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Treeshrews as a potential reservoir: First detection of dengue virus serotype 2 in Malaysian treeshrew faeces.

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Název: Treeshrews as a potential reservoir: First detection of dengue virus serotype 2 in Malaysian treeshrew faeces.
Autoři: Siew ZY; School of Pharmacy, University of Nottingham Malaysia, Jalan Broga, Semenyih, Selangor 43500, Malaysia. Electronic address: siewzy0@gmail.com., Tang CZ; School of Pharmacy, University of Nottingham Malaysia, Jalan Broga, Semenyih, Selangor 43500, Malaysia; Centre for Environmental and Population Health, Institute for Research, Development and Innovation (IRDI), IMU University, Kuala Lumpur, Malaysia., Musa SNA; School of Pharmacy, University of Nottingham Malaysia, Jalan Broga, Semenyih, Selangor 43500, Malaysia., Seow I; School of Pharmacy, University of Nottingham Malaysia, Jalan Broga, Semenyih, Selangor 43500, Malaysia., Johari NA; Centre for Environmental and Population Health, Institute for Research, Development and Innovation (IRDI), IMU University, Kuala Lumpur, Malaysia., Leong PP; M. Kandiah Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Jalan Sungai Long, Bandar Sungai Long, 43000 Kajang, Selangor, Malaysia., Wong ST; Department of Pathology and Pharmacology, School of Medicine, IMU University, Kuala Lumpur, Malaysia., Voon K; School of Pharmacy, University of Nottingham Malaysia, Jalan Broga, Semenyih, Selangor 43500, Malaysia. Electronic address: kenny.voon@nottingham.edu.my.
Zdroj: Journal of virological methods [J Virol Methods] 2026 Jan; Vol. 339, pp. 115256. Date of Electronic Publication: 2025 Aug 31.
Způsob vydávání: Journal Article
Jazyk: English
Informace o časopise: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
Imprint Name(s): Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
Výrazy ze slovníku MeSH: Dengue Virus*/isolation & purification , Dengue Virus*/genetics , Dengue Virus*/classification , Feces*/virology , Disease Reservoirs*/virology , Tupaiidae*/virology , Dengue*/virology , Dengue*/transmission, Animals ; Malaysia ; Serogroup ; RNA, Viral/genetics ; Chlorocebus aethiops ; Vero Cells ; Humans ; Genotype
Abstrakt: Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Arboviruses are transmitted to humans and animals by arthropods and can be fatal. Dengue fever remains a major mosquito-borne disease in tropical regions, primarily spread by Aedes aegypti and Aedes albopictus. Despite vector control and vaccine efforts, dengue virus (DENV) continues to pose serious public health challenges in Malaysia. While non-human primates are known reservoirs in sylvatic cycles, the role of other mammals like treeshrews (Tupaia glis) is poorly understood. This study screened wild treeshrews in suburban Semenyih, Malaysia, for DENV and its serotype. From 2023-2024, fecal and urine samples were collected and pooled for molecular screening. Viral RNA was extracted and tested via RT-PCR targeting the Capsid-Premembrane (C-prM) region. Of 11 samples, three (27.3 %) were positive for DENV-2. Sequence analysis revealed the cosmopolitan genotype II, typically linked to human transmission, rather than sylvatic strains. Virus isolation in Vero and C6/36 cells showed cytopathic effects, though contamination hampered results. These findings suggest treeshrews may serve as incidental reservoirs or amplifying hosts of DENV-2, highlighting the need for wildlife surveillance to better understand dengue transmission and guide public health responses.
(Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.)
Contributed Indexing: Keywords: Arbovirus; Dengue virus; Pathogen; Reservoir; Surveillance; Treeshrew; Tupaia glis; Zoonotic virus
Substance Nomenclature: 0 (RNA, Viral)
Entry Date(s): Date Created: 20250902 Date Completed: 20251018 Latest Revision: 20251018
Update Code: 20251019
DOI: 10.1016/j.jviromet.2025.115256
PMID: 40897249
Databáze: MEDLINE
Popis
Abstrakt:Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br />Arboviruses are transmitted to humans and animals by arthropods and can be fatal. Dengue fever remains a major mosquito-borne disease in tropical regions, primarily spread by Aedes aegypti and Aedes albopictus. Despite vector control and vaccine efforts, dengue virus (DENV) continues to pose serious public health challenges in Malaysia. While non-human primates are known reservoirs in sylvatic cycles, the role of other mammals like treeshrews (Tupaia glis) is poorly understood. This study screened wild treeshrews in suburban Semenyih, Malaysia, for DENV and its serotype. From 2023-2024, fecal and urine samples were collected and pooled for molecular screening. Viral RNA was extracted and tested via RT-PCR targeting the Capsid-Premembrane (C-prM) region. Of 11 samples, three (27.3 %) were positive for DENV-2. Sequence analysis revealed the cosmopolitan genotype II, typically linked to human transmission, rather than sylvatic strains. Virus isolation in Vero and C6/36 cells showed cytopathic effects, though contamination hampered results. These findings suggest treeshrews may serve as incidental reservoirs or amplifying hosts of DENV-2, highlighting the need for wildlife surveillance to better understand dengue transmission and guide public health responses.<br /> (Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.)
ISSN:1879-0984
DOI:10.1016/j.jviromet.2025.115256