Blood total calcium concentrations in the fourth week postpartum of dairy cows continue to play a key role in uterine involution.
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| Title: | Blood total calcium concentrations in the fourth week postpartum of dairy cows continue to play a key role in uterine involution. |
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| Authors: | Tobolski D; Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787, Warsaw, Poland. Electronic address: dawid_tobolski@sggw.edu.pl., Pascottini OB; School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland., Opsomer G; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium., Tobolska A; Department of Surgery and Radiology with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14, 10-719, Olsztyn, Poland., Barański W; Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14, 10-719, Olsztyn, Poland. |
| Source: | Theriogenology [Theriogenology] 2025 Oct 01; Vol. 245, pp. 117486. Date of Electronic Publication: 2025 May 22. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| Imprint Name(s): | Publication: [New York, N.Y.?] : Elsevier Original Publication: Los Altos, Calif., Geron-X. |
| MeSH Terms: | Postpartum Period*/blood , Postpartum Period*/physiology , Calcium*/blood , Uterus*/physiology , Uterus*/anatomy & histology, Animals ; Female ; Cattle/blood ; Cattle/physiology ; Cattle Diseases/blood ; Endometritis/veterinary ; Endometritis/blood |
| Abstract: | Competing Interests: Conflict of interest statement The authors declare that they have no conflicts of interest regarding the publication of this manuscript, titled "Blood total calcium concentrations in the fourth week postpartum of dairy cows continue to play a key role in uterine involution." All authors have contributed to this manuscript according to their roles and responsibilities, and no personal, professional, or financial relationships exist that could inappropriately influence the research presented in this study. The study was conducted independently, and the data collection, analysis, and interpretation were carried out without any influence from external funding sources or third parties. Signed by all authors as follows: Dawid Tobolski, Osvaldo Bogado Pascottini, Geert Opsomer, Angelika Tobolska, Wojciech Barański. In the present study, we examined the relationship between uterine involution, serum metabolites and calcium concentrations in clinically healthy postpartum dairy cows with or without subclinical endometritis (SCE). A cohort of 43 Holstein cows, sampled between 21 and 29 days postpartum, was selected based on the absence of clinical endometritis or other clinical postparturient diseases. Reproductive tract measurements included the diameters of the cervix and uterine horns, and blood sample analyses included serum total calcium, total cholesterol, triglycerides, non-esterified fatty acids, and β-hydroxybutyrate. Using the cytobrush, endometrial cytology samples were collected to assess the proportion of endometrial polymorphonuclear leukocytes (PMNs) at 21-29 days postpartum. Results indicated no association between SCE, defined as >5 % PMNs in endometrial smears, and either uterine dimensions or serum calcium levels. However, a general linear model, accounting for random effects of parity and days postpartum, demonstrated that lower serum calcium concentrations were associated with greater cervical and uterine horn diameters (P < 0.001), suggesting a linear dependence of uterine structural dimensions on calcium concentration. For all the other metabolites, no associations were found with cervical nor uterine horns diameters. This indicates blood calcium's role in supporting uterine involution during the late postpartum period. Interestingly, the PMN% in endometrial samples (characteristic of SCE) did not correlate with changes in uterine size, suggesting that other factors related to the periparturient period rather than serum calcium levels influence the development of SCE in the fourth week postpartum. (Copyright © 2025 Elsevier Inc. All rights reserved.) |
| Contributed Indexing: | Keywords: Metabolic stress; Postpartum period; Subclinical endometritis; Subclinical hypocalcemia; Uterine health |
| Substance Nomenclature: | SY7Q814VUP (Calcium) |
| Entry Date(s): | Date Created: 20250529 Date Completed: 20250624 Latest Revision: 20250624 |
| Update Code: | 20250625 |
| DOI: | 10.1016/j.theriogenology.2025.117486 |
| PMID: | 40440861 |
| Database: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | Competing Interests: Conflict of interest statement The authors declare that they have no conflicts of interest regarding the publication of this manuscript, titled "Blood total calcium concentrations in the fourth week postpartum of dairy cows continue to play a key role in uterine involution." All authors have contributed to this manuscript according to their roles and responsibilities, and no personal, professional, or financial relationships exist that could inappropriately influence the research presented in this study. The study was conducted independently, and the data collection, analysis, and interpretation were carried out without any influence from external funding sources or third parties. Signed by all authors as follows: Dawid Tobolski, Osvaldo Bogado Pascottini, Geert Opsomer, Angelika Tobolska, Wojciech Barański.<br />In the present study, we examined the relationship between uterine involution, serum metabolites and calcium concentrations in clinically healthy postpartum dairy cows with or without subclinical endometritis (SCE). A cohort of 43 Holstein cows, sampled between 21 and 29 days postpartum, was selected based on the absence of clinical endometritis or other clinical postparturient diseases. Reproductive tract measurements included the diameters of the cervix and uterine horns, and blood sample analyses included serum total calcium, total cholesterol, triglycerides, non-esterified fatty acids, and β-hydroxybutyrate. Using the cytobrush, endometrial cytology samples were collected to assess the proportion of endometrial polymorphonuclear leukocytes (PMNs) at 21-29 days postpartum. Results indicated no association between SCE, defined as >5 % PMNs in endometrial smears, and either uterine dimensions or serum calcium levels. However, a general linear model, accounting for random effects of parity and days postpartum, demonstrated that lower serum calcium concentrations were associated with greater cervical and uterine horn diameters (P < 0.001), suggesting a linear dependence of uterine structural dimensions on calcium concentration. For all the other metabolites, no associations were found with cervical nor uterine horns diameters. This indicates blood calcium's role in supporting uterine involution during the late postpartum period. Interestingly, the PMN% in endometrial samples (characteristic of SCE) did not correlate with changes in uterine size, suggesting that other factors related to the periparturient period rather than serum calcium levels influence the development of SCE in the fourth week postpartum.<br /> (Copyright © 2025 Elsevier Inc. All rights reserved.) |
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| ISSN: | 1879-3231 |
| DOI: | 10.1016/j.theriogenology.2025.117486 |