Molecular Mechanism of a Conserved Odorant Receptor in Detecting a Repellent Signal in the Rice Moth Corcyra cephalonica .
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| Názov: | Molecular Mechanism of a Conserved Odorant Receptor in Detecting a Repellent Signal in the Rice Moth Corcyra cephalonica . |
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| Autori: | Zhu X; Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China.; Key Laboratory of Vegetation Ecology, Ministry of Education, Northeast Normal University, Changchun 130024, China.; Jilin Provincial Engineering Laboratory of Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun 130024, China., Zhou X; Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China.; Key Laboratory of Vegetation Ecology, Ministry of Education, Northeast Normal University, Changchun 130024, China.; Jilin Provincial Engineering Laboratory of Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun 130024, China., Fei S; Wageningen University & Research Plant Sciences, Wageningen 6708 HB, Netherlands.; Engineering Research Center of Natural Enemies, Institute of Biological Control, Jilin Agricultural University, Changchun 130118, China., Wang Z; Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China.; Key Laboratory of Vegetation Ecology, Ministry of Education, Northeast Normal University, Changchun 130024, China.; Jilin Provincial Engineering Laboratory of Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun 130024, China., Du W; Engineering Research Center of Natural Enemies, Institute of Biological Control, Jilin Agricultural University, Changchun 130118, China., Zhang J; Engineering Research Center of Natural Enemies, Institute of Biological Control, Jilin Agricultural University, Changchun 130118, China.; Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Changchun 130024, China., Ren B; Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China.; Key Laboratory of Vegetation Ecology, Ministry of Education, Northeast Normal University, Changchun 130024, China.; Jilin Provincial Engineering Laboratory of Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun 130024, China.; Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Changchun 130024, China., Chen Q; Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China.; Key Laboratory of Vegetation Ecology, Ministry of Education, Northeast Normal University, Changchun 130024, China.; Jilin Provincial Engineering Laboratory of Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun 130024, China.; Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Changchun 130024, China. |
| Zdroj: | Journal of agricultural and food chemistry [J Agric Food Chem] 2025 Sep 10; Vol. 73 (36), pp. 22283-22294. Date of Electronic Publication: 2025 Aug 31. |
| Spôsob vydávania: | Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, American Chemical Society. |
| Výrazy zo slovníka MeSH: | Receptors, Odorant*/genetics , Receptors, Odorant*/metabolism , Receptors, Odorant*/chemistry , Moths*/genetics , Moths*/metabolism , Moths*/chemistry , Moths*/drug effects , Insect Repellents*/metabolism , Insect Repellents*/chemistry , Insect Proteins*/genetics , Insect Proteins*/metabolism , Insect Proteins*/chemistry, Animals ; Phenylethyl Alcohol/metabolism ; Phylogeny |
| Abstrakt: | The rice moth Corcyra cephalonica , a highly destructive pest found worldwide, with a wide-ranging diet and robust reproduction, severely damages various stored foods. A sensitive olfactory system is essential for insect behavioral perception, odorant receptors (ORs) play a crucial role in detecting volatile chemical cues in the living environment of insects. However, the reports on the identification and functional analyses of ORs in C. cephalonica are currently lacking. In this study, we identified CcepOR13, which clusters into the conserved lepidopteran OR42 ortholog family. TEVC recordings revealed its conserved function, and phenylacetaldehyde, 2-phenylethanol, cinnamaldehyde, and pentanal were identified as its main ligands. Behavioral experiments revealed that among these four ligands, C. cephalonica exhibited avoidance behavior only in response to 2-phenylethanol, and after dsRNA-CcepOR13 treatment, its electrophysiological and avoidance responses to 2-phenylethanol were significantly reduced. Our findings indicate that CcepOR13 is essential for 2-phenylethanol recognition by C. cephalonica , suggesting that the development of repellents for biological control is warranted. |
| Contributed Indexing: | Keywords: 2-phenylethanol; Corcyra cephalonica; RNA interference; Xenopus oocytes; conserved odorant receptor |
| Substance Nomenclature: | 0 (Receptors, Odorant) 0 (Insect Repellents) 0 (Insect Proteins) ML9LGA7468 (Phenylethyl Alcohol) |
| Entry Date(s): | Date Created: 20250901 Date Completed: 20250910 Latest Revision: 20250910 |
| Update Code: | 20250910 |
| DOI: | 10.1021/acs.jafc.5c07129 |
| PMID: | 40887884 |
| Databáza: | MEDLINE |
Nájsť tento článok vo Web of Science | Abstrakt: | The rice moth Corcyra cephalonica , a highly destructive pest found worldwide, with a wide-ranging diet and robust reproduction, severely damages various stored foods. A sensitive olfactory system is essential for insect behavioral perception, odorant receptors (ORs) play a crucial role in detecting volatile chemical cues in the living environment of insects. However, the reports on the identification and functional analyses of ORs in C. cephalonica are currently lacking. In this study, we identified CcepOR13, which clusters into the conserved lepidopteran OR42 ortholog family. TEVC recordings revealed its conserved function, and phenylacetaldehyde, 2-phenylethanol, cinnamaldehyde, and pentanal were identified as its main ligands. Behavioral experiments revealed that among these four ligands, C. cephalonica exhibited avoidance behavior only in response to 2-phenylethanol, and after dsRNA-CcepOR13 treatment, its electrophysiological and avoidance responses to 2-phenylethanol were significantly reduced. Our findings indicate that CcepOR13 is essential for 2-phenylethanol recognition by C. cephalonica , suggesting that the development of repellents for biological control is warranted. |
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| ISSN: | 1520-5118 |
| DOI: | 10.1021/acs.jafc.5c07129 |