Gonadotropin inhibitory hormone (GnIH) induces glucose metabolism disorders and ovarian dysfunction in ovarian white follicles of hens.
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| Titel: | Gonadotropin inhibitory hormone (GnIH) induces glucose metabolism disorders and ovarian dysfunction in ovarian white follicles of hens. |
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| Autoren: | Shen B; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Han D; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Xin Y; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Zhang J; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Xu C; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Song X; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Zhang X; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Liu C; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Li J; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Wei Y; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Wang X; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Hu C; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China., Li X; College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, Guangxi, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China. Electronic address: xunli@gxu.edu.cn. |
| Quelle: | Theriogenology [Theriogenology] 2025 Sep 15; Vol. 244, pp. 117497. Date of Electronic Publication: 2025 May 16. |
| Publikationsart: | Journal Article |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| Imprint Name(s): | Publication: [New York, N.Y.?] : Elsevier Original Publication: Los Altos, Calif., Geron-X. |
| MeSH-Schlagworte: | Chickens* , Ovarian Follicle*/drug effects , Ovarian Follicle*/metabolism , Hypothalamic Hormones*/metabolism , Hypothalamic Hormones*/pharmacology , Hypothalamic Hormones*/genetics , Glucose Metabolism Disorders*/chemically induced , Glucose Metabolism Disorders*/veterinary , Ovarian Diseases*/veterinary , Ovarian Diseases*/chemically induced, Animals ; Female ; Glucose/metabolism |
| Abstract: | Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Energy metabolism is crucial for reproduction, and disturbances in glucose metabolism are closely associated with reproductive disorders. The gonadotropin inhibitory hormone (GnIH) plays a crucial role in reproduction and glucose homeostasis in both birds and mammals. However, its specific effects on glucose metabolism-associated ovarian dysfunction in hens remain uncharacterized. In this study, we investigated the effects of GnIH on ovarian function and glucose homeostasis in hens using combined in vivo and in vitro approaches. Our results showed that GnIH and its receptor, GPR147, are predominantly expressed in white follicles. Continuous GnIH injection significantly reduced the ovarian index and the number of prehierarchical follicles, concurrently suppressing Fshr and LHr expression in white follicles. Additionally, metabolomic analyses indicate changes in key glucose metabolism pathways, indicating a regulatory role of GnIH in glucose metabolism. To validate our metabolomics findings, the effects of GnIH on glucose homeostasis in ovarian white follicles and granulosa cells (GCs) were further investigated through in vivo and in vitro studies. The results demonstrated that GnIH enhanced glucose transport, glycolysis and glycogen synthesis, but concurrently induced insulin resistance, oxidative stress, and mitochondrial dysfunction, ultimately leading to reduced energy levels and apoptosis in ovarian tissues. In summary, our study reveals that GnIH contributes to ovarian dysfunction via glucose metabolism dysregulation in hens, suggesting its potential as a therapeutic target for metabolic disorder-associated ovarian impairment in chickens. (Copyright © 2025 Elsevier Inc. All rights reserved.) |
| Contributed Indexing: | Keywords: Glucose metabolism; Gonadotropin-inhibitory hormone (GnIH); Mitochondria; Ovarian function |
| Substance Nomenclature: | 0 (Hypothalamic Hormones) IY9XDZ35W2 (Glucose) |
| Entry Date(s): | Date Created: 20250523 Date Completed: 20250604 Latest Revision: 20250604 |
| Update Code: | 20250605 |
| DOI: | 10.1016/j.theriogenology.2025.117497 |
| PMID: | 40408803 |
| Datenbank: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br />Energy metabolism is crucial for reproduction, and disturbances in glucose metabolism are closely associated with reproductive disorders. The gonadotropin inhibitory hormone (GnIH) plays a crucial role in reproduction and glucose homeostasis in both birds and mammals. However, its specific effects on glucose metabolism-associated ovarian dysfunction in hens remain uncharacterized. In this study, we investigated the effects of GnIH on ovarian function and glucose homeostasis in hens using combined in vivo and in vitro approaches. Our results showed that GnIH and its receptor, GPR147, are predominantly expressed in white follicles. Continuous GnIH injection significantly reduced the ovarian index and the number of prehierarchical follicles, concurrently suppressing Fshr and LHr expression in white follicles. Additionally, metabolomic analyses indicate changes in key glucose metabolism pathways, indicating a regulatory role of GnIH in glucose metabolism. To validate our metabolomics findings, the effects of GnIH on glucose homeostasis in ovarian white follicles and granulosa cells (GCs) were further investigated through in vivo and in vitro studies. The results demonstrated that GnIH enhanced glucose transport, glycolysis and glycogen synthesis, but concurrently induced insulin resistance, oxidative stress, and mitochondrial dysfunction, ultimately leading to reduced energy levels and apoptosis in ovarian tissues. In summary, our study reveals that GnIH contributes to ovarian dysfunction via glucose metabolism dysregulation in hens, suggesting its potential as a therapeutic target for metabolic disorder-associated ovarian impairment in chickens.<br /> (Copyright © 2025 Elsevier Inc. All rights reserved.) |
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| ISSN: | 1879-3231 |
| DOI: | 10.1016/j.theriogenology.2025.117497 |