Exploratory study on detection of drug resistance of Mycobacterium tuberculosis in sputum specimens by next-generation sequencing
To compare the diagnostic performance of next-generation sequencing (NGS) detection methods in sputum samples and strains, in order to explore the feasibility of the NGS method to detect drug resistance in sputum specimens. In this retrospective study, the sputum specimens and corresponding clinical...
Gespeichert in:
| Veröffentlicht in: | Zhonghua jiehe he huxi zazhi Jg. 45; H. 6; S. 552 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Journal Article |
| Sprache: | Chinesisch |
| Veröffentlicht: |
China
12.06.2022
|
| Schlagworte: | |
| ISSN: | 1001-0939 |
| Online-Zugang: | Weitere Angaben |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | To compare the diagnostic performance of next-generation sequencing (NGS) detection methods in sputum samples and
strains, in order to explore the feasibility of the NGS method to detect drug resistance in sputum specimens.
In this retrospective study, the sputum specimens and corresponding clinical isolates of 50 pulmonary tuberculosis patients admitted to Beijing Chest Hospital from January 2017 to December 2017 were collected. The gene mutations of
,
,
,
,
,
,
,
,
and
in sputum specimens and corresponding clinical isolates were detected by NGS method. The phenotypic drug susceptibility test (DST) of the strains was carried out by the proportion method. Using DST results as a reference, the sensitivity, specificity, positive predictive value and negative predictive value of the NGS method for clinical strains and sputum specimens, as well as the consistency statistic (
) with phenotype DST were calculated respectively. The Chi-square test was used to compare the accuracy of the NGS testing in sputum samples |
|---|---|
| AbstractList | Objective: To compare the diagnostic performance of next-generation sequencing (NGS) detection methods in sputum samples and Mycobacterium tuberculosis strains, in order to explore the feasibility of the NGS method to detect drug resistance in sputum specimens. Methods: In this retrospective study, the sputum specimens and corresponding clinical isolates of 50 pulmonary tuberculosis patients admitted to Beijing Chest Hospital from January 2017 to December 2017 were collected. The gene mutations of katG, inhA, rpoB, embA, embB, rpsL, rrs, gyrA, gyrB and tlyA in sputum specimens and corresponding clinical isolates were detected by NGS method. The phenotypic drug susceptibility test (DST) of the strains was carried out by the proportion method. Using DST results as a reference, the sensitivity, specificity, positive predictive value and negative predictive value of the NGS method for clinical strains and sputum specimens, as well as the consistency statistic (Kappa) with phenotype DST were calculated respectivel To compare the diagnostic performance of next-generation sequencing (NGS) detection methods in sputum samples and strains, in order to explore the feasibility of the NGS method to detect drug resistance in sputum specimens. In this retrospective study, the sputum specimens and corresponding clinical isolates of 50 pulmonary tuberculosis patients admitted to Beijing Chest Hospital from January 2017 to December 2017 were collected. The gene mutations of , , , , , , , , and in sputum specimens and corresponding clinical isolates were detected by NGS method. The phenotypic drug susceptibility test (DST) of the strains was carried out by the proportion method. Using DST results as a reference, the sensitivity, specificity, positive predictive value and negative predictive value of the NGS method for clinical strains and sputum specimens, as well as the consistency statistic ( ) with phenotype DST were calculated respectively. The Chi-square test was used to compare the accuracy of the NGS testing in sputum samples |
| Author | Guo, Y L Li, L Chu, P Lu, J Wu, T Y Liu, Y Y Pang, Y Shi, J |
| Author_xml | – sequence: 1 givenname: Y Y surname: Liu fullname: Liu, Y Y organization: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China – sequence: 2 givenname: J surname: Shi fullname: Shi, J organization: Department of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China – sequence: 3 givenname: P surname: Chu fullname: Chu, P organization: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China – sequence: 4 givenname: T Y surname: Wu fullname: Wu, T Y organization: Graduate School of Inner Mongolia Medical University, Hohhot 010059, China – sequence: 5 givenname: L surname: Li fullname: Li, L organization: Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China – sequence: 6 givenname: Y surname: Pang fullname: Pang, Y organization: Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China – sequence: 7 givenname: J surname: Lu fullname: Lu, J organization: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China – sequence: 8 givenname: Y L surname: Guo fullname: Guo, Y L organization: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35658379$$D View this record in MEDLINE/PubMed |
