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Binding versus catalysis in de novo enzyme design.

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Bibliographic Details
Title: Binding versus catalysis in de novo enzyme design.
Authors: Sanchez-Ruiz, Jose M.1 (AUTHOR) sanchezr@ugr.es
Source: Biocatalysis & Biotransformation. Oct2025, p1-4. 4p. 1 Illustration.
Subject Terms: *CATALYSIS, *ENZYME kinetics, *ENZYME biotechnology, *CATALYSTS, *SYNTHETIC enzymes, *BINDING sites, *APPLIED sciences
Abstract: AbstractThe catalytic parameters describing enzyme catalysis are discussed in the context of the design of de novo enzymes. It is shown that high values of the catalytic efficiency may in some cases reflect tight substrate binding and lead to an immediate levelling-off of rates to reach a low plateau, an undesirable scenario for many industrial applications. It is argued that increasing the catalytic rate constant should be a main target in de novo enzyme engineering. [ABSTRACT FROM AUTHOR]
Database: Academic Search Index
Description
Abstract:AbstractThe catalytic parameters describing enzyme catalysis are discussed in the context of the design of <italic>de novo</italic> enzymes. It is shown that high values of the catalytic efficiency may in some cases reflect tight substrate binding and lead to an immediate levelling-off of rates to reach a low plateau, an undesirable scenario for many industrial applications. It is argued that increasing the catalytic rate constant should be a main target in <italic>de novo</italic> enzyme engineering. [ABSTRACT FROM AUTHOR]
ISSN:10242422
DOI:10.1080/10242422.2025.2577182