Immune function and transcriptome of hemocytes in northern quahogs Mercenaria mercenaria in response to acute salinity challenges.
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| Názov: | Immune function and transcriptome of hemocytes in northern quahogs Mercenaria mercenaria in response to acute salinity challenges. |
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| Autori: | Zeng Y; School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, 7922 NW 71st Street, Gainesville, FL, 32653, USA., Zeng X; School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, 7922 NW 71st Street, Gainesville, FL, 32653, USA., Yee JC; School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, 7922 NW 71st Street, Gainesville, FL, 32653, USA., Yang H; School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, 7922 NW 71st Street, Gainesville, FL, 32653, USA. Electronic address: huipingyang@ufl.edu. |
| Zdroj: | Fish & shellfish immunology [Fish Shellfish Immunol] 2025 Nov; Vol. 166, pp. 110609. Date of Electronic Publication: 2025 Jul 31. |
| Spôsob vydávania: | Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London ; New York : Academic Press, c1991- |
| Výrazy zo slovníka MeSH: | Hemocytes*/immunology , Salinity* , Transcriptome*/immunology , Mercenaria*/immunology , Mercenaria*/genetics , Immunity, Innate*/genetics, Animals |
| Abstrakt: | Competing Interests: Declaration of competing interests All authors declare no conflict of interest. The goal of this study was to evaluate effects of short-term acute salinity challenges on the hemocyte physiology in northern quahogs, Mercenaria mercenaria, an important aquaculture species in the U.S. The objectives were to: 1) challenge adult northern quahogs with salinities of 5, 15, 25 (control), 35, and 45 ppt; 2) evaluate cellular responses, including hemocyte concentration, viability, phagocytosis rate, ROS production, and lysosomal presence at 12 h, 24 h, 48 h, 72 h, 144 h, and 21 days post-challenge; and 3) evaluate the molecular response of hemocytes after 72 h challenge. M. mercenaria at 5, 15, 25, 35 ppt exposure showed no mortality, while at 45 ppt showed a 100 % mortality at day 7, reflecting better tolerance to hyposalinity. Changes of hemolymph osmolality at different salinities and exposure periods indicated M. mercenaria equilibrates with the environmental seawater within its tolerance range (15-35 ppt) as an osmoconformer. Hemocyte concentration increased significantly at 5, 15, and 45 ppt stress. Hemocyte immune functions exhibited a varied pattern in response to different salinity levels, enabling them to resolve stresses. The RNAseq of hemocytes revealed DEGs in response to salinity challenges, specifically enrichment of amino acid transporters (SLC2A5, SLC6A1, SLC5A8, SLC1A3, SLC25A38), immune response (CHAC2, MGST3, IRF1, and IRFD2) and cell signaling (Wnt, TRAIL-activated and Toll) pathway in hyper/hypo salinity groups, indicated their importance in salinity stress responses. This study provided critical insights into the cellular and molecular responses of M. mercenaria hemocytes to salinity challenges. (Copyright © 2025 Elsevier Ltd. All rights reserved.) |
| Contributed Indexing: | Keywords: Flow cytometry; Hemocyte; Immune assay; Mercenaria mercenaria; Salinity challenge; Transcriptome |
| Entry Date(s): | Date Created: 20250802 Date Completed: 20250915 Latest Revision: 20250915 |
| Update Code: | 20250916 |
| DOI: | 10.1016/j.fsi.2025.110609 |
| PMID: | 40752840 |
| Databáza: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Competing Interests: Declaration of competing interests All authors declare no conflict of interest.<br />The goal of this study was to evaluate effects of short-term acute salinity challenges on the hemocyte physiology in northern quahogs, Mercenaria mercenaria, an important aquaculture species in the U.S. The objectives were to: 1) challenge adult northern quahogs with salinities of 5, 15, 25 (control), 35, and 45 ppt; 2) evaluate cellular responses, including hemocyte concentration, viability, phagocytosis rate, ROS production, and lysosomal presence at 12 h, 24 h, 48 h, 72 h, 144 h, and 21 days post-challenge; and 3) evaluate the molecular response of hemocytes after 72 h challenge. M. mercenaria at 5, 15, 25, 35 ppt exposure showed no mortality, while at 45 ppt showed a 100 % mortality at day 7, reflecting better tolerance to hyposalinity. Changes of hemolymph osmolality at different salinities and exposure periods indicated M. mercenaria equilibrates with the environmental seawater within its tolerance range (15-35 ppt) as an osmoconformer. Hemocyte concentration increased significantly at 5, 15, and 45 ppt stress. Hemocyte immune functions exhibited a varied pattern in response to different salinity levels, enabling them to resolve stresses. The RNAseq of hemocytes revealed DEGs in response to salinity challenges, specifically enrichment of amino acid transporters (SLC2A5, SLC6A1, SLC5A8, SLC1A3, SLC25A38), immune response (CHAC2, MGST3, IRF1, and IRFD2) and cell signaling (Wnt, TRAIL-activated and Toll) pathway in hyper/hypo salinity groups, indicated their importance in salinity stress responses. This study provided critical insights into the cellular and molecular responses of M. mercenaria hemocytes to salinity challenges.<br /> (Copyright © 2025 Elsevier Ltd. All rights reserved.) |
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| ISSN: | 1095-9947 |
| DOI: | 10.1016/j.fsi.2025.110609 |