2021) Tracking the antibody immunome in sporadic colorectal cancer by using antigen self-assembled protein arrays. Cancer 13(11):2718. Bian X, Wasserfall C, Wallstrom G et al (2017) Tracking the antibody immunome in type 1 diabetes using protein arrays. J Proteome Res 16(1):195–203. (PMID: 27690455) Anderson KS, Sibani S, Wallstrom G et al (2011) Protein microarray signature of autoantibody biomarkers for the early detection of breast cancer. J Proteome Res 10(1):85–96. (PMID: 20977275) Madoz-Gurpide J, Kuick R, Wang H et al (2008) Integral protein microarrays for the identification of lung cancer antigens in sera that induce a humoral immune response. Mol Cell Proteomics 7(2):268–281. (PMID: 17916591) Babel I, Barderas R, Diaz-Uriarte R et al (2009) Identification of tumor-associated autoantigens for the diagnosis of colorectal cancer in serum using high density protein microarrays. Mol Cell Proteomics 8(10):2382–2395. (PMID: 196386182758763) Gibson DS, Qiu J, Mendoza EA et al (2012) Circulating and synovial antibody profiling of juvenile arthritis patients by nucleic acid programmable protein arrays. Arthritis Res Ther 14:1–13. Song L, Wiktor P, Qiu J et al (2021) Identification of antibody biomarker using high-density nucleic acid programmable protein array. In Protein microarrays for disease analysis: methods and protocols. p. 47–64. |
| Contributed Indexing: | Keywords: Autoantibody; Biomarker; Discovery and validation; Nucleic Acid Programmable Protein Arrays (NAPPA); Quality control |
| Substance Nomenclature: | 0 (Autoantibodies) 0 (Biomarkers) 0 (Nucleic Acids) |
| Entry Date(s): | Date Created: 20251014 Date Completed: 20251014 Latest Revision: 20251014 |
| Update Code: | 20251014 |
| DOI: | 10.1007/978-1-0716-4832-2_19 |
| PMID: | 41085633 |
| Databáze: | MEDLINE |
Nájsť tento článok vo Web of Science | Abstrakt: | This guide outlines a step-by-step methodology for utilizing Nucleic Acid Programmable Protein Arrays (NAPPA) to identify and characterize novel autoantibody biomarkers for specific diseases. The guide starts with a detailed description of the discovery phase design, including target antigens, sample selection, and experimental controls. It further describes the protein microarray fabrication, emphasizing quality control measures to ensure protein expression and reliable array performance. The third section describes the autoantibody discovery and validation process, including sample preparation, data acquisition, and processing. Finally, it offers a range of data analysis strategies for identifying biomarkers using the NAPPA for further investigation.<br /> (© 2026. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| ISSN: | 1940-6029 |
| DOI: | 10.1007/978-1-0716-4832-2_19 |