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Optical glucose sensor for microfluidic cell culture systems.

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Bibliographic Details
Title: Optical glucose sensor for microfluidic cell culture systems.
Authors: Fuchs S; Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/II, 8010, Graz, Austria., Rieger V; Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/II, 8010, Graz, Austria., Tjell AØ; Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/II, 8010, Graz, Austria., Spitz S; Institute of Applied Synthetic Chemistry and Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060, Vienna, Austria., Brandauer K; Institute of Applied Synthetic Chemistry and Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060, Vienna, Austria., Schaller-Ammann R; HEALTH - Institute for Biomedical Research and Technologies, Joanneum Research Forschungsgesellschaft m.b.H, Neue Stiftingtalstraße 2, 8010, Graz, Austria., Feiel J; HEALTH - Institute for Biomedical Research and Technologies, Joanneum Research Forschungsgesellschaft m.b.H, Neue Stiftingtalstraße 2, 8010, Graz, Austria., Ertl P; Institute of Applied Synthetic Chemistry and Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060, Vienna, Austria., Klimant I; Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/II, 8010, Graz, Austria., Mayr T; Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/II, 8010, Graz, Austria. Electronic address: torsten.mayr@tugraz.at.
Source: Biosensors & bioelectronics [Biosens Bioelectron] 2023 Oct 01; Vol. 237, pp. 115491. Date of Electronic Publication: 2023 Jun 25.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE
Imprint Name(s): Publication: Oxford : Elsevier Advanced Technology
Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989-
MeSH Terms: Microfluidic Analytical Techniques*/methods , Biosensing Techniques*/methods, Humans ; Microfluidics ; Blood Glucose Self-Monitoring ; Blood Glucose ; Cell Culture Techniques/methods ; Glucose/metabolism ; Oxygen/metabolism
Abstract: Glucose is the primary energy source of human cells. Therefore, monitoring glucose inside microphysiological systems (MPS) provides valuable information on the viability and metabolic state of the cultured cells. However, continuous glucose monitoring inside MPS is challenging due to a lack of suitable miniaturized sensors. Here we present an enzymatic, optical glucose sensor element for measurement inside microfluidic systems. The miniaturized glucose sensor (Ø 1 mm) is fabricated together with a reference oxygen sensor onto biocompatible, pressure-sensitive adhesive tape for easy integration inside microfluidic systems. Furthermore, the proposed microfluidic system can be used as plug and play sensor system with existing MPS. It was characterized under cell culture conditions (37 °C and pH 7.4) for five days, exhibiting minor drift (3% day -1 ). The influence of further cell culture parameters like oxygen concentration, pH, flow rate, and sterilization methods was investigated. The plug-and-play system was used for at-line measurements of glucose levels in (static) cell culture and achieved good agreement with a commercially available glucose sensor. In conclusion, we developed an optical glucose sensor element that can be easily integrated in microfluidic systems and is able to perform stable glucose measurements under cell culture conditions.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Torsten Mayr reports a relationship with PyroScience GmbH that includes: board membership, employment, and equity or stocks. Ingo Klimant reports a relationship with PyroScience GmbH that includes: board membership and equity or stocks.
Contributed Indexing: Keywords: Flow through cell; Glucose; Microfluidic; Microphysiological systems; Optical sensor; Organ-on-Chip
Substance Nomenclature: 0 (Blood Glucose)
IY9XDZ35W2 (Glucose)
S88TT14065 (Oxygen)
Entry Date(s): Date Created: 20230706 Date Completed: 20230814 Latest Revision: 20230814
Update Code: 20250114
DOI: 10.1016/j.bios.2023.115491
PMID: 37413826
Database: MEDLINE
Description
Abstract:Glucose is the primary energy source of human cells. Therefore, monitoring glucose inside microphysiological systems (MPS) provides valuable information on the viability and metabolic state of the cultured cells. However, continuous glucose monitoring inside MPS is challenging due to a lack of suitable miniaturized sensors. Here we present an enzymatic, optical glucose sensor element for measurement inside microfluidic systems. The miniaturized glucose sensor (Ø 1 mm) is fabricated together with a reference oxygen sensor onto biocompatible, pressure-sensitive adhesive tape for easy integration inside microfluidic systems. Furthermore, the proposed microfluidic system can be used as plug and play sensor system with existing MPS. It was characterized under cell culture conditions (37 °C and pH 7.4) for five days, exhibiting minor drift (3% day <sup>-1</sup> ). The influence of further cell culture parameters like oxygen concentration, pH, flow rate, and sterilization methods was investigated. The plug-and-play system was used for at-line measurements of glucose levels in (static) cell culture and achieved good agreement with a commercially available glucose sensor. In conclusion, we developed an optical glucose sensor element that can be easily integrated in microfluidic systems and is able to perform stable glucose measurements under cell culture conditions.<br /> (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
ISSN:1873-4235
DOI:10.1016/j.bios.2023.115491