Long‐term expanding human airway organoids for disease modeling
Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway o...
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| Published in: | The EMBO journal Vol. 38; no. 4 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
London
Nature Publishing Group UK
15.02.2019
Springer Nature B.V John Wiley and Sons Inc |
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| ISSN: | 0261-4189, 1460-2075, 1460-2075 |
| Online Access: | Get full text |
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| Abstract | Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non‐structural viral NS2 protein, and preferentially recruits neutrophils upon co‐culturing. We conclude that human airway organoids represent versatile models for the
in vitro
study of hereditary, malignant, and infectious pulmonary disease.
Synopsis
To date, persistent
in vitro
culture of adult human lung epithelium remains elusive. In this methods resource article, culture conditions to maintain three‐dimensional pulmonary tissue long‐term are reported and applied to recapitulate related diseases.
Culture conditions for long‐term expansion of healthy, hereditary disease and malignant human airway epithelial organoids.
Airway organoids are amenable for medium‐throughput drug screening.
Airway organoids readily allow modeling of viral infection.
Graphical Abstract
Three‐dimensional human pulmonary tissue culture allows for investigation of hereditary diseases. |
|---|---|
| AbstractList | Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non‐structural viral NS2 protein, and preferentially recruits neutrophils upon co‐culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease. Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non‐structural viral NS2 protein, and preferentially recruits neutrophils upon co‐culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease. Synopsis To date, persistent in vitro culture of adult human lung epithelium remains elusive. In this methods resource article, culture conditions to maintain three‐dimensional pulmonary tissue long‐term are reported and applied to recapitulate related diseases. Culture conditions for long‐term expansion of healthy, hereditary disease and malignant human airway epithelial organoids. Airway organoids are amenable for medium‐throughput drug screening. Airway organoids readily allow modeling of viral infection. Graphical Abstract Three‐dimensional human pulmonary tissue culture allows for investigation of hereditary diseases. Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non‐structural viral NS2 protein, and preferentially recruits neutrophils upon co‐culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease. Synopsis To date, persistent in vitro culture of adult human lung epithelium remains elusive. In this methods resource article, culture conditions to maintain three‐dimensional pulmonary tissue long‐term are reported and applied to recapitulate related diseases. Culture conditions for long‐term expansion of healthy, hereditary disease and malignant human airway epithelial organoids. Airway organoids are amenable for medium‐throughput drug screening. Airway organoids readily allow modelng of viral infection. Three‐dimensional human pulmonary tissue culture allows for investigation of hereditary diseases. Organoids are self-organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long-term-expanding