Development and Optimization of a Cost-Effective Electrochemical Immunosensor for Rapid COVID-19 Diagnosis.
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| Názov: | Development and Optimization of a Cost-Effective Electrochemical Immunosensor for Rapid COVID-19 Diagnosis. |
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| Autori: | Lima TM; Institute of Science and Technology, Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39100-000, Minas Gerais, Brazil., Leal DM; Institute of Science and Technology, Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39100-000, Minas Gerais, Brazil., Ferreira ZC; Institute of Science and Technology, Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39100-000, Minas Gerais, Brazil., Souza FJ; Faculty of Medicine, Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39100-000, Minas Gerais, Brazil., de Oliveira DB; Faculty of Medicine, Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39100-000, Minas Gerais, Brazil., Rocha-Vieira E; Faculty of Medicine, Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39100-000, Minas Gerais, Brazil., Martins HR; Pharmacy Department, Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39100-000, Minas Gerais, Brazil., Pereira AC; Department of Natural Sciences, Federal University of São João del-Rei (UFSJ), São João del-Rei 36307-352, Minas Gerais, Brazil., Ferreira LF; Institute of Science and Technology, Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39100-000, Minas Gerais, Brazil. |
| Zdroj: | Biosensors [Biosensors (Basel)] 2025 Jan 22; Vol. 15 (2). Date of Electronic Publication: 2025 Jan 22. |
| Spôsob vydávania: | Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI Pub. |
| Výrazy zo slovníka MeSH: | Biosensing Techniques*/economics , Biosensing Techniques*/instrumentation , Biosensing Techniques*/methods , Biosensing Techniques*/standards , Electrochemistry*/economics , Electrochemistry*/instrumentation , Electrochemistry*/methods , Rapid Diagnostic Tests*/economics , Rapid Diagnostic Tests*/instrumentation , Rapid Diagnostic Tests*/methods , Rapid Diagnostic Tests*/standards , COVID-19*/diagnosis, Sensitivity and Specificity ; Antibodies, Immobilized ; Mucus/virology ; Humans ; Metal Nanoparticles/chemistry ; Graphite/chemistry ; SARS-CoV-2 ; Reproducibility of Results |
| Abstrakt: | The coronavirus disease (COVID-19) pandemic has created an urgent need for rapid, accurate, and cost-effective diagnostic tools. In this study, an economical electrochemical immunosensor for the rapid diagnosis of COVID-19 was developed and optimized based on charge transfer resistance (Rct) values obtained by electrochemical impedance spectroscopy (EIS) from the interaction between antibodies (anti-SARS-CoV-2) immobilized as a bioreceptor and the virus (SARS-CoV-2). The sensor uses modified pencil graphite electrodes (PGE) coated with poly(4-hydroxybenzoic acid), anti-SARS-CoV-2, and silver nanoparticles. The immobilization of anti-SARS-CoV-2 antibodies was optimized at a concentration of 1:250 for 30 min, followed by blocking the surface with 0.01% bovine serum albumin for 10 min. The optimal conditions for virus detection in clinical samples were a 1:10 dilution with a response time of 20 min. The immunosensor responded linearly in the range of 0.2-2.5 × 10 6 particles/μL. From the relationship between the obtained signal and the concentration of the analyzed sample, the limit of detection (LOD) and limit of quantification (LOQ) obtained were 1.21 × 10 6 and 4.04 × 10 6 particles/μL, respectively. The device did not cross-react with other viruses, including Influenza A and B, HIV, and Vaccinia virus. The relative standard deviation (RSD) of the six immunosensors prepared using the shared-pool sample was 3.87. Decreases of 22.3% and 12.4% were observed in the response values of the ten immunosensors stored at 25 °C and 4.0 °C, respectively. The sensor provides timely and accurate results with high sensitivity and specificity, offering a cost-effective alternative to the existing diagnostic methods. |
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| Contributed Indexing: | Keywords: 4-hydroxybenzoic acid; SARS-CoV-2; electrochemical platforms; electropolymerization; pencil graphite electrodes |
| Substance Nomenclature: | 0 (Antibodies, Immobilized) 7782-42-5 (Graphite) |
| Entry Date(s): | Date Created: 20250225 Date Completed: 20250226 Latest Revision: 20250227 |
| Update Code: | 20250227 |
| PubMed Central ID: | PMC11853419 |
| DOI: | 10.3390/bios15020067 |
| PMID: | 39996968 |
| Databáza: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The coronavirus disease (COVID-19) pandemic has created an urgent need for rapid, accurate, and cost-effective diagnostic tools. In this study, an economical electrochemical immunosensor for the rapid diagnosis of COVID-19 was developed and optimized based on charge transfer resistance (Rct) values obtained by electrochemical impedance spectroscopy (EIS) from the interaction between antibodies (anti-SARS-CoV-2) immobilized as a bioreceptor and the virus (SARS-CoV-2). The sensor uses modified pencil graphite electrodes (PGE) coated with poly(4-hydroxybenzoic acid), anti-SARS-CoV-2, and silver nanoparticles. The immobilization of anti-SARS-CoV-2 antibodies was optimized at a concentration of 1:250 for 30 min, followed by blocking the surface with 0.01% bovine serum albumin for 10 min. The optimal conditions for virus detection in clinical samples were a 1:10 dilution with a response time of 20 min. The immunosensor responded linearly in the range of 0.2-2.5 × 10 <sup>6</sup> particles/μL. From the relationship between the obtained signal and the concentration of the analyzed sample, the limit of detection (LOD) and limit of quantification (LOQ) obtained were 1.21 × 10 <sup>6</sup> and 4.04 × 10 <sup>6</sup> particles/μL, respectively. The device did not cross-react with other viruses, including Influenza A and B, HIV, and Vaccinia virus. The relative standard deviation (RSD) of the six immunosensors prepared using the shared-pool sample was 3.87. Decreases of 22.3% and 12.4% were observed in the response values of the ten immunosensors stored at 25 °C and 4.0 °C, respectively. The sensor provides timely and accurate results with high sensitivity and specificity, offering a cost-effective alternative to the existing diagnostic methods. |
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| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios15020067 |