Single-cell spatial mapping reveals alteration of tissue microenvironment during early colorectal cancer

Familial adenomatous polyposis (FAP) is a rare, hereditary syndrome that raises the risk of developing colorectal cancer (CRC). This disease model is well suited for studying the early stages of malignant transformation. Our spatial CODEX experiments reveal that, in contrast to normal mucosa, FAP mu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:bioRxiv
Hauptverfasser: Guha, Tuhin K, Esplin, Edward D, Horning, Aaron M, Chiu, Roxanne, Paul, Kristina, Weimer, Annika K, Becker, Winston R, Laquindanum, Rozelle, Mills, Meredith A, Esplin, D Glen, Shen, Jeanne, Monte, Emma, White, Shannon, Karathanos, Thomas V, Cotter, Daniel, Bi, Joanna, Ladabaum, Uri, Longacre, Teri A, Curtis, Christina, Ford, James M, Greenleaf, William J, Snyder, Michael P
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 27.08.2025
Schlagworte:
Familial adenomatous polyposis (FAP)
pre-cancer microenvironment
colorectal cancer
cell type composition
tumor microenvironment
Co-detection by Indexing (CODEX)
neighborhood analysis
ISSN:2692-8205
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Familial adenomatous polyposis (FAP) is a rare, hereditary syndrome that raises the risk of developing colorectal cancer (CRC). This disease model is well suited for studying the early stages of malignant transformation. Our spatial CODEX experiments reveal that, in contrast to normal mucosa, FAP mucosa, pre-cancer polyps and colorectal cancers exhibit substantial alterations in the cell type composition and tissue microenvironment. These early alterations include: an increase in the population of cancer-associated fibroblasts (CAFs), and the inhibition of tumor infiltrated lymphocytes and cell-adhesion protein by CAFs, the transformation of memory T cells into regulatory T cells, nuclear translocation of beta-catenin from the cell membrane, a decrease in the M1:M2 macrophage ratio, a notable increase in angiogenesis events. Our studies define the early stem cell, stromal, and immune steps of colorectal cancer and may benefit early detection, and therapeutic intervention.
ISSN:2692-8205
DOI:10.1101/2024.11.20.622725