human airway organoids from broncho-alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi-ciliated cells, mucus-producing secretory cells, and CC10-secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non-structural viral NS2 protein, and preferentially recruits neutrophils upon co-culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease.Organoids are self-organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long-term-expanding human airway organoids from broncho-alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi-ciliated cells, mucus-producing secretory cells, and CC10-secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non-structural viral NS2 protein, and preferentially recruits neutrophils upon co-culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease. Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non‐structural viral NS2 protein, and preferentially recruits neutrophils upon co‐culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease. Organoids are self-organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long-term-expanding human airway organoids from broncho-alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi-ciliated cells, mucus-producing secretory cells, and CC10-secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non-structural viral NS2 protein, and preferentially recruits neutrophils upon co-culturing. We conclude that human airway organoids represent versatile models for the study of hereditary, malignant, and infectious pulmonary disease. |
| Author | Heo, Inha Zomer‐van Ommen, Domenique D Voest, Emile E Coenjaerts, Frank E Clevers, Hans Weeber, Fleur Proost, Natalie Amatngalim, Gimano D Offerhaus, G Johan Sachs, Norman van der Ent, Cornelis K van Oudenaarden, Alexander Begthel, Harry Olimpio, Eduardo P Iakobachvili, Nino Teeven, Luc Ramos, Emilio Tans, Sander J Vries, Robert G van Oosterhout, Matthijs FM van der Linden, Maarten Böttinger, Lena Korving, Jeroen Bont, Louis J Peters, Peter J van Moorsel, Coline HM van Hoeck, Arne Viveen, Marco C Meyaard, Linde Dekkers, Johanna F van de Ven, Marieke Dijkstra, Krijn K Boj, Sylvia F Papaspyropoulos, Angelos Rios, Anne C Lyubimova, Anna Smit, Egbert F de Ligt, Joep Derakhshan, Sepideh Kumawat, Kuldeep Jaksani, Sridevi Wiener, Dominique J Jonkers, Jos Beekman, Jeffrey M Cuppen, Edwin Huelsz‐Prince, Guizela Klay, Dymph van Zon, Jeroen S |
| AuthorAffiliation | 2 Wilhelmina Children's Hospital and UMC Utrecht Utrecht The Netherlands 8 Mouse Clinic for Cancer and Aging (MCCA) Preclinical Intervention Unit The Netherlands Cancer Institute Amsterdam The Netherlands 4 The Netherlands Cancer Institute Amsterdam The Netherlands 3 St. Antonius Hospital Nieuwegein Nieuwegein The Netherlands 7 UMC Utrecht Utrecht The Netherlands 10 Princess Máxima Center for Pediatric Oncology Utrecht The Netherlands 1 Oncode Institute Hubrecht Institute‐KNAW and UMC Utrecht Utrecht The Netherlands 9 Hubrecht Organoid Technology Utrecht The Netherlands 5 FOM Institute AMOLF Amsterdam The Netherlands 6 Maastricht University Maastricht The Netherlands |
