Simultaneous suppression of lignin, tricin and wall-bound phenolic biosynthesis via the expression of monolignol 4-O-methyltransferases in rice.
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| Titel: | Simultaneous suppression of lignin, tricin and wall-bound phenolic biosynthesis via the expression of monolignol 4-O-methyltransferases in rice. |
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| Autoren: | Dwivedi N; Biology Department, Brookhaven Nation Laboratory, Upton, New York, USA.; Feedstocks Division, Joint BioEnergy Institute, Emeryville, CA, USA., Yamamoto S; Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto, Japan., Zhao Y; Biology Department, Brookhaven Nation Laboratory, Upton, New York, USA., Hou G; Dewel Microscopy Facility, Appalachian State University, Boone, North Carolina, USA., Bowling F; Biology Department, Brookhaven Nation Laboratory, Upton, New York, USA., Tobimatsu Y; Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto, Japan., Liu CJ; Biology Department, Brookhaven Nation Laboratory, Upton, New York, USA.; Feedstocks Division, Joint BioEnergy Institute, Emeryville, CA, USA. |
| Quelle: | Plant biotechnology journal [Plant Biotechnol J] 2024 Feb; Vol. 22 (2), pp. 330-346. Date of Electronic Publication: 2023 Oct 05. |
| Publikationsart: | Journal Article |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists Country of Publication: England NLM ID: 101201889 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1467-7652 (Electronic) Linking ISSN: 14677644 NLM ISO Abbreviation: Plant Biotechnol J Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2014- : Oxford Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists Original Publication: [Oxford] : Blackwell Pub., c2003- |
| MeSH-Schlagworte: | Methyltransferases*/genetics , Methyltransferases*/metabolism , Oryza*/genetics , Oryza*/metabolism, Lignin/metabolism ; Flavonoids/metabolism ; Cell Wall/metabolism |
| Abstract: | Grass lignocelluloses feature complex compositions and structures. In addition to the presence of conventional lignin units from monolignols, acylated monolignols and flavonoid tricin also incorporate into lignin polymer; moreover, hydroxycinnamates, particularly ferulate, cross-link arabinoxylan chains with each other and/or with lignin polymers. These structural complexities make grass lignocellulosics difficult to optimize for effective agro-industrial applications. In the present study, we assess the applications of two engineered monolignol 4-O-methyltransferases (MOMTs) in modifying rice lignocellulosic properties. Two MOMTs confer regiospecific para-methylation of monolignols but with different catalytic preferences. The expression of MOMTs in rice resulted in differential but drastic suppression of lignin deposition, showing more than 50% decrease in guaiacyl lignin and up to an 90% reduction in syringyl lignin in transgenic lines. Moreover, the levels of arabinoxylan-bound ferulate were reduced by up to 50%, and the levels of tricin in lignin fraction were also substantially reduced. Concomitantly, up to 11 μmol/g of the methanol-extractable 4-O-methylated ferulic acid and 5-7 μmol/g 4-O-methylated sinapic acid were accumulated in MOMT transgenic lines. Both MOMTs in vitro displayed discernible substrate promiscuity towards a range of phenolics in addition to the dominant substrate monolignols, which partially explains their broad effects on grass phenolic biosynthesis. The cell wall structural and compositional changes resulted in up to 30% increase in saccharification yield of the de-starched rice straw biomass after diluted acid-pretreatment. These results demonstrate an effective strategy to tailor complex grass cell walls to generate improved cellulosic feedstocks for the fermentable sugar-based production of biofuel and bio-chemicals. (© 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.) |
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| Grant Information: | JP20H03044 Japan Society for the Promotion of Science; JP 22J13457 Japan Society for the Promotion of Science; DE-AC02-05CH11231 U.S. Department of Energy; DE-C0018420 U.S. Department of Energy; DE-SC0012704 U.S. Department of Energy |
| Contributed Indexing: | Keywords: 4-O-methylated ferulic acid; Monolignol 4-O-methyltransferase; lignin; saccharification; tricin; wall-bound phenolics |
| Substance Nomenclature: | EC 2.1.1.- (Methyltransferases) D51JZL38TQ (tricin) 9005-53-2 (Lignin) 0 (Flavonoids) |
| Entry Date(s): | Date Created: 20231005 Date Completed: 20240131 Latest Revision: 20241023 |
| Update Code: | 20250114 |
| PubMed Central ID: | PMC10826995 |
| DOI: | 10.1111/pbi.14186 |
| PMID: | 37795899 |
| Datenbank: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | Grass lignocelluloses feature complex compositions and structures. In addition to the presence of conventional lignin units from monolignols, acylated monolignols and flavonoid tricin also incorporate into lignin polymer; moreover, hydroxycinnamates, particularly ferulate, cross-link arabinoxylan chains with each other and/or with lignin polymers. These structural complexities make grass lignocellulosics difficult to optimize for effective agro-industrial applications. In the present study, we assess the applications of two engineered monolignol 4-O-methyltransferases (MOMTs) in modifying rice lignocellulosic properties. Two MOMTs confer regiospecific para-methylation of monolignols but with different catalytic preferences. The expression of MOMTs in rice resulted in differential but drastic suppression of lignin deposition, showing more than 50% decrease in guaiacyl lignin and up to an 90% reduction in syringyl lignin in transgenic lines. Moreover, the levels of arabinoxylan-bound ferulate were reduced by up to 50%, and the levels of tricin in lignin fraction were also substantially reduced. Concomitantly, up to 11 μmol/g of the methanol-extractable 4-O-methylated ferulic acid and 5-7 μmol/g 4-O-methylated sinapic acid were accumulated in MOMT transgenic lines. Both MOMTs in vitro displayed discernible substrate promiscuity towards a range of phenolics in addition to the dominant substrate monolignols, which partially explains their broad effects on grass phenolic biosynthesis. The cell wall structural and compositional changes resulted in up to 30% increase in saccharification yield of the de-starched rice straw biomass after diluted acid-pretreatment. These results demonstrate an effective strategy to tailor complex grass cell walls to generate improved cellulosic feedstocks for the fermentable sugar-based production of biofuel and bio-chemicals.<br /> (© 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.) |
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| ISSN: | 1467-7652 |
| DOI: | 10.1111/pbi.14186 |