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Optimization and characterization of acute radiation-induced esophagitis in mice.

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Název: Optimization and characterization of acute radiation-induced esophagitis in mice.
Autoři: Feng, Jianhua1,2 (AUTHOR), Liu, Xiangjun1,2 (AUTHOR), Xu, Lu2 (AUTHOR), Wang, Ke2 (AUTHOR), He, Simin1,2 (AUTHOR), Wang, Xi2 (AUTHOR), Huang, Yujun2 (AUTHOR), Wang, Shubin2 (AUTHOR), Lei, Xudan2 (AUTHOR), Huang, Lingxiao2 (AUTHOR), Xu, Zhenni2 (AUTHOR), Lang, Jinyi1 (AUTHOR) langjinyi@scszlyy.org.cn, Liu, Dengqun2 (AUTHOR) dengqunliu@uestc.edu.cn, Yin, Jun1,3 (AUTHOR) gzyinjun@163.com
Zdroj: Radiation Oncology. 9/30/2025, Vol. 20 Issue 1, p1-15. 15p.
Témata: *RADIATION doses, *RADIATION damage, *INFLAMMATION, *APOPTOSIS, *TISSUE wounds, *RADIOTHERAPY, *LABORATORY mice, *HISTOPATHOLOGY
Abstrakt: Purpose: To optimize an animal model of acute radiation-induced esophagitis (RIE) in C57BL/6 mice and characterize the histopathological features of RIE at different stages. Materials and methods: C57BL/6 mice were subjected to single thoracic X-ray irradiation at doses ranging from 5 to 30 Gy. Changes in body weight, daily food and water intake, and survival were monitored and compared within 2 weeks after radiation exposure. Epithelial damage to the esophagus, apoptosis, and inflammation at different times after irradiation were examined to characterize the pathological process of RIE. Results: The incidence of acute RIE was strongly correlated with increasing radiation dose across all the experimental groups. No deaths were observed in mice that received 5 or 10 Gy of irradiation, whereas complete mortality was observed within 15 days after exposure to 30 Gy of irradiation. The mice in 20 Gy irradiation group had a low mortality rate. The peak of esophageal tissue damage occurred at Day 7 and was healed by Day 14 after exposure to 20 Gy of thoracic irradiation. The pathology of RIE was induced by radiation-induced DNA damage, apoptosis, reactive oxygen species (ROS), and mitochondrial impairment. Conclusions: In this study, we found that a single 20 Gy thoracic irradiation was the optimal dose to establish acute RIE in C57BL/6 mice. Acute esophageal injury peaked on Day 7 after radiation, and the process of regeneration and repair was complete within 14 days. This work may be a useful reference for experimental research concerning RIE. [ABSTRACT FROM AUTHOR]
Databáze: Academic Search Index
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Abstrakt:Purpose: To optimize an animal model of acute radiation-induced esophagitis (RIE) in C57BL/6 mice and characterize the histopathological features of RIE at different stages. Materials and methods: C57BL/6 mice were subjected to single thoracic X-ray irradiation at doses ranging from 5 to 30 Gy. Changes in body weight, daily food and water intake, and survival were monitored and compared within 2 weeks after radiation exposure. Epithelial damage to the esophagus, apoptosis, and inflammation at different times after irradiation were examined to characterize the pathological process of RIE. Results: The incidence of acute RIE was strongly correlated with increasing radiation dose across all the experimental groups. No deaths were observed in mice that received 5 or 10 Gy of irradiation, whereas complete mortality was observed within 15 days after exposure to 30 Gy of irradiation. The mice in 20 Gy irradiation group had a low mortality rate. The peak of esophageal tissue damage occurred at Day 7 and was healed by Day 14 after exposure to 20 Gy of thoracic irradiation. The pathology of RIE was induced by radiation-induced DNA damage, apoptosis, reactive oxygen species (ROS), and mitochondrial impairment. Conclusions: In this study, we found that a single 20 Gy thoracic irradiation was the optimal dose to establish acute RIE in C57BL/6 mice. Acute esophageal injury peaked on Day 7 after radiation, and the process of regeneration and repair was complete within 14 days. This work may be a useful reference for experimental research concerning RIE. [ABSTRACT FROM AUTHOR]
ISSN:1748717X
DOI:10.1186/s13014-025-02720-w