Synthesis of Arbitrary Interference Patterns Using a Single Galvanometric Mirror and Its Application to Structured Illumination Microscopy.
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| Titel: | Synthesis of Arbitrary Interference Patterns Using a Single Galvanometric Mirror and Its Application to Structured Illumination Microscopy. |
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| Autoren: | Guo K; Department of Bioengineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K., Boualam A; Department of Bioengineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K., Manton JD; MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, U.K., Rowlands CJ; Department of Bioengineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K. |
| Quelle: | ACS photonics [ACS Photonics] 2025 Jun 19; Vol. 12 (7), pp. 3635-3643. Date of Electronic Publication: 2025 Jun 19 (Print Publication: 2025). |
| Publikationsart: | Journal Article |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101634366 Publication Model: eCollection Cited Medium: Print ISSN: 2330-4022 (Print) Linking ISSN: 23304022 NLM ISO Abbreviation: ACS Photonics Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : American Chemical Society, [2014-] |
| Abstract: | Structured illumination microscopy (SIM) overcomes the diffraction limit of optical microscopy by projecting finely spaced interference fringes with different orientations and phases onto the sample and imaging the result. A major challenge of SIM is to generate the different illumination patterns with a high contrast and switching speed, which commonly requires expensive devices and the sacrifice of illumination power efficiency. We present a new way of generating interference patterns for 2D and 3D SIM achromatically, with high speed and high power efficiency, using only one moving part. The interference patterns are created by a common-path interferometer, with the orientation, polarization, and phase of interference patterns controlled by a single galvanometric mirror. We characterize the contrast and switching speed of the interference patterns and demonstrate their utility by performing high-speed (980 raw frames per second) 2D SIM imaging on fluorescent nanoparticles and 3D SIM on fixed iFluor 488 phalloidin-stained U-2 OS cells. (© 2025 The Authors. Published by American Chemical Society.) |
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| Contributed Indexing: | Keywords: fringe projection; galvanometer; high-throughput imaging; interference; microscopy; structured illumination microscopy; super-resolution |
| Entry Date(s): | Date Created: 20250721 Latest Revision: 20250723 |
| Update Code: | 20250723 |
| PubMed Central ID: | PMC12272674 |
| DOI: | 10.1021/acsphotonics.5c00516 |
| PMID: | 40688185 |
| Datenbank: | MEDLINE |
Nájsť tento článok vo Web of Science | Abstract: | Structured illumination microscopy (SIM) overcomes the diffraction limit of optical microscopy by projecting finely spaced interference fringes with different orientations and phases onto the sample and imaging the result. A major challenge of SIM is to generate the different illumination patterns with a high contrast and switching speed, which commonly requires expensive devices and the sacrifice of illumination power efficiency. We present a new way of generating interference patterns for 2D and 3D SIM achromatically, with high speed and high power efficiency, using only one moving part. The interference patterns are created by a common-path interferometer, with the orientation, polarization, and phase of interference patterns controlled by a single galvanometric mirror. We characterize the contrast and switching speed of the interference patterns and demonstrate their utility by performing high-speed (980 raw frames per second) 2D SIM imaging on fluorescent nanoparticles and 3D SIM on fixed iFluor 488 phalloidin-stained U-2 OS cells.<br /> (© 2025 The Authors. Published by American Chemical Society.) |
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| ISSN: | 2330-4022 |
| DOI: | 10.1021/acsphotonics.5c00516 |