Report on Vibrio Species Contamination in Shrimp From the Coast of Pangandaran, West Java, Indonesia.
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| Titel: | Report on Vibrio Species Contamination in Shrimp From the Coast of Pangandaran, West Java, Indonesia. |
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| Autoren: | Herawati T; Master of Marine Conservation, Universitas Padjadjaran, Sumedang, Indonesia.; Department of Marine Science, Universitas Padjadjaran, Sumedang, Indonesia., Rahayu I; Master of Marine Conservation, Universitas Padjadjaran, Sumedang, Indonesia., Aisyah A; Department of Fisheries, Universitas Padjadjaran, Sumedang, Indonesia., Agung MUK; Department of Marine Science, Universitas Padjadjaran, Sumedang, Indonesia., Pasaribu B; Department of Marine Science, Universitas Padjadjaran, Sumedang, Indonesia., Nurhayati A; Department of Fisheries, Universitas Padjadjaran, Sumedang, Indonesia., Ghazali A; Faculty of Science & Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia., Grandiosa R; Department of Fisheries, Universitas Padjadjaran, Sumedang, Indonesia., Faddilah TN; Master of Marine Conservation, Universitas Padjadjaran, Sumedang, Indonesia., Kamiswara R; Master of Marine Conservation, Universitas Padjadjaran, Sumedang, Indonesia. |
| Quelle: | Environmental microbiology reports [Environ Microbiol Rep] 2025 Oct; Vol. 17 (5), pp. e70210. |
| Publikationsart: | Journal Article |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: Society for Applied Microbiology and Blackwell Pub Country of Publication: United States NLM ID: 101499207 Publication Model: Print Cited Medium: Internet ISSN: 1758-2229 (Electronic) Linking ISSN: 17582229 NLM ISO Abbreviation: Environ Microbiol Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [Hoboken, N.J.] : Society for Applied Microbiology and Blackwell Pub., c2009- |
| MeSH-Schlagworte: | Penaeidae*/microbiology , Vibrio*/isolation & purification , Vibrio*/genetics , Vibrio*/classification, Animals ; Indonesia ; Aquaculture ; Bacterial Toxins/genetics ; Vibrio parahaemolyticus/isolation & purification ; Vibrio parahaemolyticus/genetics |
| Abstract: | Bacterial infections in aquatic organisms pose a significant threat to shrimp aquaculture, often leading to production losses. In Pangandaran Regency, early shrimp harvesting is frequently practiced as a response to outbreaks. Previous studies have documented Vibrio and non-Vibrio bacteria in pond water and sediments at five stations, but infections in shrimp tissues remain less explored. This study aimed to identify pathogenic Vibrio species in vannamei shrimp (Litopenaeus vannamei) and wild black tiger shrimp (Penaeus monodon), examine toxin genes, and quantify bacterial abundance. Samples were collected from five stations and analyzed using culture media, Gram staining and Polymerase Chain Reaction (PCR). Vibrio parahaemolyticus and Vibrio alginolyticus were detected at Stations 1 and 5, whereas only V. alginolyticus appeared at Stations 2, 3 and 4. Station 1 showed the highest Vibrio abundance (1.3767 × 10 6 CFU/g), while Station 3 recorded the lowest (0.009 × 10 6 CFU/g), with significant differences (p < 0.05). Non-Vibrio bacteria dominated most stations, except at Station 1 where Vibrio species were predominant. Toxin gene analysis revealed toxR in some isolates, while tdh, trh, pirA and pirB were absent. These findings emphasize the importance of bacterial and genetic monitoring to improve disease surveillance and support sustainable shrimp aquaculture. (© 2025 The Author(s). Environmental Microbiology Reports published by John Wiley & Sons Ltd.) |
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| Grant Information: | 1821/UN6.3.1/PT.00/2024 Universitas Padjadjaran |
| Contributed Indexing: | Keywords: Pangandaran regency; identification; monitoring; pathogenesis; vibrio bacteria |
| Substance Nomenclature: | 0 (Bacterial Toxins) |
| Entry Date(s): | Date Created: 20251010 Date Completed: 20251010 Latest Revision: 20251013 |
| Update Code: | 20251013 |
| PubMed Central ID: | PMC12511563 |
| DOI: | 10.1111/1758-2229.70210 |
| PMID: | 41069183 |
| Datenbank: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | Bacterial infections in aquatic organisms pose a significant threat to shrimp aquaculture, often leading to production losses. In Pangandaran Regency, early shrimp harvesting is frequently practiced as a response to outbreaks. Previous studies have documented Vibrio and non-Vibrio bacteria in pond water and sediments at five stations, but infections in shrimp tissues remain less explored. This study aimed to identify pathogenic Vibrio species in vannamei shrimp (Litopenaeus vannamei) and wild black tiger shrimp (Penaeus monodon), examine toxin genes, and quantify bacterial abundance. Samples were collected from five stations and analyzed using culture media, Gram staining and Polymerase Chain Reaction (PCR). Vibrio parahaemolyticus and Vibrio alginolyticus were detected at Stations 1 and 5, whereas only V. alginolyticus appeared at Stations 2, 3 and 4. Station 1 showed the highest Vibrio abundance (1.3767 × 10 <sup>6</sup> CFU/g), while Station 3 recorded the lowest (0.009 × 10 <sup>6</sup> CFU/g), with significant differences (p < 0.05). Non-Vibrio bacteria dominated most stations, except at Station 1 where Vibrio species were predominant. Toxin gene analysis revealed toxR in some isolates, while tdh, trh, pirA and pirB were absent. These findings emphasize the importance of bacterial and genetic monitoring to improve disease surveillance and support sustainable shrimp aquaculture.<br /> (© 2025 The Author(s). Environmental Microbiology Reports published by John Wiley & Sons Ltd.) |
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| ISSN: | 1758-2229 |
| DOI: | 10.1111/1758-2229.70210 |