| AuthorAffiliation_xml | – name: 2 Wilhelmina Children's Hospital and UMC Utrecht Utrecht The Netherlands – name: 4 The Netherlands Cancer Institute Amsterdam The Netherlands – name: 6 Maastricht University Maastricht The Netherlands – name: 8 Mouse Clinic for Cancer and Aging (MCCA) Preclinical Intervention Unit The Netherlands Cancer Institute Amsterdam The Netherlands – name: 9 Hubrecht Organoid Technology Utrecht The Netherlands – name: 1 Oncode Institute Hubrecht Institute‐KNAW and UMC Utrecht Utrecht The Netherlands – name: 7 UMC Utrecht Utrecht The Netherlands – name: 10 Princess Máxima Center for Pediatric Oncology Utrecht The Netherlands – name: 3 St. Antonius Hospital Nieuwegein Nieuwegein The Netherlands – name: 5 FOM Institute AMOLF Amsterdam The Netherlands |
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| Keywords | lung cancer 3D culture respiratory syncytial virus cystic fibrosis airway organoids |
| Language | English |
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| PublicationTitleAbbrev | EMBO J |
| PublicationTitleAlternate | EMBO J |
| PublicationYear | 2019 |
| Publisher | Nature Publishing Group UK Springer Nature B.V John Wiley and Sons Inc |
| Publisher_xml | – name: Nature Publishing Group UK – name: Springer Nature B.V – name: John Wiley and Sons Inc |
| References | Hrycaj, Dye, Baker, Larsen, Burke, Spence, Wellik (CR28) 2015; 12 Guerrero‐Plata, Casola, Suarez, Yu, Spetch, Peeples, Garofalo (CR23) 2006; 34 Mou, Vinarsky, Tata, Brazauskas, Choi, Crooke, Zhang, Solomon, Turner, Bihler, Harrington, Lapey, Channick, Keyes, Freund, Artandi, Mense, Rowe, Engelhardt, Hsu (CR49) 2016; 19 Li, Durbin (CR42) 2009; 25 Vlachogiannis, Hedayat, Vatsiou, Jamin, Fernandez‐Mateos, Khan, Lampis, Eason, Huntingford, Burke, Rata, Koh, Tunariu, Collins, Hulkki‐Wilson, Ragulan, Spiteri, Moorcraft, Chau, Rao (CR72) 2018; 359 CR31 Montoro, Haber, Biton, Vinarsky, Lin, Birket, Yuan, Chen, Leung, Villoria, Rogel, Burgin, Tsankov, Waghray, Slyper, Waldman, Nguyen, Dionne, Rozenblatt‐Rosen, Tata (CR47) 2018; 560 Faas, Avramut, van den Berg, Mommaas, Koster, Ravelli (CR19) 2012; 198 Barkauskas, Chung, Fioret, Gao, Katsura, Hogan (CR3) 2017; 144 Nikolic, Caritg, Jeng, Johnson, Sun, Howell, Brady, Laresgoiti, Allen, Butler, Zilbauer, Giangreco, Rawlins (CR53) 2017; 6 Treutlein, Brownfield, Wu, Neff, Mantalas, Espinoza, Desai, Krasnow, Quake (CR70) 2014; 509 Hild, Jaffe (CR27) 2016; 37 Konishi, Gotoh, Tateishi, Yamamoto, Korogi, Nagasaki, Matsumoto, Muro, Hirai, Ito, Tsukita, Mishima (CR37) 2016; 6 Heo, Dutta, Schaefer, Iakobachvili, Artegiani, Sachs, Boonekamp, Bowden, Hendrickx, Willems, Peters, Riggs, O'Connor, Clevers (CR26) 2018; 3 Lindskog, Fagerberg, Hallstrom, Edlund, Hellwig, Rahnenfuhrer, Kampf, Uhlen, Ponten, Micke (CR44) 2014; 28 CR8 Ferkol, Schraufnagel (CR20) 2014; 11 Weeber, Ooft, Dijkstra, Voest (CR73) 2017; 24 Benali, Tournier, Chevillard, Zahm, Klossek, Hinnrasky, Gaillard, Maquart, Puchelle (CR4) 1993; 264 van de Wetering, Francies, Francis, Bounova, Iorio, Pronk, van Houdt, van Gorp, Taylor‐Weiner, Kester, McLaren‐Douglas, Blokker, Jaksani, Bartfeld, Volckman, van Sluis, Li, Seepo, Sekhar Pedamallu, Cibulskis (CR74) 2015; 161 Balasooriya, Goschorska, Piddini, Rawlins (CR2) 2017; 144 Neuberger, Burton, Clark, Van Goor (CR52) 2011; 741 Huang, Incognito, Cheng, Ulbrandt, Wu (CR29) 2010; 84 Rock, Onaitis, Rawlins, Lu, Clark, Xue, Randell, Hogan (CR58) 2009; 106 Deben, Deschoolmeester, Lardon, Rolfo, Pauwels (CR11) 2016; 99 DePristo, Banks, Poplin, Garimella, Maguire, Hartl, Philippakis, del Angel, Rivas, Hanna, McKenna, Fennell, Kernytsky, Sivachenko, Cibulskis, Gabriel, Altshuler, Daly (CR14) 2011; 43 CR40 van der Sanden, Sachs, Koekkoek, Koen, Pajkrt, Clevers, Wolthers (CR59) 2018; 