The effect of immobilisation strategies on the ability of peptoids to reduce the adhesion of P. aeruginosa strains to contact lenses.
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| Titel: | The effect of immobilisation strategies on the ability of peptoids to reduce the adhesion of P. aeruginosa strains to contact lenses. |
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| Autoren: | Sara M; School of Optometry and Vision Science, UNSW Sydney, Australia. Electronic address: Manjulatha.sara@unsw.edu.au., Chakraborty S; School of Chemistry, UNSW Sydney, Australia. Electronic address: s.chakraborty@unsw.edu.au., Chen R; School of Chemistry, UNSW Sydney, Australia. Electronic address: r.chen@unsw.edu.au., Palms D; Biomedical Nanoengineering Laboratory, College of Medicine and Public Health, Flinders University, Bedford Park, SA, 5042, Australia. Electronic address: dennis.palms@flinders.edu.au., Katsifis G; School of Physics, University of Sydney, NSW, 2006, Australia. Electronic address: gkat2146@uni.sydney.edu.au., Li Z; University of Sydney, Australia. Electronic address: zhongyan.li@sydney.edu.au., Farajikhah S; School of Chemical Engineering, UNSW Sydney, Australia. Electronic address: syamak.farajikhah@sydney.edu.au., Massedupally V; School of Optometry and Vision Science, UNSW Sydney, Australia. Electronic address: vinodm@unsw.edu.au., Hui A; School of Optometry and Vision Science, UNSW Sydney, Australia; Centre for Ocular Research and Education, University of Waterloo, Canada. Electronic address: alex.hui@unsw.edu.au., Wong EHH; School of Optometry and Vision Science, UNSW Sydney, Australia; School of Chemical Engineering, UNSW Sydney, Australia. Electronic address: edgar.wong@unsw.edu.au., Kumar N; School of Chemistry, UNSW Sydney, Australia. Electronic address: n.kumar@unsw.edu.au., Vasilev K; Biomedical Nanoengineering Laboratory, College of Medicine and Public Health, Flinders University, Bedford Park, SA, 5042, Australia. Electronic address: krasimir.vasilev@flinders.edu.au., Mackenzie D; School of Physics, University of Sydney, NSW, 2006, Australia. Electronic address: david.mckenzie@sydney.edu.au., Losurdo L; School of Physics, University of Sydney, NSW, 2006, Australia. Electronic address: linda.losurdo@sydney.edu.au., Dehghani F; University of Sydney, Australia. Electronic address: fariba.dehghani@sydney.edu.au., Jenssen H; Department of Science and Environment, Roskilde University, 4000, Roskilde, Denmark. Electronic address: jenssen@ruc.dk., Sorensen K; Department of Bioengineering, School of Medicine & School of Engineering, Standford University, California, 94305, USA. Electronic address: ksoren@stanford.edu., Lin JS; Department of Bioengineering, School of Medicine & School of Engineering, Standford University, California, 94305, USA. Electronic address: jlin3@stanford.edu., Barron AE; Department of Bioengineering, School of Medicine & School of Engineering, Standford University, California, 94305, USA. Electronic address: aebarron@stanford.edu., Willcox M; School of Optometry and Vision Science, UNSW Sydney, Australia. Electronic address: m.willcox@unsw.edu.au. |
| Quelle: | Experimental eye research [Exp Eye Res] 2025 Jan; Vol. 250, pp. 110149. Date of Electronic Publication: 2024 Nov 20. |
| Publikationsart: | Journal Article |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: Academic Press Country of Publication: England NLM ID: 0370707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0007 (Electronic) Linking ISSN: 00144835 NLM ISO Abbreviation: Exp Eye Res Subsets: MEDLINE |
| Imprint Name(s): | Publication: London : Academic Press Original Publication: London. |
| MeSH-Schlagworte: | Pseudomonas aeruginosa*/drug effects , Bacterial Adhesion*/drug effects , Peptoids*/pharmacology , Contact Lenses, Hydrophilic*/microbiology , Photoelectron Spectroscopy*, Humans ; Eye Infections, Bacterial/prevention & control ; Eye Infections, Bacterial/microbiology ; Epithelium, Corneal/drug effects ; Pseudomonas Infections/microbiology ; Pseudomonas Infections/prevention & control ; Methacrylates |
