Exogenous M2 Macrophage-Loaded Scaffolds Promote Early Periodontal Regeneration by Improving Osteogenic Differentiation, Cell Homing, and Angiogenesis.
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| Titel: | Exogenous M2 Macrophage-Loaded Scaffolds Promote Early Periodontal Regeneration by Improving Osteogenic Differentiation, Cell Homing, and Angiogenesis. |
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| Autoren: | Liao Z; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Liang L; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Fang H; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Cui Y; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Liang C; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Huang G; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Dai Z; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Song Z; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Yang H; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China., Huang J; Yunnan Key Laboratory of Stomatology, School of Stomatology, Kunming Medical University, Kunming, Yunnan, 650500, China. |
| Quelle: | Advanced healthcare materials [Adv Healthc Mater] 2025 Sep; Vol. 14 (25), pp. e2501428. Date of Electronic Publication: 2025 Jul 11. |
| Publikationsart: | Journal Article |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101581613 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2192-2659 (Electronic) Linking ISSN: 21922640 NLM ISO Abbreviation: Adv Healthc Mater Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Weinheim : Wiley-VCH, 2012- |
| MeSH-Schlagworte: | Osteogenesis*/drug effects , Tissue Scaffolds*/chemistry , Macrophages*/metabolism , Macrophages*/cytology , Cell Differentiation*/drug effects , Neovascularization, Physiologic*/drug effects , Regeneration* , Periodontium*/physiology, Animals ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Mice ; Male ; Angiogenesis |
| Abstract: | Manipulating the immune microenvironment (including the macrophage phenotype) by tissue implants is an important direction in the field of tissue regeneration. This study reports a macrophage-loaded hybrid scaffold construction to improve the response efficiency of implant-macrophage-tissue regeneration axis and precisely polarize macrophages toward the pro-regenerative M2 phenotype in vitro, and constructed an M2 macrophage-loaded hybrid scaffold. The feasibility of using a macrophage-loaded hybrid scaffold for immune-mediated periodontal regeneration is studied in vivo and in vitro. The results illustrate that the M2 macrophage-loaded hybrid scaffolds promoted the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in vitro and promoted cell recruitment and angiogenesis in vivo to perform well in early in vivo osteogenesis. The macrophage-loaded hybrid scaffold proposed in this study plays an important role in promoting periodontal regeneration, which enriches the theory and strategy of macrophage phenotype regulation-mediated periodontal regeneration, and provides inspiration and reference for using macrophage-loaded hybrid scaffolds in regenerating other tissues. (© 2025 Wiley‐VCH GmbH.) |
