Effect of Selcopintide on Periodontal Tissue Regeneration in Chronic One-Wall Intrabony Defect Model.
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| Název: | Effect of Selcopintide on Periodontal Tissue Regeneration in Chronic One-Wall Intrabony Defect Model. |
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| Autoři: | Ko YC; Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea., Lee DS; Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology and Developmental Biology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.; Regenerative Dental Medicine R and D Center, HysensBio Co. Ltd, Gwacheon-si, Gyeonggi-do, Republic of Korea., Seol YJ; Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea., Lee YM; Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea., Koo KT; Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea., Park JC; Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology and Developmental Biology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.; Regenerative Dental Medicine R and D Center, HysensBio Co. Ltd, Gwacheon-si, Gyeonggi-do, Republic of Korea., Shon WJ; Department of Conservative Dentistry, School of Dentistry and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Republic of Korea., Lee J; Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.; One-Stop Specialty Center, Seoul National University Dental Hospital, Seoul, Republic of Korea. |
| Zdroj: | Journal of periodontal research [J Periodontal Res] 2025 Aug; Vol. 60 (8), pp. 835-846. Date of Electronic Publication: 2025 Mar 19. |
| Způsob vydávání: | Journal Article |
| Jazyk: | English |
| Informace o časopise: | Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 0055107 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0765 (Electronic) Linking ISSN: 00223484 NLM ISO Abbreviation: J Periodontal Res Subsets: MEDLINE |
| Imprint Name(s): | Publication: Malden, MA : Wiley-Blackwell Original Publication: Copenhagen : Munksgaard International Publishers |
| Výrazy ze slovníku MeSH: | Guided Tissue Regeneration, Periodontal*/methods , Regeneration*/drug effects , Periodontium*/drug effects , Alveolar Bone Loss*/surgery, Animals ; Dogs ; Periodontal Ligament/drug effects ; Periodontal Ligament/cytology ; Cell Differentiation/drug effects ; Dental Cementum/drug effects ; Disease Models, Animal ; Osteoblasts/drug effects ; Bone Regeneration/drug effects ; Cell Adhesion Molecules ; Integrin-Binding Sialoprotein ; Extracellular Matrix Proteins |
| Abstrakt: | Aims: Periodontal diseases pose significant challenges to oral health, making the regeneration of periodontal tissues a critical therapeutic goal. The goal is to restore dental function by repairing damaged tissue and reconstructing the healthy connective structure between the teeth and the alveolar bone. This study aimed to investigate the effects of selcopintide (SCPT) on the differentiation of periodontal ligament cells (PDLCs), cementoblasts, and osteoblasts in vitro, as well as the regeneration of periodontal tissue using a periodontal tissue defect model in dogs in vivo. Methods: The expression of periodontal tissue marker genes, including periostin (POSTN), cementum attachment protein (CAP), dentin matrix protein 1 (DMP1), and bone sialoprotein (BSP), was investigated in vitro. Chronic one-wall intrabony defects were created in a total of 12 beagle dogs (n = 6 at 6 and 12 weeks, respectively), and the surgical sites were treated with no treatment, guided tissue regeneration (GTR), GTR with SCPT 50 μg/0.1 mL, 100 μg/0.1 mL, and 250 μg/0.1 mL. The effects of SCPT on the regeneration of periodontal tissues, such as periodontal ligament (PDL), cementum, and bone, were analyzed in vivo. Results: SCPT influenced the proliferation and differentiation of cementoblasts and PDLCs. Real-time polymerase chain reaction analysis showed that SCPT upregulated the expression of POSTN, CAP, DMP1, and BSP compared to the control. In the periodontal defect model, SCPT regenerated the periodontal complex. Additionally, the arrangement of the newly formed PDL-like fibers was perpendicular to the newly formed cementum and alveolar bone, similar to Sharpey's fibers in natural teeth, compared with the control. Conclusion: In this preclinical study, histological and immunohistochemical analyses suggest that GTR with SCPT might be associated with increased periodontal ligament attachment and enhanced cementum and alveolar bone formation. Additional research with a larger sample size is needed to establish the optimal therapeutic protocols and validate the regenerative potential of SCPT. (© 2025 The Author(s). Journal of Periodontal Research published by John Wiley & Sons Ltd.) |
