Multiplication of Actinidia chinensis var. deliciosa in three in vitro culture systems.
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| Názov: | Multiplication of Actinidia chinensis var. deliciosa in three in vitro culture systems. |
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| Autori: | López-Páez, Fátima Karina1 (AUTHOR), Galindo-Tovar, María Elena1 (AUTHOR), García-Martínez, Miguel Ángel1 (AUTHOR), Serna-Lagunes, Ricardo1 (AUTHOR), Solano-Rodríguez, Luis Alberto2 (AUTHOR), Pastelín-Solano, Miriam Cristina2 (AUTHOR), Castañeda-Castro, Odón2 (AUTHOR), Cruz-Castillo, Juan Guillermo3 (AUTHOR) jcruzc@chapingo.mx |
| Zdroj: | New Zealand Journal of Crop & Horticultural Science. Dec2025, Vol. 53 Issue 5, p2786-2796. 11p. |
| Predmety: | *KIWIFRUIT, *IN vitro studies, *TROPICAL conditions, *CROP science, *CHLOROPHYLL, *PLANT tissue culture, *PLANT propagation |
| Geografický termín: | MEXICO |
| Abstrakt: | In Mexico, kiwifruit has adapted to high tropical climates, but plant numbers remain limited for its commercial cultivation. In vitro cultivation offers a solution for its propagation and commercial introduction. This study compared semisolid medium, partial immersion, and temporary bioreactor (BIT®) in vitro systems for plant multiplication and field introduction. For the BIT® system, five immersion times (2, 3, 5, 8, and 11 min) were tested for multiplication. After 60 days, the number of shoots, roots, and leaves, along with root and shoot size, were analysed using normality tests, parametric, non-parametric and principal component analysis. The best immersion time for BIT® was eight minutes, resulting in the highest shoot production. When the three systems were compared, partial immersion and semisolid medium systems supported chlorophyll content, root development, and acclimatisation, enhancing plant survival. The BIT® system proved more efficiency for leaf and shoot multiplication due to periodic oxygenation, but it had the lowest acclimatisation percentage. However, with optimal multiplication and a phytohormonal treatment before acclimatisation, plant production for commercial introduction could be achieved. Field introduction was successful in Ixhuatlán del café, Veracruz, México, demonstrating the potential of in vitro techniques for kiwifruit plants production and their cultivation. [ABSTRACT FROM AUTHOR] |
| Databáza: | Academic Search Index |
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Nájsť tento článok vo Web of Science | Abstrakt: | In Mexico, kiwifruit has adapted to high tropical climates, but plant numbers remain limited for its commercial cultivation. In vitro cultivation offers a solution for its propagation and commercial introduction. This study compared semisolid medium, partial immersion, and temporary bioreactor (BIT®) in vitro systems for plant multiplication and field introduction. For the BIT® system, five immersion times (2, 3, 5, 8, and 11 min) were tested for multiplication. After 60 days, the number of shoots, roots, and leaves, along with root and shoot size, were analysed using normality tests, parametric, non-parametric and principal component analysis. The best immersion time for BIT® was eight minutes, resulting in the highest shoot production. When the three systems were compared, partial immersion and semisolid medium systems supported chlorophyll content, root development, and acclimatisation, enhancing plant survival. The BIT® system proved more efficiency for leaf and shoot multiplication due to periodic oxygenation, but it had the lowest acclimatisation percentage. However, with optimal multiplication and a phytohormonal treatment before acclimatisation, plant production for commercial introduction could be achieved. Field introduction was successful in Ixhuatlán del café, Veracruz, México, demonstrating the potential of in vitro techniques for kiwifruit plants production and their cultivation. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 01140671 |
| DOI: | 10.1080/01140671.2025.2507865 |