| References: | T. E. Van Dyke, J Periodontol 2008, 79, 1601. H. N. Woo, Y. J. Cho, S. Tarafder, C. H. Lee, Bioact Mater 2021, 6, 3328. Y. Cui, S. Hong, Y. Xia, X. Li, X. He, X. Hu, Y. Li, X. Wang, K. Lin, L. Mao, Adv. Sci. (Weinh) 2023, 10, 202302029. K. X. Tian Xu, C. Wang, S. Ivanovski, Y. Zhou, Nanoscale 2023, 15, 5992. X. T. He, X. Li, M. Zhang, B. M. Tian, L. J. Sun, C. S. Bi, D. K. Deng, H. Zhou, H. L. Qu, C. Wu, F. M. Chen, Biomaterials 2022, 283, 121439. E. Sadeghian Dehkord, B. De Carvalho, M. Ernst, A. Albert, F. Lambert, L. Geris, Mater Today Bio 2024, 26, 101100. A. Sculean, D. Nikolidakis, G. Nikou, A. Ivanovic, I. L. C. Chapple, A. Stavropoulos, Periodontol 2000 2015, 68, 182. K. L. Spiller, T. J. Koh, Adv Drug Deliv Rev 2017, 122, 74. K. Liddiard, P. R. Taylor, Nat. Med. 2015, 21, 119. M. C. Walsh, N. Takegahara, H. Kim, Y. Choi, Nat Rev Rheumatol 2018, 14, 146. J. Lee, H. Byun, S. K. Madhurakkat Perikamana, S. Lee, H. Shin, Adv. Healthcare Mater. 2018, 8, 1801106. P. J. Murray, J. E. Allen, S. K. Biswas, E. A. Fisher, D. W. Gilroy, S. Goerdt, S. Gordon, J. A. Hamilton, L. B. Ivashkiv, T. Lawrence, M. Locati, A. Mantovani, F. O. Martinez, J. L. Mege, D. M. Mosser, G. Natoli, J. P. Saeij, J. L. Schultze, K. A. Shirey, A. Sica, J. Suttles, I. Udalova, J. A. van Ginderachter, S. N. Vogel, T. A. Wynn, Immunity 2014, 41, 14. J. Xue, S. V. Schmidt, J. Sander, A. Draffehn, W. Krebs, I. Quester, D. De Nardo, T. D. Gohel, M. Emde, L. Schmidleithner, H. Ganesan, A. Nino‐Castro, M. R. Mallmann, L. Labzin, H. Theis, M. Kraut, M. Beyer, E. Latz, T. C. Freeman, T. Ulas, J. L. Schultze, Immunity 2014, 40, 274. N. Wang, H. Liang, K. Zen, Front Immunol 2014, 6, 614. C. Aguzzi, O. Marinelli, L. Zeppa, G. Santoni, F. Maggi, M. Nabissi, Biomed. Pharmacother. 2020, 132, 110845. Á. Arnosa‐Prieto, P. Diaz‐Rodriguez, M. A. González‐Gómez, P. García‐Acevedo, L. de Castro‐Alves, Y. Piñeiro, J. Rivas, J. Colloid Interface Sci. 2024, 655, 286. K. Ishida, T. Nagatake, A. Saika, S. Kawai, E. Node, K. Hosomi, J. Kunisawa, Front Immunol 2023, 14, 1111729. X. Liu, B. Gaihre, S. Park, L. Li, B. Dashtdar, M. D. Astudillo Potes, A. Terzic, B. D. Elder, L. Lu, Bioact Mater 2023, 27, 216. L. Junxian, M. Mehrabanian, H. Mivehchi, M. Banakar, E. A. Etajuri, Oral Dis 2023, 29, 2552. X. T. He, X. Li, Y. Xia, Y. Yin, R. X. Wu, H. H. Sun, F. M. Chen, Corrigendum to ‘Building capacity for macrophage modulation and stem cell recruitment in high‐stiffness hydrogels for complex periodontal regeneration: Experimental studies in vitro and in rats’ [Acta Biomaterialia 88 2019 162‐180], Acta Biomater 150 (2022) 456. Y. He, M. Tian, X. Li, J. Hou, S. Chen, G. Yang, X. Liu, S. Zhou, Adv. Healthcare Mater. 