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Assessment of a Low-Cost Mosquito-Borne Disease Early Warning System Integrating Molecular Mosquito and Arbovirus Surveillance.

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Title: Assessment of a Low-Cost Mosquito-Borne Disease Early Warning System Integrating Molecular Mosquito and Arbovirus Surveillance.
Authors: Jurado-Sánchez N; Viral & Human Genomics BSL-3 Laboratory, Faculty of Medicine, UASLP, San Luis Potosí, Mexico., Espinosa-Reyes G; Coordination for the Innovation and Application of Science and Technology (CIACYT), WHO Collaborating Centre on Health Risk Assessment and Children's Environmental Health, Faculty of Medicine, UASLP, San Luis Potosí, Mexico., Comas-García A; Department of Microbiology, Faculty of Medicine, UASLP, San Luis Potosí, Mexico.; Faculty of Medicine, Universidad Cuauhtémoc, San Luis Potosí, San Luis Potosí, Mexico., Barriga-Martínez FD; Coordination for the Innovation and Application of Science and Technology (CIACYT), WHO Collaborating Centre on Health Risk Assessment and Children's Environmental Health, Faculty of Medicine, UASLP, San Luis Potosí, Mexico., Comas-García M; Health and Biomedical Sciences Research Centre (CICSaB), UASLP, San Luis Potosí, Mexico.; Faculty of Sciences, UASLP, San Luis Potosí, Mexico., Guerra-Palomares SE; Viral & Human Genomics BSL-3 Laboratory, Faculty of Medicine, UASLP, San Luis Potosí, Mexico., García-Sepúlveda CA; Viral & Human Genomics BSL-3 Laboratory, Faculty of Medicine, UASLP, San Luis Potosí, Mexico.
Source: Zoonoses and public health [Zoonoses Public Health] 2025 Nov; Vol. 72 (7), pp. 669-682. Date of Electronic Publication: 2025 Aug 04.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Blackwell Verlag Country of Publication: Germany NLM ID: 101300786 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1863-2378 (Electronic) Linking ISSN: 18631959 NLM ISO Abbreviation: Zoonoses Public Health Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin, Germany : Blackwell Verlag
MeSH Terms: Arboviruses*/isolation & purification , Mosquito Vectors*/virology , Culicidae*/virology , Arbovirus Infections*/epidemiology , Arbovirus Infections*/virology, Animals ; Mexico/epidemiology ; Humans ; Mosquito-Borne Diseases
Abstract: Introduction: Arboviruses can be transmitted by Anophelinae (Anopheles genus) or Culicinae (Aedes and Culex genera) mosquitoes. Ecological and sociodemographic factors such as urbanisation, poverty, access to health systems and social inequality determine vector density and risk of disease transmission. Effective surveillance of vectors and arboviruses is crucial for guiding public health strategies.
Methods: We developed a low-cost molecular approach integrating mosquito and arbovirus surveillance and assessed its performance during 2021 in San Luis Potosí, Mexico. Our approach incorporates an innovative mosquito trap (Yoy trap), an ITS2-based mosquito molecular taxonomy assay and the use of FTA card preservation of arboviral RNA.
Results: A total of 16,319 mosquitoes were collected, Culex spp. genus being the most abundant (63.3%) followed by Aedes spp. (26.6%) and Anopheles spp. (4.7%). Our approach allowed us to characterise mosquito population dynamics including the centripetal expansion of mosquito range from city outskirts to urban areas. Viral RNA screening of 124 FTA cards identified the presence of four arboviruses in mosquitoes of the city of San Luis Potosi: DENV (6.5%), ZIKV (5.6%), CHIKV (1.6%) and WNV (3.2%).
Conclusions: This surveillance system detected DENV in mosquitoes 7 weeks prior to the first reported human case, further supporting the public health benefits deriving from the adoption of similar innovative, low-cost and robust surveillance systems.
(© 2025 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
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Grant Information: CBF-2025-I-3953 Secretariat of Science, Humanities, Technology and Innovation (SECIHT):
Contributed Indexing: Keywords: Aedes; Anopheles; Culex; Zika; chikungunya; dengue
Entry Date(s): Date Created: 20250805 Date Completed: 20251009 Latest Revision: 20251009
Update Code: 20251009
DOI: 10.1111/zph.70005
PMID: 40760766
Database: MEDLINE
Description
Abstract:Introduction: Arboviruses can be transmitted by Anophelinae (Anopheles genus) or Culicinae (Aedes and Culex genera) mosquitoes. Ecological and sociodemographic factors such as urbanisation, poverty, access to health systems and social inequality determine vector density and risk of disease transmission. Effective surveillance of vectors and arboviruses is crucial for guiding public health strategies.<br />Methods: We developed a low-cost molecular approach integrating mosquito and arbovirus surveillance and assessed its performance during 2021 in San Luis Potosí, Mexico. Our approach incorporates an innovative mosquito trap (Yoy trap), an ITS2-based mosquito molecular taxonomy assay and the use of FTA card preservation of arboviral RNA.<br />Results: A total of 16,319 mosquitoes were collected, Culex spp. genus being the most abundant (63.3%) followed by Aedes spp. (26.6%) and Anopheles spp. (4.7%). Our approach allowed us to characterise mosquito population dynamics including the centripetal expansion of mosquito range from city outskirts to urban areas. Viral RNA screening of 124 FTA cards identified the presence of four arboviruses in mosquitoes of the city of San Luis Potosi: DENV (6.5%), ZIKV (5.6%), CHIKV (1.6%) and WNV (3.2%).<br />Conclusions: This surveillance system detected DENV in mosquitoes 7 weeks prior to the first reported human case, further supporting the public health benefits deriving from the adoption of similar innovative, low-cost and robust surveillance systems.<br /> (© 2025 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
ISSN:1863-2378
DOI:10.1111/zph.70005