| Abstract: | Competing Interests: Declaration of interest The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper of interest. Aim: Previous studies have demonstrated that contact lenses coated with the antimicrobial cationic peptide Mel4, a derivative of melimine, can reduce the occurrence of keratitis. However, the antimicrobial activity of Mel4 weakened over time due to its susceptibility to proteolytic degradation. Oligo-N-substituted glycine peptoids such as TM5 and TM18 possess antimicrobial properties and are resistant to proteolytic breakdown. This study focused on exploring methods for covalently attaching these peptoids to contact lenses to enhance their durability and performance in vitro. Methods: The peptoids TM5 and TM18 were covalently attached to etafilcon lenses via carbodiimide chemistry (EDC/NHS), oxazoline plasma, and plasma ion immersion implantation (PIII). The lenses were analysed using X-ray photoelectron spectroscopy (XPS), surface charge, and hydrophobicity. Inhibition of adhesion of multidrug-resistant Pseudomonas aeruginosa and cytotoxicity on corneal epithelial cells were evaluated. The impact of moist heat sterilization on activity was also assessed. Results: XPS confirmed peptoid binding to lenses. Peptoid coatings slightly increased contact angles (≤23°) without affecting overall charge. Peptoids, bound via carbodiimide, inhibited P. aeruginosa adhesion by over 5 log10 CFU per lens, outperforming melimine, which required six times the concentration for a 3 log10 reduction. Peptoids attached via oxazoline or PIII reduced adhesion by > 5 log10 CFU. All covalent methods significantly reduced bacterial adhesion compared to untreated lenses (P < 0.0001). Peptoid-bound lenses were non-toxic to corneal epithelial cells. Sterilization did not affect carbodiimide-treated lenses but reduced the activity of oxazoline and PIII surfaces by 1-2 log10 CFU. Conclusion: Peptoids TM5 and TM18 effectively reduced P. aeruginosa adhesion on lenses, with carbodiimide-bound surfaces retaining activity post-sterilization, showing promise for the development of antimicrobial contact lenses. (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| Contributed Indexing: | Keywords: Antimicrobial peptoids; Contact lens; Covalent attachment; Microbial keratitis; P. aeruginosa |
| Substance Nomenclature: | 0 (Peptoids) 0 (etafilcon) 0 (Methacrylates) |
| Entry Date(s): | Date Created: 20241121 Date Completed: 20241207 Latest Revision: 20241207 |
| Update Code: | 20250114 |
| DOI: | 10.1016/j.exer.2024.110149 |
| PMID: | 39571778 |
| Datenbank: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | Competing Interests: Declaration of interest The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper of interest.<br />Aim: Previous studies have demonstrated that contact lenses coated with the antimicrobial cationic peptide Mel4, a derivative of melimine, can reduce the occurrence of keratitis. However, the antimicrobial activity of Mel4 weakened over time due to its susceptibility to proteolytic degradation. Oligo-N-substituted glycine peptoids such as TM5 and TM18 possess antimicrobial properties and are resistant to proteolytic breakdown. This study focused on exploring methods for covalently attaching these peptoids to contact lenses to enhance their durability and performance in vitro.<br />Methods: The peptoids TM5 and TM18 were covalently attached to etafilcon lenses via carbodiimide chemistry (EDC/NHS), oxazoline plasma, and plasma ion immersion implantation (PIII). The lenses were analysed using X-ray photoelectron spectroscopy (XPS), surface charge, and hydrophobicity. Inhibition of adhesion of multidrug-resistant Pseudomonas aeruginosa and cytotoxicity on corneal epithelial cells were evaluated. The impact of moist heat sterilization on activity was also assessed.<br />Results: XPS confirmed peptoid binding to lenses. Peptoid coatings slightly increased contact angles (≤23°) without affecting overall charge. Peptoids, bound via carbodiimide, inhibited P. aeruginosa adhesion by over 5 log10 CFU per lens, outperforming melimine, which required six times the concentration for a 3 log10 reduction. Peptoids attached via oxazoline or PIII reduced adhesion by > 5 log10 CFU. All covalent methods significantly reduced bacterial adhesion compared to untreated lenses (P < 0.0001). Peptoid-bound lenses were non-toxic to corneal epithelial cells. Sterilization did not affect carbodiimide-treated lenses but reduced the activity of oxazoline and PIII surfaces by 1-2 log10 CFU.<br />Conclusion: Peptoids TM5 and TM18 effectively reduced P. aeruginosa adhesion on lenses, with carbodiimide-bound surfaces retaining activity post-sterilization, showing promise for the development of antimicrobial contact lenses.<br /> (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
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| ISSN: | 1096-0007 |
| DOI: | 10.1016/j.exer.2024.110149 |