| BookMark | eNo1kEtPwzAQhH0ooqX0LyBfkLik2E4cx0dU8ZKKuPQeOc6mMkrs4IfUHPnnpKKcdnf0aWa0N2hhnQWE7inZ5qIkj3pQ26-ttpQyWoiMEUYpJUVGiBB8gVaUEJoRmcsl2oRgmvmkJOdUXqNlzkte5UKu0M_zaeydV9H5CYeY2gk7i1uIoKOZN9fh1qcj9hBMiMpqOEsfk3aN0hG8SQOOqQGvU-9mBBuLw5jiLIcRtBnABtxM2MIpZkewMEedfQN8J7Da2OMtuupUH2BzmWt0eHk-7N6y_efr--5pn428lJmemwvNgGpOKt1oyrhuBVOyEl3eMcVAKUVbSkTRyqYQeSW4IlBIVlYFlB1bo4c_29G7OTrEejBBQ98rCy6FmpUi51KUFZnRuwuamgHaevRmUH6q_7_GfgGD8na0 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.3760/cma.j.cn112147-20211104-00775 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| ExternalDocumentID | 35658379 |
| Genre | Journal Article |
| GrantInformation_xml | – fundername: National Natural Science Foundation of China grantid: 81401739 – fundername: Special Fund of The Pediatric Medical Coordinated Development Center of Beijing Municipal Administration of Hospitals grantid: XTCX201806 – fundername: Beijing Talent Training Funded Project grantid: 2018000021469G277 |
| GroupedDBID | --- -05 123 92F 92I ABJNI ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CGR CIEJG CUY CVF CW9 ECM EIF F5P NPM TCJ TGQ U5O 7X8 |
| ID | FETCH-LOGICAL-p569-c3517c2e1c508cbc125cd72a987f3f2a2eaaa1d1074d9b473875a0e492684e6f2 |
| IEDL.DBID | 7X8 |
| ISSN | 1001-0939 |
| IngestDate | Wed Oct 01 17:20:32 EDT 2025 Thu Apr 03 07:09:44 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Language | Chinese |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-p569-c3517c2e1c508cbc125cd72a987f3f2a2eaaa1d1074d9b473875a0e492684e6f2 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 35658379 |
| PQID | 2673597680 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_2673597680 pubmed_primary_35658379 |
| PublicationCentury | 2000 |
| PublicationDate | 2022-Jun-12 20220612 |
| PublicationDateYYYYMMDD | 2022-06-12 |
| PublicationDate_xml | – month: 06 year: 2022 text: 2022-Jun-12 day: 12 |
| PublicationDecade | 2020 |
| PublicationPlace | China |
| PublicationPlace_xml | – name: China |
| PublicationTitle | Zhonghua jiehe he huxi zazhi |
| PublicationTitleAlternate | Zhonghua Jie He He Hu Xi Za Zhi |
| PublicationYear | 2022 |
| SSID | ssib001103519 ssib051368298 ssj0042058 |
| Score | 2.3393128 |
| Snippet | To compare the diagnostic performance of next-generation sequencing (NGS) detection methods in sputum samples and
strains, in order to explore the feasibility... Objective: To compare the diagnostic performance of next-generation sequencing (NGS) detection methods in sputum samples and Mycobacterium tuberculosis... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 552 |
| SubjectTerms | Amikacin - pharmacology Antitubercular Agents - pharmacology Antitubercular Agents - therapeutic use Capreomycin - pharmacology Drug Resistance, Multiple, Bacterial - genetics High-Throughput Nucleotide Sequencing - methods Humans Isoniazid - pharmacology Kanamycin - pharmacology Levofloxacin - pharmacology Microbial Sensitivity Tests Mycobacterium tuberculosis Retrospective Studies Rifampin - pharmacology Sputum - microbiology Streptomycin - pharmacology Tuberculosis, Lymph Node Tuberculosis, Multidrug-Resistant - diagnosis |
| Title | Exploratory study on detection of drug resistance of Mycobacterium tuberculosis in sputum specimens by next-generation sequencing |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/35658379 https://www.proquest.com/docview/2673597680 |
| Volume | 45 |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV07T8MwELaAIsTCQ7xfMhKMKY2d2PGEEAIx0KoDQ7fKsR0egqQ0DVLZ-OfcOaGwICGxZLASS7lc7r7z3X1HyAm2Z0oW64BH2kKAIrJAW6TcT4XlcZiJxHgS11vZ6yWDgeo3B25lU1b5ZRO9obaFwTPyMyYkB_Arks756DXAqVGYXW1GaMyTFgcog1otBz-yQCHmyWbuOg65SJiaZRki1onrVjkMqRVXS-QUo1gpOmfmRbef2iYHOBIilwG4QtgtCjxl3O9o1Hul69X_vs8aWWnwKL2oFWidzL0_bJCPujLPJ-CpJ6ClRU6tm_i6rZwWGbXj6p5CqI7wE_QGl7pTA8bBkz9XL3RSpW5squcCbqGPOS1H1QSWsbMTBwqUNJ3SHMPue0987fdt6rrBm26Su-uru8uboJnVEIxioQIDcpaGudAA4DOpAdhkrGRaJTLjGdPMaa1Di9WfVqWR5BAm6Y5DtsIkciJjW2QhL3K3Q2iaIsEND22UiSgxQsfWWRcZlYZgLYzeJcdfAh3Cr4D5DZ27oiqH3yLdJdv1VxmOas6OIQfgCrG42vvD0_tkmWGTg59QdEBaGRgCd0gWzdvksRwfeR2Da6_f_QTiENrg |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Exploratory+study+on+detection+of+drug+resistance+of+Mycobacterium+tuberculosis+in+sputum+specimens+by+next-generation+sequencing&rft.jtitle=Zhonghua+jiehe+he+huxi+zazhi&rft.au=Liu%2C+Y+Y&rft.au=Shi%2C+J&rft.au=Chu%2C+P&rft.au=Wu%2C+T+Y&rft.date=2022-06-12&rft.issn=1001-0939&rft.volume=45&rft.issue=6&rft.spage=552&rft_id=info:doi/10.3760%2Fcma.j.cn112147-20211104-00775&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1001-0939&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1001-0939&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1001-0939&client=summon |