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Direct real-time determination of photosynthetic efficiency by photo-calorespirometry using Synochocystis sp. PCC 6803 as model cyanobacterium.

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Titel: Direct real-time determination of photosynthetic efficiency by photo-calorespirometry using Synochocystis sp. PCC 6803 as model cyanobacterium.
Autoren: Linke T; Helmholtz Center for Environmental Research (UFZ) Leipzig, Permoserstraße 15, D-04318, Germany., Paufler S; Helmholtz Center for Environmental Research (UFZ) Leipzig, Permoserstraße 15, D-04318, Germany., Dusny C; Helmholtz Center for Environmental Research (UFZ) Leipzig, Permoserstraße 15, D-04318, Germany., Maskow T; Helmholtz Center for Environmental Research (UFZ) Leipzig, Permoserstraße 15, D-04318, Germany. Electronic address: Thomas.maskow@ufz.de., Schmid A; Helmholtz Center for Environmental Research (UFZ) Leipzig, Permoserstraße 15, D-04318, Germany.
Quelle: Bioresource technology [Bioresour Technol] 2026 Jan; Vol. 439, pp. 133226. Date of Electronic Publication: 2025 Aug 27.
Publikationsart: Journal Article
Sprache: English
Info zur Zeitschrift: Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s): Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
MeSH-Schlagworte: Photosynthesis*/physiology , Photosynthesis*/radiation effects , Synechocystis*/physiology , Synechocystis*/metabolism , Calorimetry*/methods , Models, Biological* , Cyanobacteria*/physiology , Cyanobacteria*/metabolism, Light ; Oxygen/metabolism ; Thermodynamics
Abstract: Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Photosynthetic efficiency (PE) is key to evaluating phototrophic organisms in biotechnological applications. However, current methods offer limited, indirect insights with poor time resolution. To address this, photo-calorespirometry (Photo-CR) was developed, a novel, non-invasive technique for real-time, direct quantification of photosynthetic energy conversion. Photo-CR simultaneously measures metabolic heat flow and oxygen evolution, capturing both light and dark reactions, including all heat-dissipating side-reactions. Using Synechocystis sp. PCC 6803, it revealed a maximum conserved energy flux of 5.2 ± 0.8 Wg CDW-1 and a light energy yield of 26.4 ± 5.8 kJg CDW-1 . A strong correlation between heat and oxygen flux yielded an oxycaloric equivalent of 488.5 ± 0.3 kJmol O2-1 . PE dynamics, including light saturation and adaptation to fluctuating light, were characterized, showing a maximum PE of 2.3 ± 0.4 % and half-saturation at 360 μmol Photons m -2 s -1 . Photo-CR integrates thermodynamic and respiratory data, offering novel insights into side-reactions like the Mehler cycle and enabling advanced screening and optimization in photo-biotechnology.
(Copyright © 2025 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Contributed Indexing: Keywords: Calorimetry; Oxycaloric equivalent; Photosynthesis; Photosynthetic adaptation mechanisms; Phototrophic microorganisms; Renewable energy
Substance Nomenclature: S88TT14065 (Oxygen)
Entry Date(s): Date Created: 20250829 Date Completed: 20251015 Latest Revision: 20251015
Update Code: 20251015
DOI: 10.1016/j.biortech.2025.133226
PMID: 40882928
Datenbank: MEDLINE
Beschreibung
Abstract:Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br />Photosynthetic efficiency (PE) is key to evaluating phototrophic organisms in biotechnological applications. However, current methods offer limited, indirect insights with poor time resolution. To address this, photo-calorespirometry (Photo-CR) was developed, a novel, non-invasive technique for real-time, direct quantification of photosynthetic energy conversion. Photo-CR simultaneously measures metabolic heat flow and oxygen evolution, capturing both light and dark reactions, including all heat-dissipating side-reactions. Using Synechocystis sp. PCC 6803, it revealed a maximum conserved energy flux of 5.2 ± 0.8 Wg <subscript>CDW</subscript><sup>-1</sup> and a light energy yield of 26.4 ± 5.8 kJg <subscript>CDW</subscript><sup>-1</sup> . A strong correlation between heat and oxygen flux yielded an oxycaloric equivalent of 488.5 ± 0.3 kJmol <subscript>O2</subscript><sup>-1</sup> . PE dynamics, including light saturation and adaptation to fluctuating light, were characterized, showing a maximum PE of 2.3 ± 0.4 % and half-saturation at 360 μmol <subscript>Photons</subscript> m <sup>-2</sup> s <sup>-1</sup> . Photo-CR integrates thermodynamic and respiratory data, offering novel insights into side-reactions like the Mehler cycle and enabling advanced screening and optimization in photo-biotechnology.<br /> (Copyright © 2025 The Authors. Published by Elsevier Ltd.. All rights reserved.)
ISSN:1873-2976
DOI:10.1016/j.biortech.2025.133226