| References: | J Tissue Eng Regen Med. 2021 Nov;15(11):964-997. (PMID: 34480421) J Periodontal Res. 2017 Dec;52(6):965-974. (PMID: 28635007) J Clin Periodontol. 2021 Jan;48(1):100-113. (PMID: 33025619) J Periodontol. 2004 Feb;75(2):229-35. (PMID: 15068110) J Clin Periodontol. 2022 Jun;49(6):609-620. (PMID: 35373365) Regen Ther. 2023 Jan 24;22:128-135. (PMID: 36760990) J Clin Periodontol. 2010 Jun;37(6):534-43. (PMID: 20507377) J Periodontal Implant Sci. 2024 Dec;54(6):393-404. (PMID: 38725429) J Clin Periodontol. 1994 Mar;21(3):189-93. (PMID: 8157772) Front Immunol. 2023 Apr 11;14:1151322. (PMID: 37114060) J Periodontal Res. 2002 Oct;37(5):380-8. (PMID: 12366862) Periodontol 2000. 2018 Oct;78(1):212-226. (PMID: 30198128) Materials (Basel). 2020 Oct 16;13(20):. (PMID: 33081300) Genesis. 2022 Sep;60(8-9):e23499. (PMID: 36086991) Front Bioeng Biotechnol. 2022 Aug 26;10:961898. (PMID: 36091440) J Clin Periodontol. 2023 May;50(5):604-626. (PMID: 36631982) J Periodontol. 2008 Aug;79(8):1480-90. (PMID: 18672999) J Periodontol. 2024 Oct;95(10):977-990. (PMID: 38865252) J Periodontal Implant Sci. 2023 Dec;53(6):406-416. (PMID: 37154106) Medicina (Kaunas). 2024 May 07;60(5):. (PMID: 38792956) Nanomaterials (Basel). 2022 Nov 02;12(21):. (PMID: 36364654) J Periodontal Implant Sci. 2024 Jun;54(3):149-160. (PMID: 37857519) J Periodontal Implant Sci. 2011 Dec;41(6):285-92. (PMID: 22324006) J Periodontol. 2023 Aug;94(8):1032-1044. (PMID: 36799425) Periodontol 2000. 2020 Jun;83(1):7-13. (PMID: 32385880) J Clin Periodontol. 2017 May;44(5):456-462. (PMID: 28419559) Arch Oral Biol. 2022 Nov;143:105531. (PMID: 36063644) Periodontol 2000. 2006;41:30-47. (PMID: 16686925) PLoS Biol. 2020 Jul 14;18(7):e3000410. (PMID: 32663219) |
| Grant Information: | Ministry of Health & Welfare, Republic of Korea |
| Contributed Indexing: | Keywords: COPINE‐7; bone regeneration; cementogenesis; periodontal ligament regeneration; selcopintide |
| Substance Nomenclature: | 0 (Cell Adhesion Molecules) 0 (Integrin-Binding Sialoprotein) 0 (Extracellular Matrix Proteins) |
| Entry Date(s): | Date Created: 20250319 Date Completed: 20250927 Latest Revision: 20250929 |
| Update Code: | 20250929 |
| PubMed Central ID: | PMC12476083 |
| DOI: | 10.1111/jre.13395 |
| PMID: | 40105181 |
| Databáze: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Aims: Periodontal diseases pose significant challenges to oral health, making the regeneration of periodontal tissues a critical therapeutic goal. The goal is to restore dental function by repairing damaged tissue and reconstructing the healthy connective structure between the teeth and the alveolar bone. This study aimed to investigate the effects of selcopintide (SCPT) on the differentiation of periodontal ligament cells (PDLCs), cementoblasts, and osteoblasts in vitro, as well as the regeneration of periodontal tissue using a periodontal tissue defect model in dogs in vivo.<br />Methods: The expression of periodontal tissue marker genes, including periostin (POSTN), cementum attachment protein (CAP), dentin matrix protein 1 (DMP1), and bone sialoprotein (BSP), was investigated in vitro. Chronic one-wall intrabony defects were created in a total of 12 beagle dogs (n = 6 at 6 and 12 weeks, respectively), and the surgical sites were treated with no treatment, guided tissue regeneration (GTR), GTR with SCPT 50 μg/0.1 mL, 100 μg/0.1 mL, and 250 μg/0.1 mL. The effects of SCPT on the regeneration of periodontal tissues, such as periodontal ligament (PDL), cementum, and bone, were analyzed in vivo.<br />Results: SCPT influenced the proliferation and differentiation of cementoblasts and PDLCs. Real-time polymerase chain reaction analysis showed that SCPT upregulated the expression of POSTN, CAP, DMP1, and BSP compared to the control. In the periodontal defect model, SCPT regenerated the periodontal complex. Additionally, the arrangement of the newly formed PDL-like fibers was perpendicular to the newly formed cementum and alveolar bone, similar to Sharpey's fibers in natural teeth, compared with the control.<br />Conclusion: In this preclinical study, histological and immunohistochemical analyses suggest that GTR with SCPT might be associated with increased periodontal ligament attachment and enhanced cementum and alveolar bone formation. Additional research with a larger sample size is needed to establish the optimal therapeutic protocols and validate the regenerative potential of SCPT.<br /> (© 2025 The Author(s). Journal of Periodontal Research published by John Wiley & Sons Ltd.) |
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| ISSN: | 1600-0765 |
| DOI: | 10.1111/jre.13395 |