Comparison of Different Solid-Phase Cleanup Methods Prior to the Detection of Ciguatoxins in Fish by Cell-Based Assay and LC-MS/MS.
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| Názov: | Comparison of Different Solid-Phase Cleanup Methods Prior to the Detection of Ciguatoxins in Fish by Cell-Based Assay and LC-MS/MS. |
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| Autori: | Sanchez-Henao A; IRTA, Marine and Continental Waters Program, Carretera de Poble Nou, 43540 La Ràpita, Catalonia, Spain.; IUSA, University Institute of Animal Health and Food Safety, University of Las Palmas de Gran Canaria, C/Trasmontaña S/N, Arucas, Canarias 35413, Spain., Real F; IUSA, University Institute of Animal Health and Food Safety, University of Las Palmas de Gran Canaria, C/Trasmontaña S/N, Arucas, Canarias 35413, Spain., Darias-Dágfeel Y; IUSA, University Institute of Animal Health and Food Safety, University of Las Palmas de Gran Canaria, C/Trasmontaña S/N, Arucas, Canarias 35413, Spain., García-Álvarez N; IUSA, University Institute of Animal Health and Food Safety, University of Las Palmas de Gran Canaria, C/Trasmontaña S/N, Arucas, Canarias 35413, Spain., Diogène J; IRTA, Marine and Continental Waters Program, Carretera de Poble Nou, 43540 La Ràpita, Catalonia, Spain., Rambla-Alegre M; IRTA, Marine and Continental Waters Program, Carretera de Poble Nou, 43540 La Ràpita, Catalonia, Spain. |
| Zdroj: | Journal of agricultural and food chemistry [J Agric Food Chem] 2025 Jun 11; Vol. 73 (23), pp. 14580-14591. Date of Electronic Publication: 2025 Jun 01. |
| Spôsob vydávania: | Journal Article; Comparative Study; Evaluation Study |
| Jazyk: | English |
| Informácie o časopise: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, American Chemical Society. |
| Výrazy zo slovníka MeSH: | Ciguatoxins*/isolation & purification , Ciguatoxins*/chemistry , Ciguatoxins*/analysis , Ciguatoxins*/metabolism , Tandem Mass Spectrometry*/methods , Fishes* , Food Contamination*/analysis , Solid Phase Extraction*/methods , Seafood*/analysis, Animals ; Chromatography, High Pressure Liquid/methods ; Chromatography, Liquid/methods ; Ciguatera Poisoning ; Liquid Chromatography-Mass Spectrometry |
| Abstrakt: | Ciguatera poisoning (CP) is the most reported food poisoning associated with fish consumption. Ciguatoxins (CTXs) are produced by microalgae and metabolized in fish; even low levels of these toxins in fish can lead to CP. To date, there is no unique validated methodology for their study, and demonstrating their presence in fish tissues is an analytical challenge. The main techniques used are cell-based assay and liquid chromatography, which may present different matrix effect interferences; thus, purification protocols are necessary. Six cleanup strategies for fish extracts, assessing the principal analogues found in fish in different parts of the world (CTX1B/CTX3C/C-CTX1), are compared here. Cleaned-up extracts are evaluated by cell-based assay and chromatography. All protocols are suitable for recovering the analogues of CTXs. Two of them, those that used polystyrene-divinylbenzene and silica cartridges, achieve the most adequate results showing toxicity in their fractions over 53% and chromatography efficiencies over 79% for CTX1B/CTX3C, proving to be the most versatile clean-ups for the study of the different CTX analogues. |
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| Contributed Indexing: | Keywords: LC-MS/MS; cell-based assay; ciguatoxins; cleanup; fish; solid phase extraction |
| Substance Nomenclature: | 11050-21-8 (Ciguatoxins) |
| Entry Date(s): | Date Created: 20250602 Date Completed: 20250611 Latest Revision: 20250615 |
| Update Code: | 20250617 |
| PubMed Central ID: | PMC12164330 |
| DOI: | 10.1021/acs.jafc.5c01142 |
| PMID: | 40452308 |
| Databáza: | MEDLINE |
Nájsť tento článok vo Web of Science | Abstrakt: | Ciguatera poisoning (CP) is the most reported food poisoning associated with fish consumption. Ciguatoxins (CTXs) are produced by microalgae and metabolized in fish; even low levels of these toxins in fish can lead to CP. To date, there is no unique validated methodology for their study, and demonstrating their presence in fish tissues is an analytical challenge. The main techniques used are cell-based assay and liquid chromatography, which may present different matrix effect interferences; thus, purification protocols are necessary. Six cleanup strategies for fish extracts, assessing the principal analogues found in fish in different parts of the world (CTX1B/CTX3C/C-CTX1), are compared here. Cleaned-up extracts are evaluated by cell-based assay and chromatography. All protocols are suitable for recovering the analogues of CTXs. Two of them, those that used polystyrene-divinylbenzene and silica cartridges, achieve the most adequate results showing toxicity in their fractions over 53% and chromatography efficiencies over 79% for CTX1B/CTX3C, proving to be the most versatile clean-ups for the study of the different CTX analogues. |
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| ISSN: | 1520-5118 |
| DOI: | 10.1021/acs.jafc.5c01142 |