CELL CULTURES PURITY CONTROL BY METHODS OF CLINICAL DIAGNOSTICS
Cell cultures of higher organisms, especially cultures of human cells, are increasingly used in medical, pharmaceutical and scientific research. The main problem of cell cultures — non-lethal hidden contamination by mycoplasmas, viruses and outsider cell lines. As an available and reliable method fo...
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| Vydané v: | T͡S︡itologii͡a Ročník 59; číslo 2; s. 99 |
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| Hlavní autori: | , , , |
| Médium: | Journal Article |
| Jazyk: | English Russian |
| Vydavateľské údaje: |
Russia (Federation)
2017
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| ISSN: | 0041-3771 |
| On-line prístup: | Zistit podrobnosti o prístupe |
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| Shrnutí: | Cell cultures of higher organisms, especially cultures of human cells, are increasingly used in medical,
pharmaceutical and scientific research. The main problem of cell cultures — non-lethal hidden contamination
by mycoplasmas, viruses and outsider cell lines. As an available and reliable method for monitoring the purity
of the cell cultures, we offer to use PCR kits designed and officially used in clinical diagnostics. We have tested
50 human cell lines using commercial diagnostic systems for detection of papilloma viruses, herpes viruses,
adenoviruses, Mycoplasma hominis and total bacterial mass. Contamination in tested cell lines was not found.
In the case of cell lines that contain integrated parts of viral genomes, the presence of the respective DNA sequences
was confirmed. The proposed diagnostic systems can be effectively used to control the purity of cell lines,
for qualitative detection of possible contamination, as well as for quantitative evaluations with calculation
of viral load like it is practiced in clinical diagnostics. |
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| Bibliografia: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0041-3771 |