Effect of salinity exposure on the antioxidant system of "Taihu No. 3" Macrobrachium Nipponense.
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| Názov: | Effect of salinity exposure on the antioxidant system of "Taihu No. 3" Macrobrachium Nipponense. |
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| Autori: | Zhou R; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China., Xu M; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China., Zhang W; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China., Xiong Y; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China., Fu H; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China., Qiao H; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China., Jiang S; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. jiangsf@ffrc.cn.; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China. jiangsf@ffrc.cn., Jin S; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. jinsb@ffrc.cn.; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China. jinsb@ffrc.cn. |
| Zdroj: | Scientific reports [Sci Rep] 2025 Oct 13; Vol. 15 (1), pp. 35749. Date of Electronic Publication: 2025 Oct 13. |
| Spôsob vydávania: | Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| Výrazy zo slovníka MeSH: | Palaemonidae*/metabolism , Palaemonidae*/physiology , Palaemonidae*/genetics , Salinity* , Antioxidants*/metabolism, Animals ; Gills/metabolism ; Superoxide Dismutase/metabolism ; Hepatopancreas/metabolism ; Oxidative Stress ; Glutathione Peroxidase/metabolism ; Catalase/metabolism ; Malondialdehyde/metabolism ; Glutathione Reductase/metabolism ; Salt Tolerance |
| Abstrakt: | Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Institutional review board: Permissions for the experiments involved in the present study were obtained from the Institutional Animal Care and Use Ethics Committee of the Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences (Wuxi, China) (Authorization NO.20240610006, 10 June 2024). Informed consent: Statement. Not applicable. Conflict of interest: The authors declare no conflicts of interest. China possesses vast saline-alkaline water resources, necessitating their utilization. Macrobrachium nipponense, an economically important freshwater shrimp with notable salinity tolerance, is a candidate for saline aquaculture. This study determined the 96-h LC (© 2025. The Author(s).) |
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| Contributed Indexing: | Keywords: Macrobrachium nipponense; Gills; Hepatopancreas; Immune response; Salinity |
| Substance Nomenclature: | 0 (Antioxidants) EC 1.15.1.1 (Superoxide Dismutase) EC 1.11.1.9 (Glutathione Peroxidase) EC 1.11.1.6 (Catalase) 4Y8F71G49Q (Malondialdehyde) EC 1.8.1.7 (Glutathione Reductase) |
| Entry Date(s): | Date Created: 20251014 Date Completed: 20251014 Latest Revision: 20251017 |
| Update Code: | 20251017 |
| PubMed Central ID: | PMC12518847 |
| DOI: | 10.1038/s41598-025-20622-5 |
| PMID: | 41083711 |
| Databáza: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Institutional review board: Permissions for the experiments involved in the present study were obtained from the Institutional Animal Care and Use Ethics Committee of the Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences (Wuxi, China) (Authorization NO.20240610006, 10 June 2024). Informed consent: Statement. Not applicable. Conflict of interest: The authors declare no conflicts of interest.<br />China possesses vast saline-alkaline water resources, necessitating their utilization. Macrobrachium nipponense, an economically important freshwater shrimp with notable salinity tolerance, is a candidate for saline aquaculture. This study determined the 96-h LC <subscript>50</subscript> of salinity for the genetically improved "Taihu No. 3" strain juveniles across a gradient (0-30 parts per thousand) and investigated associated stress responses. Morphological, physiological, and molecular responses were analyzed via antioxidant enzyme activity (glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA)) and immune gene expression in hepatopancreas and gills. Results showed the 96-h LC <subscript>50</subscript> was 11.841 ppt, significantly lower than wild populations, suggesting enhanced energy allocation towards growth over osmoregulation in "Taihu No. 3". Acute and chronic stress significantly elevated GPx, GR, CAT, SOD activities and MDA levels (P < 0.05), indicating their critical role in mitigating oxidative damage and maintaining homeostasis. Salinity ≥ 10 ppt induced structural damage, including hepatopancreatic basement membrane disruption and gill alterations (enlarged interlamellar spaces, epithelial swelling). Chronic exposure significantly upregulated immune genes (CAT, Mn-SOD, Cu/Zn-SOD) in both tissues (P < 0.05), demonstrating their involvement in saline acclimation. These findings define measurable salinity tolerance limits and elucidate key immune response mechanisms in "Taihu No. 3", providing a scientific basis for its cultivation in saline-alkaline aquaculture.<br /> (© 2025. The Author(s).) |
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| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-025-20622-5 |