Unravelling Immunogenic Cell Death Mechanisms by Functional Proteomics Arrays.
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| Title: | Unravelling Immunogenic Cell Death Mechanisms by Functional Proteomics Arrays. |
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| Authors: | Nuño-Soriano A; Department of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), IBSAL, Universidad de Salamanca-CSIC, Salamanca, Spain.; Proteomics Unit, Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain., Arias-Hidalgo C; Department of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), IBSAL, Universidad de Salamanca-CSIC, Salamanca, Spain.; Proteomics Unit, Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain., Juanes-Velasco P; Department of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), IBSAL, Universidad de Salamanca-CSIC, Salamanca, Spain.; Proteomics Unit, Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain., Gongora R; Department of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), IBSAL, Universidad de Salamanca-CSIC, Salamanca, Spain., Hernández AP; Department of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), IBSAL, Universidad de Salamanca-CSIC, Salamanca, Spain. angytahg@usal.es.; Department of Pharmaceutical Sciences, Organic Chemistry, Faculty of Pharmacy, University of Salamanca, CIETUS, IBSAL, Salamanca, Spain. angytahg@usal.es., Fuentes M; Department of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), IBSAL, Universidad de Salamanca-CSIC, Salamanca, Spain. mfuentes@usal.es.; Proteomics Unit, Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain. mfuentes@usal.es. |
| Source: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2929, pp. 215-226. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| MeSH Terms: | Protein Array Analysis*/methods , Proteomics*/methods , Immunogenic Cell Death*, Humans ; Endoplasmic Reticulum Stress ; Signal Transduction |
| Abstract: | Immunogenic Cell Death (ICD) is a type of cell death regulated by an activation of endoplasmic reticulum stress. This signal results in an activation of the adaptative immune response against the affected cell. This characteristic has been used for the design of novel oncological drugs to complement antitumor therapy with this immunogenic response. However, ICD is complex and still needs a deep and massive study to unravel the key to its mechanism. Protein microarrays are a useful tool for large-scale protein detection and analysis, and thus may be the suitable platform for this study. In this study, antibody arrays have been designed and constructed as a method to detect different proteins involved in programmed cell death pathways in order to evaluate differential intracellular signalling profiles and correlate them with ICD. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Hernández ÁP, Juanes-Velasco P, Landeira-Viñuela A, Bareke H, Montalvillo E, Góngora R et al (2021) Restoring the immunity in the tumor microenvironment: insights into immunogenic cell death in onco-therapies. Cancers 13. MDPI AG. Liu Z et al (2023) Immunogenic cell death in hematological malignancy therapy. Adv Sci (Weinh) 10(13). Galluzzi L, Vitale I, Warren S, Adjemian S, Agostinis P, Martinez AB et al (2020) Consensus guidelines for the definition, detection and interpretation of immunogenic cell death. JITC 8. Galluzzi L, Buqué A, Kepp O, Zitvogel L, Kroemer G (2017) Immunogenic cell death in cancer and infectious disease. Nat Rev Immunol 17(2):97–111. (PMID: 10.1038/nri.2016.10727748397) Sprooten J, Laureano RS, Vanmeerbeek I, Govaerts J, Naulaerts S, Borras DM et al (2023) Trial watch: chemotherapy-induced immunogenic cell death in oncology. Onco Targets Ther 12(1). Juanes-Velasco P, Arias-Hidalgo C, Landeira-Viñuela A, Nuño-Soriano A, Fuentes-Vacas M, Góngora R et al (2023) Functional proteomics based on protein microarray technology for biomedical research. Adv Protein Chem Struct Biol. Bhawal R, Oberg AL, Zhang S, Kohli M (2020) Challenges and opportunities in clinical applications of blood-based proteomics in cancer. Cancers 12(9):2428. (PMID: 10.3390/cancers12092428328670437564506) Chen Z, Dodig-Crnković T, Schwenk JM, Tao SC (2018) Current applications of antibody microarrays. Clin Proteomics 15. Li S, Song G, Bai Y, Song N, Zhao J, Liu J et al (2021) Applications of protein microarrays in biomarker discovery for autoimmune diseases. Front Immunol 12. Matarraz S, González-González M, Jara M, Orfao A, Fuentes M (2011) New technologies in cancer. Protein microarrays for biomarker discovery. Clin Transl Oncol 13(3):156. (PMID: 10.1007/s12094-011-0635-821421460) Fernández-García J, García-Valiente R, Carabias-Sánchez J, Landeira-Viñuela A, Góngora R, Gonzalez-Gonzalez M, Fuentes M (2014) A systematic workflow for design and computational analysis of protein microarrays. In: Encyclopedia of biomedical engineering. Elsevier, pp 213–222. Juanes-Velasco P, Carabias-Sanchez J, Garcia-Valiente R, Fernandez-García J, Gongora R, Gonzalez-Gonzalez M, Fuentes M (2018) Microarrays as platform for multiplex assays in biomarker and drug discovery. In: Rapid test-advances in design, format and diagnostic applications. InTech, p 3. Landeira-Viñuela A, Juanes-Velasco P, Gongora R, Hernandez AP, Fuentes M (2021) Deciphering intracellular signaling pathways in tumoral pathologies. In: Protein microarrays for disease analysis: methods and protocols, pp 211–226. (PMID: 10.1007/978-1-0716-1562-1_15) Juanes-Velasco P, Landeira-Viñuela A, Hernandez A, Fuentes M (2021) Systematic and rational design of protein arrays in noncontact printers: pipeline and critical aspects. Methods Mol Biol:9–29. Hernández ÁP, Iglesias-Anciones L, Vaquero-González JJ, Piñol R, Criado JJ, Rodriguez E et al (2023) Enhancement of tumor cell immunogenicity and antitumor properties derived from platinum-conjugated iron nanoparticles. Cancers 15(12):3204. (PMID: 10.3390/cancers151232043737081310296818) Díez P, Dasilva N, González-González M, Matarraz S, Casado-Vela J, Orfao A et al (2012) Data analysis strategies for protein microarrays. Microarrays 1(2):64–83. (PMID: 10.3390/microarrays1020064276053365003438) |
| Contributed Indexing: | Keywords: Antibodies; Antibody microarrays; Cancer; Cell death; Functional proteomics |
| Entry Date(s): | Date Created: 20250702 Date Completed: 20250702 Latest Revision: 20250702 |
| Update Code: | 20250702 |
| DOI: | 10.1007/978-1-0716-4595-6_16 |
| PMID: | 40601154 |
| Database: | MEDLINE |
Nájsť tento článok vo Web of Science | Abstract: | Immunogenic Cell Death (ICD) is a type of cell death regulated by an activation of endoplasmic reticulum stress. This signal results in an activation of the adaptative immune response against the affected cell. This characteristic has been used for the design of novel oncological drugs to complement antitumor therapy with this immunogenic response. However, ICD is complex and still needs a deep and massive study to unravel the key to its mechanism. Protein microarrays are a useful tool for large-scale protein detection and analysis, and thus may be the suitable platform for this study. In this study, antibody arrays have been designed and constructed as a method to detect different proteins involved in programmed cell death pathways in order to evaluate differential intracellular signalling profiles and correlate them with ICD.<br /> (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| ISSN: | 1940-6029 |
| DOI: | 10.1007/978-1-0716-4595-6_16 |