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Metagenomic insights to effective elimination of resistomes in food waste composting by lime addition.

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Název: Metagenomic insights to effective elimination of resistomes in food waste composting by lime addition.
Autoři: Zhang L; Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; Key Laboratory of Southwestern Organic Recycling Engineering Center, Dali Bai Autonomous Prefecture, Yunnan 671000, China., Gao X; Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China., Li G; Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China., Xu Z; Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China. Electronic address: xuzc@cau.edu.cn., Luo W; Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China. Electronic address: luowenhai@cau.edu.cn.
Zdroj: Journal of hazardous materials [J Hazard Mater] 2025 Nov 05; Vol. 499, pp. 140065. Date of Electronic Publication: 2025 Oct 06.
Způsob vydávání: Journal Article
Jazyk: English
Informace o časopise: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam : Elsevier,
Výrazy ze slovníku MeSH: Calcium Compounds*/pharmacology , Calcium Compounds*/chemistry , Oxides*/pharmacology , Oxides*/chemistry , Composting*/methods , Drug Resistance, Bacterial*/genetics, Metagenomics ; Bacteria/genetics ; Bacteria/drug effects ; Gene Transfer, Horizontal ; Food ; Metals, Heavy ; Genes, Bacterial ; Anti-Bacterial Agents/pharmacology ; Food Loss and Waste
Abstrakt: Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Food waste contains abundant resistomes, including antibiotic and heavy metal resistance genes (ARGs and MRGs), which pose risks to the environment and human health. Composting can be used for food waste treatment, but it fails to effectively eliminate these resistomes. Thus, this study investigated the performance of lime to regulate the dynamics and mobility of ARGs and MRGs in food waste composting by metagenomics. Genome-resolved analysis was further conducted to identify the ARGs and MRGs hosts and their horizontal gene transfer (HGT) events. Results showed that lime addition at 1 % (wet weight) could significantly promote temperature and pH increase to sterilize hosts, particularly pathogen bacteria (e.g. Acinetobacter johnsonii and Enterobacter cloacae), thus reducing the abundance of resistomes by more than 57.1 %. This sterilization notably reduced the number of mobile ARGs and MRGs driven by mobile genetic elements (MGEs). The contribution of MGEs located on chromosomal sequences to horizontally transfer ARGs and MRGs was significantly higher than that on mobilizable plasmids. Further analysis indicated that the reduced resistomes by lime was mainly attributed to effective sterilization of hosts rather than decreased HGT diversity. Thus, this study provides valuable insights into use lime as a low-cost control of resistomes in waste recycling.
(Copyright © 2025 Elsevier B.V. All rights reserved.)
Contributed Indexing: Keywords: Antibiotic resistance genes; Food waste composting; Heavy metal resistance genes; Horizontal gene transfer; Mobile genetic elements
Substance Nomenclature: 0 (Calcium Compounds)
0 (Oxides)
C7X2M0VVNH (lime)
0 (Metals, Heavy)
0 (Anti-Bacterial Agents)
0 (Food Loss and Waste)
Entry Date(s): Date Created: 20251008 Date Completed: 20251122 Latest Revision: 20251122
Update Code: 20251122
DOI: 10.1016/j.jhazmat.2025.140065
PMID: 41061540
Databáze: MEDLINE
Popis
Abstrakt:Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br />Food waste contains abundant resistomes, including antibiotic and heavy metal resistance genes (ARGs and MRGs), which pose risks to the environment and human health. Composting can be used for food waste treatment, but it fails to effectively eliminate these resistomes. Thus, this study investigated the performance of lime to regulate the dynamics and mobility of ARGs and MRGs in food waste composting by metagenomics. Genome-resolved analysis was further conducted to identify the ARGs and MRGs hosts and their horizontal gene transfer (HGT) events. Results showed that lime addition at 1 % (wet weight) could significantly promote temperature and pH increase to sterilize hosts, particularly pathogen bacteria (e.g. Acinetobacter johnsonii and Enterobacter cloacae), thus reducing the abundance of resistomes by more than 57.1 %. This sterilization notably reduced the number of mobile ARGs and MRGs driven by mobile genetic elements (MGEs). The contribution of MGEs located on chromosomal sequences to horizontally transfer ARGs and MRGs was significantly higher than that on mobilizable plasmids. Further analysis indicated that the reduced resistomes by lime was mainly attributed to effective sterilization of hosts rather than decreased HGT diversity. Thus, this study provides valuable insights into use lime as a low-cost control of resistomes in waste recycling.<br /> (Copyright © 2025 Elsevier B.V. All rights reserved.)
ISSN:1873-3336
DOI:10.1016/j.jhazmat.2025.140065