CA1 ensembles expressing immediate-early genes are driven by context switch, shrink with sustained presence, and show no effect of change of task demands.
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| Titel: | CA1 ensembles expressing immediate-early genes are driven by context switch, shrink with sustained presence, and show no effect of change of task demands. |
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| Autoren: | Krajcovic B; Laboratory of Neurophysiology of Memory, Institute of Physiology, Czech Academy of Sciences, Prague, Czechia; Third Faculty of Medicine, Charles University, Prague, Czechia. Electronic address: branislav.krajcovic@lfmotol.cuni.cz., Cernotova D; Laboratory of Neurophysiology of Memory, Institute of Physiology, Czech Academy of Sciences, Prague, Czechia; Third Faculty of Medicine, Charles University, Prague, Czechia., Buchtova H; Laboratory of Neurophysiology of Memory, Institute of Physiology, Czech Academy of Sciences, Prague, Czechia; Third Faculty of Medicine, Charles University, Prague, Czechia., Stuchlik A; Laboratory of Neurophysiology of Memory, Institute of Physiology, Czech Academy of Sciences, Prague, Czechia., Kubik S; Laboratory of Neurophysiology of Memory, Institute of Physiology, Czech Academy of Sciences, Prague, Czechia., Svoboda J; Laboratory of Neurophysiology of Memory, Institute of Physiology, Czech Academy of Sciences, Prague, Czechia. |
| Quelle: | Behavioural brain research [Behav Brain Res] 2025 Mar 05; Vol. 480, pp. 115407. Date of Electronic Publication: 2024 Dec 20. |
| Publikationsart: | Journal Article; Research Support, Non-U.S. Gov't |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8004872 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7549 (Electronic) Linking ISSN: 01664328 NLM ISO Abbreviation: Behav Brain Res Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| MeSH-Schlagworte: | CA1 Region, Hippocampal*/metabolism , CA1 Region, Hippocampal*/physiology , Homer Scaffolding Proteins*/metabolism , Homer Scaffolding Proteins*/genetics , Genes, Immediate-Early*/physiology , Rats, Long-Evans*, Animals ; Male ; Rats ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Cytoskeletal Proteins/genetics ; Cytoskeletal Proteins/metabolism ; Behavior, Animal/physiology |
| Abstract: | Competing Interests: Declaration of Interest Authors have no conflict of interest to declare. The hippocampus (HPC) is essential for navigation and memory, tracking environmental continuity and change, including navigation relative to moving targets. CA1 ensembles expressing immediate-early gene (IEG) Arc and Homer1a RNA are contextually specific. While IEG expression correlates with HPC-dependent task demands, the effects of behavioral demands on IEG-expressing ensembles remain unclear. In three experiments, we investigated the effects of context switch, sustained presence, and task demands on dorso-proximal CA1 IEG+ ensembles in rats. Experiment 1 showed that the size of IEG+ (Arc, Homer1a RNA) ensembles dropped to baseline during uninterrupted 30-min exploration, reflecting familiarization, unless a context switch was present. Context-specificity of the ensembles depended on both environment identity and timing of the context switch. Experiment 2 found no effect of HPC-dependent mobile robot avoidance or HPC-independent avoidance of a stationary robot on IEG+ ensembles beyond mere exploration. Experiment 3 replicated these findings for c-Fos protein. The data suggest that IEG+ ensembles are driven by a context switch and shrink over time during sustained presence in the same environment. We found no evidence of task demands or their change affecting the size, stability over time, or task-specificity of IEG+ ensembles. These results shed light on the temporal dynamics of CA1 IEG+ ensembles, and their control by contextual and behavioral factors. (Copyright © 2024. Published by Elsevier B.V.) |
| Contributed Indexing: | Keywords: Arc/Homer1a catFISH; Behavioral episode; C-Fos; Ensemble stability; Moving/stationary robot avoidance; RNA |
| Substance Nomenclature: | 0 (Homer Scaffolding Proteins) 0 (Homer1 protein, rat) 0 (activity regulated cytoskeletal-associated protein) 0 (Nerve Tissue Proteins) 0 (Cytoskeletal Proteins) |
| Entry Date(s): | Date Created: 20241222 Date Completed: 20250109 Latest Revision: 20250416 |
| Update Code: | 20250416 |
| DOI: | 10.1016/j.bbr.2024.115407 |
| PMID: | 39710210 |
| Datenbank: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | Competing Interests: Declaration of Interest Authors have no conflict of interest to declare.<br />The hippocampus (HPC) is essential for navigation and memory, tracking environmental continuity and change, including navigation relative to moving targets. CA1 ensembles expressing immediate-early gene (IEG) Arc and Homer1a RNA are contextually specific. While IEG expression correlates with HPC-dependent task demands, the effects of behavioral demands on IEG-expressing ensembles remain unclear. In three experiments, we investigated the effects of context switch, sustained presence, and task demands on dorso-proximal CA1 IEG+ ensembles in rats. Experiment 1 showed that the size of IEG+ (Arc, Homer1a RNA) ensembles dropped to baseline during uninterrupted 30-min exploration, reflecting familiarization, unless a context switch was present. Context-specificity of the ensembles depended on both environment identity and timing of the context switch. Experiment 2 found no effect of HPC-dependent mobile robot avoidance or HPC-independent avoidance of a stationary robot on IEG+ ensembles beyond mere exploration. Experiment 3 replicated these findings for c-Fos protein. The data suggest that IEG+ ensembles are driven by a context switch and shrink over time during sustained presence in the same environment. We found no evidence of task demands or their change affecting the size, stability over time, or task-specificity of IEG+ ensembles. These results shed light on the temporal dynamics of CA1 IEG+ ensembles, and their control by contextual and behavioral factors.<br /> (Copyright © 2024. Published by Elsevier B.V.) |
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| ISSN: | 1872-7549 |
| DOI: | 10.1016/j.bbr.2024.115407 |