Evaluation of Cytotoxicity and Osteogenic Potential of Strontium Silicate and Calcium Silicate-Based Sealers.
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| Název: | Evaluation of Cytotoxicity and Osteogenic Potential of Strontium Silicate and Calcium Silicate-Based Sealers. |
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| Autoři: | Kwan DCY; Discipline of Endodontology, Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China., Hu M; Discipline of Endodontology, Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China., Liu J; Discipline of Endodontology, Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China., Zhang C; Discipline of Endodontology, Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China., Lee AHC; Discipline of Endodontology, Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China. Electronic address: bollies4@hku.hk., Chang JWW; Discipline of Endodontology, Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China. Electronic address: changww@hku.hk. |
| Zdroj: | International dental journal [Int Dent J] 2025 Dec; Vol. 75 (6), pp. 103869. Date of Electronic Publication: 2025 Sep 01. |
| Způsob vydávání: | Journal Article |
| Jazyk: | English |
| Informace o časopise: | Publisher: Elsevier Country of Publication: England NLM ID: 0374714 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1875-595X (Electronic) Linking ISSN: 00206539 NLM ISO Abbreviation: Int Dent J Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2021- : London : Elsevier Original Publication: London : FDI World Dental |
| Výrazy ze slovníku MeSH: | Calcium Compounds*/toxicity , Calcium Compounds*/pharmacology , Silicates*/toxicity , Silicates*/pharmacology , Osteogenesis*/drug effects , Root Canal Filling Materials*/toxicity , Root Canal Filling Materials*/pharmacology , Osteoblasts*/drug effects , Strontium*/toxicity, Animals ; Mice ; Alkaline Phosphatase/metabolism ; Cell Differentiation/drug effects ; Materials Testing ; Cell Survival/drug effects ; Epoxy Resins/toxicity ; Collagen Type I/metabolism |
| Abstrakt: | Competing Interests: Conflict of interest None disclosed. Objectives: This study aimed to evaluate the cytotoxicity and osteogenic potential of three sealers, including a strontium silicate-based sealer, C-Root SP (C-R), and two calcium silicate-based sealers, iRoot SP (i-R) and AH Plus Bioceramic Sealer (AHPbcs), compared with AH Plus (AHP) on MC3T3-E1 pre-osteoblasts. Materials and Methods: Standardized sealer discs were eluted in a culture medium to assess cytotoxicity using the CCK-8 assay at various dilutions (1:1, 1:2, 1:5, and 1:10). Osteogenic differentiation was evaluated by culturing cells in osteogenic medium supplemented with 1:5 diluted sealer extract. Alkaline phosphatase (ALP) activity was assessed with an ALP assay and staining on days 7 and 14. Mineralized nodule formation was observed using Alizarin Red S staining on day 21. Gene expression of osteogenic markers (ALP, COL1A1, and RUNX2) was examined by RT-qPCR. Differences were analysed using one-way/two-way variance analysis with the Tukey post-hoc test. Statistical significance was established at P < .05. Results: C-R, i-R, and AHPbcs showed significantly higher cell viability than AHP (P < .001). All sealers exhibited cytotoxicity at higher concentrations (1:1 and 1:2 dilutions). ALP activity was significantly lower in cells exposed to AHP compared to cells exposed to C-R, i-R, and AHPbcs (P < .01). Cells exposed to C-R, i-R, and AHPbcs exhibited higher mineralized nodule formation than cells exposed to AHP. C-R, i-R, and AHPbcs enhanced osteogenic differentiation with higher osteogenic gene expression than AHP (P < .001). Conclusions: Within the limitations of the study, C-R, i-R, and AHPbcs were biocompatible with MC3T3-E1 pre-osteoblasts at lower concentrations and were able to enhance their osteogenic potentials. Clinical Relevance: The strontium silicate-based sealer shows a favourable biological response and osteogenic activity in vitro, comparable to calcium silicate-based sealers, indicating its potential for clinical applications. (Copyright © 2025 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Contributed Indexing: | Keywords: Cytotoxicity; Osteoblast; Osteogenic potential; Sealer; Strontium silicate |
| Substance Nomenclature: | 0 (Calcium Compounds) 0 (Silicates) S4255P4G5M (calcium silicate) 0 (Root Canal Filling Materials) YZS2RPE8LE (Strontium) EC 3.1.3.1 (Alkaline Phosphatase) 0 (Epoxy Resins) 0 (Collagen Type I) |
| Entry Date(s): | Date Created: 20250902 Date Completed: 20251113 Latest Revision: 20251113 |
| Update Code: | 20251114 |
| PubMed Central ID: | PMC12423690 |
| DOI: | 10.1016/j.identj.2025.103869 |
| PMID: | 40896885 |
| Databáze: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Competing Interests: Conflict of interest None disclosed.<br />Objectives: This study aimed to evaluate the cytotoxicity and osteogenic potential of three sealers, including a strontium silicate-based sealer, C-Root SP (C-R), and two calcium silicate-based sealers, iRoot SP (i-R) and AH Plus Bioceramic Sealer (AHPbcs), compared with AH Plus (AHP) on MC3T3-E1 pre-osteoblasts.<br />Materials and Methods: Standardized sealer discs were eluted in a culture medium to assess cytotoxicity using the CCK-8 assay at various dilutions (1:1, 1:2, 1:5, and 1:10). Osteogenic differentiation was evaluated by culturing cells in osteogenic medium supplemented with 1:5 diluted sealer extract. Alkaline phosphatase (ALP) activity was assessed with an ALP assay and staining on days 7 and 14. Mineralized nodule formation was observed using Alizarin Red S staining on day 21. Gene expression of osteogenic markers (ALP, COL1A1, and RUNX2) was examined by RT-qPCR. Differences were analysed using one-way/two-way variance analysis with the Tukey post-hoc test. Statistical significance was established at P < .05.<br />Results: C-R, i-R, and AHPbcs showed significantly higher cell viability than AHP (P < .001). All sealers exhibited cytotoxicity at higher concentrations (1:1 and 1:2 dilutions). ALP activity was significantly lower in cells exposed to AHP compared to cells exposed to C-R, i-R, and AHPbcs (P < .01). Cells exposed to C-R, i-R, and AHPbcs exhibited higher mineralized nodule formation than cells exposed to AHP. C-R, i-R, and AHPbcs enhanced osteogenic differentiation with higher osteogenic gene expression than AHP (P < .001).<br />Conclusions: Within the limitations of the study, C-R, i-R, and AHPbcs were biocompatible with MC3T3-E1 pre-osteoblasts at lower concentrations and were able to enhance their osteogenic potentials.<br />Clinical Relevance: The strontium silicate-based sealer shows a favourable biological response and osteogenic activity in vitro, comparable to calcium silicate-based sealers, indicating its potential for clinical applications.<br /> (Copyright © 2025 The Authors. Published by Elsevier Inc. All rights reserved.) |
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| ISSN: | 1875-595X |
| DOI: | 10.1016/j.identj.2025.103869 |