2022, 11, 2102236. M. Wu, H. Liu, D. Li, Y. Zhu, P. Wu, Z. Chen, F. Chen, Y. Chen, Z. Deng, L. Cai, Adv. Sci. (Weinh) 2024, 11, 2304641. M. Wu, H. Liu, Y. Zhu, F. Chen, Z. Chen, L. Guo, P. Wu, G. Li, C. Zhang, R. Wei, L. Cai, Small 2023, 19, 2300111. M. Wu, H. Liu, Y. Zhu, P. Wu, Y. Chen, Z. Deng, X. Zhu, L. Cai, Theranostics 2024, 14, 4014. Y. Zhu, H. Liu, P. Wu, Y. Chen, Z. Deng, L. Cai, M. Wu, Theranostics 2024, 14, 7140. Y. Li, F. Muhammad, X. Chen, D. Gu, W. Li, J. Tang, M. Cheng, J. Du, S. Qiao, Y. Deng, Q. Yu, H. Wei, L. Miao, Nano Today 2025, 61, 102624. L. Ouyang, B. Chen, X. Liu, D. Wang, Y. Li, Y. Liao, K. W. K. Yeung, X. Liu, Bioact Mater 2022, 21, 520. N. Yan, J. Xu, G. Liu, C. Ma, L. Bao, Y. Cong, Z. Wang, Y. Zhao, W. Xu, C. Chen, ACS Nano 2022, 16, 18253. T. Ding, W. Kang, J. Li, L. Yu, S. Ge, J. Nanobiotechnol. 2021, 19, 247. N. Su, C. Villicana, D. Barati, P. Freeman, Y. Luo, F. Yang, Adv. Mater. 2023, 35, 2208781. C. Wang, Y. Chiu, A. K. Lee, Y. A. Lin, P. Y. Lin, M. Shie, Biomedicines 2021, 9, 431. Y. Wang, C. Li, Y. Wan, M. Qi, Q. Chen, Y. Sun, X. Sun, J. Fang, L. Fu, L. Xu, B. Dong, L. Wang, Small 2021, 17, 2101505. S. Wu, J. Ma, J. Liu, C. Liu, S. Ni, T. Dai, Y. Wang, Y. Weng, H. Zhao, D. Zhou, X. Zhao, ACS Appl. Mater. Interfaces 2022, 14, 15942. S. M. Hull, L. G. Brunel, S. C. Heilshorn, Adv. Mater. 2022, 34, 2103691. Y. Niu, Z. Wang, Y. Shi, L. Dong, C. Wang, Bioact Mater 2020, 6, 244. A. Ho‐Shui‐Ling, J. Bolander, L. E. Rustom, A. W. Johnson, F. P. Luyten, C. Picart, Biomaterials 2018, 180, 143. Z. Liao, L. Liang, L. Ma, L. Wang, Z. Song, J. Huang, H. Yang, Mater. Lett. 2024, 371, 136908. S. Soni, Biotechnol. Appl. Biochem. 2022, 69, 265. B. Gao, R. Deng, Y. Chai, H. Chen, B. Hu, X. Wang, S. Zhu, Y. Cao, S. Ni, M. Wan, L. Yang, Z. Luo, X. Cao, J. Clin. Invest. 2019, 129, 2578. S. Gao, L. Wang, Y. Zhang, L. Li, Y. Zhang, X. Gao, J. Mao, L. Wang, L. Wang, H. Wang, M. Zhu, G. Fan, Appl. Mater. Today 2020, 21, 100841. Y. Wang, W. Chen, S. Qiao, H. Zou, X. J. Yu, Y. Yang, Z. Li, J. Wang, M. S. Chen, J. Xu, L. Zheng, Cell Mol. Immunol. 2024, 21, 1120. X. Pei, L. Liu, J. Wang, C. Guo, Q. Li, J. Li, Q. Ren, R. Ma, Y. Zheng, Y. Zhang, L. Liu, D. Zheng, P. Wang, P. Jiang, X. Feng, E. Jiang, Y. Wang, S. Feng, Nat. Commun. 2024, 15, 691. H. Liu, H. Zhou, Y. Fan, J. Li, Z. Guo, Q. Xu, Y. Liu, K. Gao, N. A. Lahcine, J. Zhang, J. Zhou, F. Guo, C. Liu, J. Bone Miner. Res. 2024, zjae198. C. Yang, C. Zhao, X. Wang, M. Shi, Y. Zhu, L. Jing, C. Wu, J. Chang, Nanoscale 2019, 11, 17699. J. Liu, F. Chen, L. Tian, J. Wu, K. Liu, Q. Wan, B. Yuan, X. Zhu, X. Chen, X. Zhang, Collagen Leather 2024, 6, 13. T. A. Wynn, K. M. Vannella, Immunity 2016, 44, 450. Y. Ha, X. Ma, S. Li, T. Li, Z. Li, Y. Qian, M. Shafiq, J. Wang, X. Zhou, C. He, Adv. Funct. Mater. 2022, 32, 202200011. B. Bai, Y. Liu, J. Huang, S. Wang, H. Chen, Y. Huo, H. Zhou, Y. Liu, S. Feng, G. Zhou, Y. Hua, Adv. Sci. (Weinh) 2024, 11, 2305580. H. Byun, Y. Han, E. Kim, I. Jun, J. Lee, H. Jeong, S. J. Huh, J. Joo, S. R. Shin, H. Shin, Bioact Mater 2024, 36, 185. Y. Xiong, B. B. Mi, M. A. Shahbazi, T. Xia, J. Xiao, Mil Med Res 2024, 11, 69. Y. Hou, W. Xie, L. Yu, L. C. Camacho, C. Nie, M. Zhang, R. Haag, Q. Wei, Small 2020, 16, 1905422. Y. Feng, D. Wu, J. Knaus, S. Keßler, B. Ni, Z. Chen, J. Avaro, R. Xiong, H. Cölfen, Z. Wang, Adv. Healthcare Mater. 