Immunofluorescence Staining and Microscopic Imaging of Plant Nuclei for Epigenetic Modifications.
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| Title: | Immunofluorescence Staining and Microscopic Imaging of Plant Nuclei for Epigenetic Modifications. |
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| Authors: | Gandhivel VH; National Centre for Biological Sciences, TIFR, Bangalore, India.; Donald Danforth Plant Science Center, St. Louis, MO, USA., Raju S; National Centre for Biological Sciences, TIFR, Bangalore, India.; SASTRA University, Thanjavur, India., Shivaprasad PV; National Centre for Biological Sciences, TIFR, Bangalore, India. shivaprasad@ncbs.res.in. |
| Source: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2988, pp. 83-92. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| MeSH Terms: | Epigenesis, Genetic* , Cell Nucleus*/metabolism , Cell Nucleus*/genetics , Fluorescent Antibody Technique*/methods , Plants*/genetics , Plants*/metabolism, Protein Processing, Post-Translational ; DNA Methylation ; Chromatin/metabolism ; Chromatin/genetics ; Histones/metabolism ; Microscopy, Fluorescence/methods ; Epigenomics/methods |
| Abstract: | Histone posttranslational modifications (PTMs) and DNA methylation are the predominant epigenetic modifications on the chromatin that regulate gene expression. These modifications can be spatially resolved using microscopic examination of the nuclei with the help of commercially available antibodies. Here, we describe a detailed method to obtain intact nuclei from plant tissues and reproducibly immunostain the nuclei for specific chromatin marks and microscopic examination. This method can be readily extended to multiple plant species as the antibodies are raised against conserved epigenetic marks. (© 2026. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Candela-Ferre J, Diego-Martin B, Pérez-Alemany J, Gallego-Bartolomé J (2024) Mind the gap: epigenetic regulation of chromatin accessibility in plants. Plant Physiol 194:1998–2016. (PMID: 10.1093/plphys/kiae0243823630310980423) Foroozani M, Holder DH, Deal RB (2022) Histone variants in the specialization of plant chromatin. Annu Rev Plant Biol 73:149–172. (PMID: 10.1146/annurev-arplant-070221-050044351677589133179) Kawashima T, Berger F (2014) Epigenetic reprogramming in plant sexual reproduction. Nat Rev Genet 15:613–624. (PMID: 10.1038/nrg368525048170) Gehring M (2019) Epigenetic dynamics during flowering plant reproduction: evidence for reprogramming? New Phytol 224:91–96. (PMID: 10.1111/nph.15856310021746711810) Zhang W, Cheng L, Li K et al (2024) Evolutional heterochromatin condensation delineates chromocenter formation and retrotransposon silencing in plants. Nat Plants 10:1215–1230. (PMID: 10.1038/s41477-024-01746-439014153) |
| Contributed Indexing: | Keywords: Chromatin marks; Epigenetics; Histone marks; Immunofluorescence; Nuclear staining |
| Substance Nomenclature: | 0 (Chromatin) 0 (Histones) |
| Entry Date(s): | Date Created: 20251006 Date Completed: 20251006 Latest Revision: 20251006 |
| Update Code: | 20251006 |
| DOI: | 10.1007/978-1-0716-4981-7_8 |
| PMID: | 41051668 |
| Database: | MEDLINE |
Nájsť tento článok vo Web of Science | Abstract: | Histone posttranslational modifications (PTMs) and DNA methylation are the predominant epigenetic modifications on the chromatin that regulate gene expression. These modifications can be spatially resolved using microscopic examination of the nuclei with the help of commercially available antibodies. Here, we describe a detailed method to obtain intact nuclei from plant tissues and reproducibly immunostain the nuclei for specific chromatin marks and microscopic examination. This method can be readily extended to multiple plant species as the antibodies are raised against conserved epigenetic marks.<br /> (© 2026. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| ISSN: | 1940-6029 |
| DOI: | 10.1007/978-1-0716-4981-7_8 |