Dynamics of peripheral B and γδ T cells during postpartum and pregnancy in dairy cows.
Gespeichert in:
| Titel: | Dynamics of peripheral B and γδ T cells during postpartum and pregnancy in dairy cows. |
|---|---|
| Autoren: | Cainelli S; Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) /Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Esperanza, Argentina., Engler C; Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) /Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Esperanza, Argentina., Peralta MB; Instituto de Ecología Humana y Desarrollo Sustentable. Universidad Católica de Santa Fe (UCSF). Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Argentina., Stassi AF; Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) /Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Esperanza, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral (UNL), Santa Fe, Esperanza, Argentina., Renna MS; Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) /Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Esperanza, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral (UNL), Santa Fe, Esperanza, Argentina., Angeli E; Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) /Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Esperanza, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral (UNL), Santa Fe, Esperanza, Argentina., Ortega HH; Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) /Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Esperanza, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral (UNL), Santa Fe, Esperanza, Argentina., Velázquez M; Instituto de Ecología Humana y Desarrollo Sustentable. Universidad Católica de Santa Fe (UCSF). Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Argentina. Electronic address: melisavel@gmail.com. |
| Quelle: | Animal reproduction science [Anim Reprod Sci] 2025 Nov; Vol. 282, pp. 108012. Date of Electronic Publication: 2025 Oct 04. |
| Publikationsart: | Journal Article |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7807205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2232 (Electronic) Linking ISSN: 03784320 NLM ISO Abbreviation: Anim Reprod Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| MeSH-Schlagworte: | Postpartum Period*/physiology , Postpartum Period*/immunology , B-Lymphocytes*/physiology , Pregnancy, Animal*/immunology, Animals ; Female ; Cattle/physiology ; Cattle/immunology ; Pregnancy ; Progesterone/blood ; Lactation |
| Abstract: | Competing Interests: Declaration of Competing Interest I confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome. The manuscript has been read and approved by all authors. There are no other persons who satisfied the criteria for authorship but are not listed. I further confirm that the order of authors listed in the manuscript has been approved by all of them. I am the sole contact for the Editorial process (including Editorial Manager and direct communications with the office) as Corresponding Author. I am responsible for communicating with the other authors about progress, submissions of revisions and final approval of proofs. During postpartum, dairy cows experience immune modulation not only locally in the uterus, but also in the peripheral immune response in order to achieve implantation and pregnancy. This immune response is directly influenced by the hormonal milieu. The aim of this study was to analyze the number of circulating mononuclear immune cells, such as total T cells, CD4 T cells, CD8 T cells, γδ T cells, B cells, and monocytes, during mid- and late postpartum of dairy cows, taking into account the hormonal milieu. Dairy cows were grouped according to their parturition-conception interval (PCI), as follows: PCI shorter than 100 days (SPCI) and PCI longer than 170 days (LPCI). Percentages of peripheral mononuclear immune cells were evaluated by flow cytometry. Results showed that changes in the counts of total T cells, CD4 T cells and monocytes were not associated with the PCI or hormone concentrations. Differently, CD8 T cells at 45 days in milk were significantly higher in cows from the SPCI group than in those from the LPCI group. In addition, the main findings showed significant associations between the percentages of circulating B cells and γδ T cells and estradiol and progesterone concentrations during lactation. These associations between B cells, γδ T cells and the hormonal milieu may contribute to shorter PCIs through appropriate immunoregulation around conception and during pregnancy. This study provides, for the first time, a comprehensive characterization of mononuclear cell populations beyond 50 days postpartum, revealing their peripheral dynamics during the establishment of receptive reproductive conditions. (Copyright © 2025 Elsevier B.V. All rights reserved.) |
| Contributed Indexing: | Keywords: B cells; Dairy cows; Hormonal milieu; Monocytes; T cells; γδ T cells |
| Substance Nomenclature: | 4G7DS2Q64Y (Progesterone) |
| Entry Date(s): | Date Created: 20251009 Date Completed: 20251113 Latest Revision: 20251114 |
| Update Code: | 20251114 |
| DOI: | 10.1016/j.anireprosci.2025.108012 |
| PMID: | 41067056 |
| Datenbank: | MEDLINE |
Full Text Finder 
Nájsť tento článok vo Web of Science | Abstract: | Competing Interests: Declaration of Competing Interest I confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome. The manuscript has been read and approved by all authors. There are no other persons who satisfied the criteria for authorship but are not listed. I further confirm that the order of authors listed in the manuscript has been approved by all of them. I am the sole contact for the Editorial process (including Editorial Manager and direct communications with the office) as Corresponding Author. I am responsible for communicating with the other authors about progress, submissions of revisions and final approval of proofs.<br />During postpartum, dairy cows experience immune modulation not only locally in the uterus, but also in the peripheral immune response in order to achieve implantation and pregnancy. This immune response is directly influenced by the hormonal milieu. The aim of this study was to analyze the number of circulating mononuclear immune cells, such as total T cells, CD4 T cells, CD8 T cells, γδ T cells, B cells, and monocytes, during mid- and late postpartum of dairy cows, taking into account the hormonal milieu. Dairy cows were grouped according to their parturition-conception interval (PCI), as follows: PCI shorter than 100 days (SPCI) and PCI longer than 170 days (LPCI). Percentages of peripheral mononuclear immune cells were evaluated by flow cytometry. Results showed that changes in the counts of total T cells, CD4 T cells and monocytes were not associated with the PCI or hormone concentrations. Differently, CD8 T cells at 45 days in milk were significantly higher in cows from the SPCI group than in those from the LPCI group. In addition, the main findings showed significant associations between the percentages of circulating B cells and γδ T cells and estradiol and progesterone concentrations during lactation. These associations between B cells, γδ T cells and the hormonal milieu may contribute to shorter PCIs through appropriate immunoregulation around conception and during pregnancy. This study provides, for the first time, a comprehensive characterization of mononuclear cell populations beyond 50 days postpartum, revealing their peripheral dynamics during the establishment of receptive reproductive conditions.<br /> (Copyright © 2025 Elsevier B.V. All rights reserved.) |
|---|---|
| ISSN: | 1873-2232 |
| DOI: | 10.1016/j.anireprosci.2025.108012 |