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Rapid sonication-assisted whole tissue clearing and immunostaining.

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Názov: Rapid sonication-assisted whole tissue clearing and immunostaining.
Autori: Cheung HPH; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Sha Tin, New Territories, Hong Kong SAR, China.; Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, Sha Tin, New Territories, Hong Kong SAR, China.; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Shatin, New Territories, Hong Kong SAR, China., Lauwers M; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Shatin, New Territories, Hong Kong SAR, China., Wang Z; Research Center of Biomass 3D Printing Materials, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China., Wang J; College of Materials Science, Hunan University, Changsha, 410082, China., Ning C; School of Materials Science and Engineering, National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510641, China., Ker DFE; Department of Biomedical Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR, China., Wang DM; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Sha Tin, New Territories, Hong Kong SAR, China. wangmd@cuhk.edu.hk.; Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, Sha Tin, New Territories, Hong Kong SAR, China. wangmd@cuhk.edu.hk.; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Shatin, New Territories, Hong Kong SAR, China. wangmd@cuhk.edu.hk.; Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Sha Tin, New Territories, Hong Kong SAR, China. wangmd@cuhk.edu.hk.
Zdroj: Scientific reports [Sci Rep] 2025 Oct 08; Vol. 15 (1), pp. 35101. Date of Electronic Publication: 2025 Oct 08.
Spôsob vydávania: Journal Article
Jazyk: English
Informácie o časopise: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Imprint Name(s): Original Publication: London : Nature Publishing Group, copyright 2011-
Výrazy zo slovníka MeSH: Sonication*/methods , Fluorescent Antibody Technique*/methods, Animals ; Mice ; Rats ; Tendons ; Spleen
Abstrakt: Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
High-resolution mapping of three-dimensional structures in biological tissues is essential for understanding various biological processes. However, the optical heterogeneity of these tissues, marked by varying optical properties, causes light scattering and absorption, complicating imaging. Current tissue clearing methods can take between 48 h and 32 days, and the limited diffusion depth of fluorescent probes restricts whole-tissue imaging. This study introduces an innovative Sonication-Assisted Tissue Clearing and Immunofluorescent Staining method (SoniC/S), which combines low-frequency ultrasound with a commercial chemical clearing kit and iDISCO staining techniques. When tested on the soft tissue of mouse muscle, the dense collagenous tissue of rat tendon, and the heme-rich tissue of mouse spleen, SoniC/S achieved complete clearing in just 36 h and uniform labeling in 15 h. Overall, SoniC/S provides a rapid and effective approach for tissue clearing and deep immunostaining, facilitating high-resolution volumetric imaging in biological research.
(© 2025. The Author(s).)
References: Ultrason Sonochem. 2021 Aug;76:105653. (PMID: 34198127)
Lasers Surg Med. 2025 Sep;57(7):625-636. (PMID: 40735876)
Cell. 2015 Dec 3;163(6):1500-14. (PMID: 26638076)
Nat Protoc. 2021 Jan;16(1):239-262. (PMID: 33247285)
Nat Methods. 2020 Jun;17(6):609-613. (PMID: 32424271)
iScience. 2021 Apr 01;24(4):102387. (PMID: 33981974)
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7321-E7330. (PMID: 28808033)
Sci Adv. 2020 May 29;6(22):eaba0365. (PMID: 32523996)
Nat Methods. 2022 Sep;19(9):1137-1146. (PMID: 36050489)
Prog Histochem Cytochem. 2016 Aug;51(2):9-23. (PMID: 27142295)
Sci Rep. 2015 May 27;5:10640. (PMID: 26013317)
Nat Biotechnol. 2018 Dec 17;:. (PMID: 30556815)
Adv Mater. 2025 Jan;37(2):e2313722. (PMID: 39417770)
Laser Photon Rev. 2013 Sep;7(5):732-757. (PMID: 24348874)
Nat Protoc. 2012 Nov;7(11):1983-95. (PMID: 23060243)
Int J Radiat Oncol Biol Phys. 1995 Sep 30;33(2):323-8. (PMID: 7673019)
Exp Ther Med. 2011 Sep;2(5):849-852. (PMID: 22977587)
Cell Chem Biol. 2016 Jan 21;23(1):137-157. (PMID: 26933741)
Biol Proced Online. 2024 Oct 4;26(1):31. (PMID: 39367314)
Foods. 2023 Jan 11;12(2):. (PMID: 36673438)
PLoS One. 2016 Mar 01;11(3):e0150268. (PMID: 26930293)
J Biomed Opt. 1997 Oct;2(4):401-17. (PMID: 23014964)
Nat Neurosci. 2011 Aug 30;14(11):1481-8. (PMID: 21878933)
Lasers Surg Med. 2024 Dec;56(10):829-835. (PMID: 39593274)
J Biomed Opt. 2008 Mar-Apr;13(2):021113. (PMID: 18465962)
Cell Rep Methods. 2023 Apr 20;3(5):100458. (PMID: 37323568)
Nat Neurosci. 2013 Aug;16(8):1154-61. (PMID: 23792946)
