Muscle regeneration is improved by hot water immersion but unchanged by cold following a simulated musculoskeletal injury in humans.
Uložené v:
| Názov: | Muscle regeneration is improved by hot water immersion but unchanged by cold following a simulated musculoskeletal injury in humans. |
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| Autori: | Dablainville V; Aspetar Orthopaedic and Sports Medicine Hospital, Research and Scientific Support Department, Doha, Qatar.; DMEM, University of Montpellier, INRAE, Montpellier, France., Mornas A; French Institute of Sport (INSEP), Laboratory Sport, Expertise and Performance (EA 7370), Paris, France.; University Paris Cité, Paris, France.; Montreal Heart Institute, Montréal, Quebec, Canada., Normand-Gravier T; DMEM, University of Montpellier, INRAE, Montpellier, France.; Faculty of Sports Sciences, Laboratoire interdisciplinaire Performance Santé Environnement de Montagne (LIPSEM), UR4640, University of Perpignan Via Domitia (UPVD), Font-Romeu, France., Al-Mulla M; Aspetar Orthopaedic and Sports Medicine Hospital, Research and Scientific Support Department, Doha, Qatar., Papakostas E; Aspetar Orthopaedic and Sports Medicine Hospital, Surgery Department, Doha, Qatar., Olory B; Aspetar Orthopaedic and Sports Medicine Hospital, Surgery Department, Doha, Qatar., Fermin TM; Aspetar Orthopaedic and Sports Medicine Hospital, Surgery Department, Doha, Qatar.; Centro Médico Profesional Las Mercedes, Caracas, Venezuela., Zampeli F; Aspetar Orthopaedic and Sports Medicine Hospital, Surgery Department, Doha, Qatar.; Hand-Upper Limb-Microsurgery Department, General Hospital KAT, Kifisia, Greece., Nader N; Aspetar Orthopaedic and Sports Medicine Hospital, Research and Scientific Support Department, Doha, Qatar., Alhammoud M; Aspetar Orthopaedic and Sports Medicine Hospital, Surgery Department, Doha, Qatar.; Inter-University Laboratory of Human Movement Biology (EA 7424), Savoie Mont Blanc University, Chambéry, France., Bayne F; Sport and Exercise Science Research Centre, School of Applied Sciences, London South Bank University, London, UK., Sanchez AMJ; Faculty of Sports Sciences, Laboratoire interdisciplinaire Performance Santé Environnement de Montagne (LIPSEM), UR4640, University of Perpignan Via Domitia (UPVD), Font-Romeu, France., Cardinale M; Aspetar Orthopaedic and Sports Medicine Hospital, Research and Scientific Support Department, Doha, Qatar.; Institute of Sport, Exercise and Health, University College London, London, UK.; Department of Sport, Exercise and Rehabilitation, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, UK., Candau R; DMEM, University of Montpellier, INRAE, Montpellier, France., Bernardi H; DMEM, University of Montpellier, INRAE, Montpellier, France., Racinais S; Aspetar Orthopaedic and Sports Medicine Hospital, Research and Scientific Support Department, Doha, Qatar.; DMEM, University of Montpellier, INRAE, Montpellier, France.; French Institute of Sport (INSEP), Laboratory Sport, Expertise and Performance (EA 7370), Paris, France.; CREPS Montpellier Font-Romeu, Environmental Stress Unit, Montpellier, France. |
| Zdroj: | The Journal of physiology [J Physiol] 2025 Dec; Vol. 603 (23), pp. 7603-7625. Date of Electronic Publication: 2025 May 28. |
| Spôsob vydávania: | Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: Cambridge Univ. Press Country of Publication: England NLM ID: 0266262 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-7793 (Electronic) Linking ISSN: 00223751 NLM ISO Abbreviation: J Physiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Oxford : Blackwell : Cambridge Univ. Press Original Publication: London, Cambridge Univ. Press. |
| Výrazy zo slovníka MeSH: | Muscle, Skeletal*/physiology , Muscle, Skeletal*/injuries , Regeneration* , Hot Temperature* , Cold Temperature* , Cryotherapy*/methods, Humans ; Male ; Female ; Adult ; Immersion ; Young Adult ; Water ; Creatine Kinase/blood ; Myoglobin/blood |
| Abstrakt: | Cryotherapy is a popular strategy for the treatment of skeletal muscle injuries. However, its effect on post-injury human muscle regeneration remains unclear. In contrast, promising results recently emerged using heat therapy to facilitate recovery from muscle injury. This study aimed to examine the effect of three different thermal treatments on muscle recovery and regeneration following a simulated injury in humans. Thirty-four participants underwent a muscle damage protocol induced by electrically stimulated eccentric contractions triggering regenerative processes following myofibre necrosis. Thereafter, participants were exposed to daily lower body water immersion for 10 days in cold (CWI, 15 min at 12°C), thermoneutral (TWI, 30 min at 32°C) or hot water immersion (HWI, 60 min at 42°C). Muscle biopsies were sampled before and at +5 (D5) and +11 (D11) days post-damage. None of the water immersions differed in recovery of force-generating capacity (P = 0.108). HWI induced a lower perceived muscle pain than TWI (P = 0.035) and lower levels of circulating creatine kinase (P ≤ 0.012) and myoglobin (P < 0.001) than TWI and CWI. Contrary to our hypothesis, CWI did not improve perceived muscle pain or reduce circulating markers of muscle damage (P ≥ 0.207). Expression of heat shock proteins 27 and 70 was significantly increased in HWI (P < 0.038) at D11 and appeared blunted using CWI. Furthermore, nuclear factor-κB expression significantly increased in all conditions except HWI, while interleukin-10 was upregulated only in HWI at D11 (P = 0.014). In conclusion, our results support the use of HWI but not cold, to improve muscle regeneration following an injury. KEY POINTS: Cryotherapy and heat therapy are popular strategies in the treatment of skeletal muscle injury; however, existing literature is equivocal, and their effects on human muscle regeneration remain unknown. We investigated the effect of three thermal treatments (cold water immersion (CWI): 15 min at 12°C; thermoneutral water immersion (TWI): 30 min at 32°C; or hot water immersion (HWI): 60 min at 42°C) performed daily for 10 days following electrically stimulated eccentric muscle damage inducing regenerative mechanisms. CWI did not improve chronic perceived muscle pain nor reduce circulating markers of muscle damage. HWI limited chronic perceived pain and circulating markers of muscle damage, potentially influenced inflammatory mechanisms, and increased the expression of heat shock proteins. HWI appears more beneficial than CWI in improving muscle regeneration after a muscle injury. (© 2025 The Author(s). The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.) |
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| Grant Information: | 2022 Aspire Zone Foundation |
| Contributed Indexing: | Keywords: CWI; HWI; damage; electrically stimulated eccentric contraction; muscle injury |
| Substance Nomenclature: | 059QF0KO0R (Water) EC 2.7.3.2 (Creatine Kinase) 0 (Myoglobin) |
| Entry Date(s): | Date Created: 20250529 Date Completed: 20251201 Latest Revision: 20251203 |
| Update Code: | 20251203 |
| PubMed Central ID: | PMC12666504 |
| DOI: | 10.1113/JP287777 |
| PMID: | 40437768 |
| Databáza: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Cryotherapy is a popular strategy for the treatment of skeletal muscle injuries. However, its effect on post-injury human muscle regeneration remains unclear. In contrast, promising results recently emerged using heat therapy to facilitate recovery from muscle injury. This study aimed to examine the effect of three different thermal treatments on muscle recovery and regeneration following a simulated injury in humans. Thirty-four participants underwent a muscle damage protocol induced by electrically stimulated eccentric contractions triggering regenerative processes following myofibre necrosis. Thereafter, participants were exposed to daily lower body water immersion for 10 days in cold (CWI, 15 min at 12°C), thermoneutral (TWI, 30 min at 32°C) or hot water immersion (HWI, 60 min at 42°C). Muscle biopsies were sampled before and at +5 (D5) and +11 (D11) days post-damage. None of the water immersions differed in recovery of force-generating capacity (P = 0.108). HWI induced a lower perceived muscle pain than TWI (P = 0.035) and lower levels of circulating creatine kinase (P ≤ 0.012) and myoglobin (P < 0.001) than TWI and CWI. Contrary to our hypothesis, CWI did not improve perceived muscle pain or reduce circulating markers of muscle damage (P ≥ 0.207). Expression of heat shock proteins 27 and 70 was significantly increased in HWI (P < 0.038) at D11 and appeared blunted using CWI. Furthermore, nuclear factor-κB expression significantly increased in all conditions except HWI, while interleukin-10 was upregulated only in HWI at D11 (P = 0.014). In conclusion, our results support the use of HWI but not cold, to improve muscle regeneration following an injury. KEY POINTS: Cryotherapy and heat therapy are popular strategies in the treatment of skeletal muscle injury; however, existing literature is equivocal, and their effects on human muscle regeneration remain unknown. We investigated the effect of three thermal treatments (cold water immersion (CWI): 15 min at 12°C; thermoneutral water immersion (TWI): 30 min at 32°C; or hot water immersion (HWI): 60 min at 42°C) performed daily for 10 days following electrically stimulated eccentric muscle damage inducing regenerative mechanisms. CWI did not improve chronic perceived muscle pain nor reduce circulating markers of muscle damage. HWI limited chronic perceived pain and circulating markers of muscle damage, potentially influenced inflammatory mechanisms, and increased the expression of heat shock proteins. HWI appears more beneficial than CWI in improving muscle regeneration after a muscle injury.<br /> (© 2025 The Author(s). The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.) |
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| ISSN: | 1469-7793 |
| DOI: | 10.1113/JP287777 |