2023, 12, 2203411. A. M. C. Barradas, H. Yuan, J. van der Stok, B. Le Quang, H. Fernandes, A. Chaterjea, M. C. H. Hogenes, K. Shultz, L. R Donahue, C. van Blitterswijk, J. de Boer, Biomaterials 2012, 33, 5696. X. Shen, X. Shen, B. Li, W. Zhu, Y. Fu, R. Xu, Y. Du, J. Cheng, H. Jiang, Bone 2021, 143, 115618. K. Sadtler, K. Estrellas, B. W. Allen, M. T. Wolf, H. Fan, A. J. Tam, C. H. Patel, B. S. Luber, H. Wang, K. R. Wagner, J. D. Powell, F. Housseau, D. M. Pardoll, J. H. Elisseeff, Science 2016, 352, 366. Z. Liu, H. Liu, S. Liu, B. Li, Y. Liu, E. Luo, Cell Prolif 2024, 57, 13596. M. W. Laschke, M. D. Menger, Trends Biotechnol. 2022, 40, 281. H. M. Kronenberg, J Dent Res 2016, 95, 1221. C. J. Percival, J. T. Richtsmeier, Dev. Dyn. 2013, 242, 909. G. Zhu, T. Zhang, M. Chen, K. Yao, X. Huang, B. Zhang, Y. Li, J. Liu, Y. Wang, Z. Zhao, Bioact Mater 2021, 6, 4110. T. Yamada, H. Kawano, Y. Koshizuka, T. Fukuda, K. Yoshimura, S. Kamekura, T. Saito, T. Ikeda, Y. Kawasaki, Y. Azuma, S. Ikegawa, K. Hoshi, U. I. Chung, K. Nakamura, S. Kato, H. Kawaguchi, Nat. Med. 2006, 12, 665. |
| Grant Information: | 82201015 National Natural Science Foundation of China; 82260186 National Natural Science Foundation of China; 2024XKTDTS09 First-Class Discipline Team of Kunming Medical University; 202301AY070001-038 Applied Basic Research Foundation of Yunnan Province; 2022GZ002 Scientific Research Fund of Clinical Medical Research Center of Oral Diseases of Yunnan Province |
| Contributed Indexing: | Keywords: angiogenesis; macrophage; periodontal regeneration; scaffold |
| Entry Date(s): | Date Created: 20250711 Date Completed: 20250929 Latest Revision: 20250929 |
| Update Code: | 20250929 |
| DOI: | 10.1002/adhm.202501428 |
| PMID: | 40643064 |
| Datenbank: | MEDLINE |
Nájsť tento článok vo Web of Science | Abstract: | Manipulating the immune microenvironment (including the macrophage phenotype) by tissue implants is an important direction in the field of tissue regeneration. This study reports a macrophage-loaded hybrid scaffold construction to improve the response efficiency of implant-macrophage-tissue regeneration axis and precisely polarize macrophages toward the pro-regenerative M2 phenotype in vitro, and constructed an M2 macrophage-loaded hybrid scaffold. The feasibility of using a macrophage-loaded hybrid scaffold for immune-mediated periodontal regeneration is studied in vivo and in vitro. The results illustrate that the M2 macrophage-loaded hybrid scaffolds promoted the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in vitro and promoted cell recruitment and angiogenesis in vivo to perform well in early in vivo osteogenesis. The macrophage-loaded hybrid scaffold proposed in this study plays an important role in promoting periodontal regeneration, which enriches the theory and strategy of macrophage phenotype regulation-mediated periodontal regeneration, and provides inspiration and reference for using macrophage-loaded hybrid scaffolds in regenerating other tissues.<br /> (© 2025 Wiley‐VCH GmbH.) |
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| ISSN: | 2192-2659 |
| DOI: | 10.1002/adhm.202501428 |