Sci Rep. 2020 Nov 17;10(1):20006. (PMID: 33203925)
Nature. 2013 May 16;497(7449):332-7. (PMID: 23575631)
Sci Rep. 2017 Sep 22;7(1):12218. (PMID: 28939860)
Nat Protoc. 2014 Jul;9(7):1682-97. (PMID: 24945384)
Nat Methods. 2007 Apr;4(4):331-6. (PMID: 17384643)
Proc Natl Acad Sci U S A. 2015 Nov 17;112(46):E6274-83. (PMID: 26578787)
Sci Rep. 2018 Aug 24;8(1):12815. (PMID: 30143733)
Nat Biotechnol. 2001 Apr;19(4):316-7. (PMID: 11283581)
iScience. 2020 Aug 21;23(8):101432. (PMID: 32805648)
Int J Mol Sci. 2023 Oct 14;24(20):. (PMID: 37894875)
Development. 2013 Mar;140(6):1364-8. (PMID: 23444362)
Sci Adv. 2019 Jan 11;5(1):eaau8355. (PMID: 30746463)
Annu Rev Cell Dev Biol. 2016 Oct 6;32:713-741. (PMID: 27298088)
Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
Front Neuroanat. 2015 Feb 24;9:19. (PMID: 25759641)
Cell Rep. 2016 Mar 22;14(11):2718-32. (PMID: 26972009)
Ann Transl Med. 2020 Mar;8(6):298. (PMID: 32355742)
Bioact Mater. 2024 Mar 05;36:221-237. (PMID: 38481565)
Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):E1149-58. (PMID: 24616509)
J Biomech Eng. 2004 Feb;126(1):104-10. (PMID: 15171136)
Sci Rep. 2016 Jan 11;6:18631. (PMID: 26750588)
Cell. 2014 Apr 24;157(3):726-39. (PMID: 24746791)
Nat Neurosci. 2019 Feb;22(2):317-327. (PMID: 30598527)
Expert Opin Drug Deliv. 2004 Nov;1(1):37-56. (PMID: 16296719)
Lasers Surg Med. 1999;24(2):133-41. (PMID: 10100651)
Skelet Muscle. 2018 Jul 19;8(1):21. (PMID: 30025544)
Soft Matter. 2019 Apr 3;15(14):3055-3064. (PMID: 30912548)
Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 15;197:216-229. (PMID: 29433855)
Sci Rep. 2019 Oct 31;9(1):15791. (PMID: 31673001)
Cell Res. 2018 Aug;28(8):803-818. (PMID: 29844583)
Nat Rev Cancer. 2021 Nov;21(11):718-730. (PMID: 34331034)
Front Cell Neurosci. 2019 Feb 27;13:49. (PMID: 30873005)
J Anat. 2021 Feb;238(2):489-507. (PMID: 32939792)
ACS Omega. 2023 Jan 06;8(2):1739-1758. (PMID: 36687066)
PLoS One. 2015 Jan 12;10(1):e0116662. (PMID: 25581165)
J Theor Biol. 1999 Jul 21;199(2):181-98. (PMID: 10395813)
Nat Methods. 2016 Oct;13(10):859-67. (PMID: 27548807)
J Biophotonics. 2019 Feb;12(2):e201800134. (PMID: 30318789)
Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11480-11489. (PMID: 31101714)
iScience. 2021 Feb 12;24(3):102178. (PMID: 33718830)
J Biophotonics. 2019 Aug;12(8):e201800368. (PMID: 30932329)
Commun Biol. 2022 May 9;5(1):431. (PMID: 35534680)
Sci Rep. 2017 Aug 29;7(1):9895. (PMID: 28852046)
Cell. 2014 Nov 6;159(4):896-910. (PMID: 25417164)
Biol Proced Online. 2020 Jul 01;22:13. (PMID: 32624710)
Cell. 2014 Aug 14;158(4):945-958. (PMID: 25088144)
BMC Dev Biol. 2014 Dec 21;14:48. (PMID: 25528649)
Nat Methods. 2013 Jun;10(6):508-13. (PMID: 23722210)
Lasers Surg Med. 2011 Sep;43(8):814-23. (PMID: 21956629)
Nat Methods. 2022 Apr;19(4):479-485. (PMID: 35347322)
Commun Biol. 2021 Mar 5;4(1):294. (PMID: 33674758)
Cell J. 2018 Jul;20(2):132-137. (PMID: 29633589)
Sci Rep. 2018 Jan 31;8(1):1964. (PMID: 29386656)
Grant Information: ITS-020-23MX Innovation and Technology Commission of the Hong Kong SAR Government; GRF 14118620 Research Grants Council of Hong Kong SAR; N_CUHK409/23 The National Natural Science Foundation of China /Research Grants Council Joint Research Scheme
Contributed Indexing: Keywords: Optical imaging; Sonication; Tissue clearing; Whole-tissue immunostaining
Entry Date(s): Date Created: 20251008 Date Completed: 20251008 Latest Revision: 20251011
Update Code: 20251011
PubMed Central ID: PMC12508232
DOI: 10.1038/s41598-025-18928-5
PMID: 41062556
Databáza: MEDLINE
Popis
Abstrakt:Competing Interests: Declarations. Competing interests: The authors declare no competing interests.<br />High-resolution mapping of three-dimensional structures in biological tissues is essential for understanding various biological processes. However, the optical heterogeneity of these tissues, marked by varying optical properties, causes light scattering and absorption, complicating imaging. Current tissue clearing methods can take between 48 h and 32 days, and the limited diffusion depth of fluorescent probes restricts whole-tissue imaging. This study introduces an innovative Sonication-Assisted Tissue Clearing and Immunofluorescent Staining method (SoniC/S), which combines low-frequency ultrasound with a commercial chemical clearing kit and iDISCO staining techniques. When tested on the soft tissue of mouse muscle, the dense collagenous tissue of rat tendon, and the heme-rich tissue of mouse spleen, SoniC/S achieved complete clearing in just 36 h and uniform labeling in 15 h. Overall, SoniC/S provides a rapid and effective approach for tissue clearing and deep immunostaining, facilitating high-resolution volumetric imaging in biological research.<br /> (© 2025. The Author(s).)
ISSN:2045-2322
DOI:10.1038/s41598-025-18928-5