Effect of cold pre-treatment of panicle on induction and regeneration of callus from anther culture of seven rice genotypes.
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| Název: | Effect of cold pre-treatment of panicle on induction and regeneration of callus from anther culture of seven rice genotypes. |
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| Autoři: | Iriani, Yoanita Fadlilah1,2 yoanita204@gmail.com, Prayantini, Dian Catur2, Mastuti, Retno1, Harijati, Nunung1 |
| Zdroj: | Journal of Biological Researches. Aug2025, Vol. 31 Issue 2, p103-107. 5p. |
| Témata: | *RICE, *PLANT tissue culture, *PLANT breeding, *HAPLOIDY, *IN vitro studies, *LOW temperature engineering, *CALLUS |
| Abstrakt: | Anther culture is an in vitro culture technique to produce double haploid plants with homozygous traits, which can be used to make new varieties of plants more quickly. The success of anther culture was influenced by several factors, including plant genotype, culture media composition, culture environment conditions (temperature and light), and pretreatment of anther before culturing. Cold temperature pretreatment was an important factor to support the success of anther culture. The purpose of this study was to determine the effect of cold pretreatment on panicle induction and callus regeneration from anther culture of seven Indica rice genotypes (Oryza sativa L.). Panicles used as explants were harvested, then pretreated with cold temperatures of 4°C and 8°C for 8 days. The anther was cultured on callus induction media consisting of 3,21 g/L B5 Gamborg, 50 g/L maltose, 2 g/L Gelrite, 2,5 mg/L NAA, 1 mg/L Kinetin, with 2 mg/L 2,4-D. The formation of callus is observed to occur at a period of four to six weeks following the culture process. The regeneration of callus is facilitated by a medium specifically formulated for this purpose, comprising MS medium along with additional components, 30 g/L sucrose, 2 g/L Gelrite, 1 mg/L BAP, 1 mg/L Kinetin, 1 mg/L NAA, pH 5,8. Two weeks after the callus was regenerated, the percentage of green callus and browning callus formed was calculated. The 8°C cold pretreatment produced the highest green callus, while the highest browning callus was obtained in the 4°C cold pretreatment. The total callus formed was positively correlated with the total green regenerants produced. Cold pretreatment at 8°C for 8 days can increase the responsiveness of anther culture by providing the best results for induction and regeneration in the seven plant genotypes used in this study. [ABSTRACT FROM AUTHOR] |
| Databáze: | Academic Search Index |
Nájsť tento článok vo Web of Science | Abstrakt: | Anther culture is an in vitro culture technique to produce double haploid plants with homozygous traits, which can be used to make new varieties of plants more quickly. The success of anther culture was influenced by several factors, including plant genotype, culture media composition, culture environment conditions (temperature and light), and pretreatment of anther before culturing. Cold temperature pretreatment was an important factor to support the success of anther culture. The purpose of this study was to determine the effect of cold pretreatment on panicle induction and callus regeneration from anther culture of seven Indica rice genotypes (Oryza sativa L.). Panicles used as explants were harvested, then pretreated with cold temperatures of 4°C and 8°C for 8 days. The anther was cultured on callus induction media consisting of 3,21 g/L B5 Gamborg, 50 g/L maltose, 2 g/L Gelrite, 2,5 mg/L NAA, 1 mg/L Kinetin, with 2 mg/L 2,4-D. The formation of callus is observed to occur at a period of four to six weeks following the culture process. The regeneration of callus is facilitated by a medium specifically formulated for this purpose, comprising MS medium along with additional components, 30 g/L sucrose, 2 g/L Gelrite, 1 mg/L BAP, 1 mg/L Kinetin, 1 mg/L NAA, pH 5,8. Two weeks after the callus was regenerated, the percentage of green callus and browning callus formed was calculated. The 8°C cold pretreatment produced the highest green callus, while the highest browning callus was obtained in the 4°C cold pretreatment. The total callus formed was positively correlated with the total green regenerants produced. Cold pretreatment at 8°C for 8 days can increase the responsiveness of anther culture by providing the best results for induction and regeneration in the seven plant genotypes used in this study. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 08526834 |
| DOI: | 10.23869/bphjbr.31.2.20255 |