7 Dekkers, Berkers, Kruisselbrink, Vonk, de Jonge, Janssens, Bronsveld, van de Graaf, Nieuwenhuis, Houwen, Vleggaar, Escher, de Rijke, Majoor, Heijerman, de Winter‐de Groot, Clevers, van der Ent, Beekman (CR13) 2016; 8 Dijkstra, Cattaneo, Weeber, Chalabi, van de Haar, Fanchi, Slagter, van der Velden, Kaing, Kelderman, van Rooij, van Leerdam, Depla, Smit, Hartemink, Groot, Wolkers, Sachs, Snaebjornsson, Monkhorst (CR15) 2018; 174 Liesman, Buchholz, Luongo, Yang, Proia, DeVincenzo, Collins, Pickles (CR43) 2014; 124 Ratjen, Bell, Rowe, Goss, Quittner, Bush (CR57) 2015; 1 Boj, Hwang, Baker, Chio, Engle, Corbo, Jager, Ponz‐Sarvise, Tiriac, Spector, Gracanin, Oni, Yu, van Boxtel, Huch, Rivera, Wilson, Feigin, Ohlund, Handly‐Santana (CR5) 2015; 160 Kim, Lee, Li, Park, Park, Lee, Kim, Shin, Choi (CR36) 2011; 6 Hackett, Butler, Shaykhiev, Salit, Omberg, Rodriguez‐Flores, Mezey, Strulovici‐Barel, Wang, Didon, Crystal (CR24) 2012; 13 Hallak, Collins, Knudson, Peeples (CR25) 2000; 271 Zhou, Li, Sachs, Chiu, Wong, Chu, Poon, Wang, Zhao, Wen, Song, Yuan, Wong, Chan, To, Chen, Clevers, Yuen (CR77) 2018; 115 McCauley, Hawkins, Serra, Thomas, Jacob, Kotton (CR46) 2017; 20 Hui, Ching, Chan, Nicholls, Sachs, Clevers, Peiris, Chan (CR33) 2018; 6 Namkung, Yao, Finkbeiner, Verkman (CR51) 2011; 25 Wong, Bear, Chin, Pasceri, Thompson, Huan, Ratjen, Ellis, Rossant (CR75) 2012; 30 Tan, Choi, Sicard, Tschumperlin (CR68) 2017; 113 Nair, Nokes, Gessner, Dherani, Madhi, Singleton, O'Brien, Roca, Wright, Bruce, Chandran, Theodoratou, Sutanto, Sedyaningsih, Ngama, Munywoki, Kartasasmita, Simoes, Rudan, Weber (CR50) 2010; 375 Dvorak, Tilley, Shaykhiev, Wang, Crystal (CR17) 2011; 44 Chen, Huang, de Carvalho, Ho, Islam, Volpi, Notarangelo, Ciancanelli, Casanova, Bhattacharya, Liang, Palermo, Porotto, Moscona, Snoeck (CR10) 2017; 19 Dye, Hill, Ferguson, Tsai, Nagy, Dyal, Wells, Mayhew, Nattiv, Klein, White, Deutsch, Spence (CR18) 2015; 4 Noordhoek, Gulmans, van der Ent, Beekman (CR54) 2016; 22 Plasschaert, Zilionis, Choo‐Wing, Savova, Knehr, Roma, Klein, Jaffe (CR56) 2018; 560 Yonker, Mou, Chu, Pazos, Leung, Cui, Ryu, Hibbler, Eaton, Ford, Falck, Kinane, Tearney, Rajagopal, Hurley (CR76) 2017; 7 Persson, Jaffe, Fearns, Danahay (CR55) 2014; 9 Sato, Stange, Ferrante, Vries, Van Es, Van den Brink, Van Houdt, Pronk, Van Gorp, Siersema, Clevers (CR60) 2011; 141 Dekkers, Wiegerinck, de Jonge, Bronsveld, Janssens, de Winter‐de Groot, Brandsma, de Jong, Bijvelds, Scholte, Nieuwenhuis, van den Brink, Clevers, van der Ent, Middendorp, Beekman (CR12) 2013; 19 Mootha, Lindgren, Eriksson, Subramanian, Sihag, Lehar, Puigserver, Carlsson, Ridderstrale, Laurila, Houstis, Daly, Patterson, Mesirov, Golub, Tamayo, Spiegelman, Lander, Hirschhorn, Altshuler (CR48) 2003; 34 Tadokoro, Gao, Hong, Hotten, Hogan (CR67) 2016; 143 Sherry, Ward, Kholodov, Baker, Phan, Smigielski, Sirotkin (CR62) 2001; 29 Teng, Collins (CR69) 1999; 73 Subramanian, Tamayo, Mootha, Mukherjee, Ebert, Gillette, Paulovich, Pomeroy, Golub, Lander, Mesirov (CR65) 2005; 102 Vassilev, Vu, Graves, Carvajal, Podlaski, Filipovic, Kong, Kammlott, Lukacs, Klein, Fotouhi, Liu (CR71) 2004; 303 de Jager, te Velthuis, Prakken, Kuis, Rijkers (CR34) 2003; 10 Sondo, Caci, Galietta (CR63) 2014; 52 Sosnay, Siklosi, Van Goor, Kaniecki, Yu, Sharma, Ramalho, Amaral, Dorfman, Zielenski, Masica, Karchin, Millen, Thomas, Patrinos, Corey, Lewis, Rommens, Castellani, Penland (CR64) 2013; 45 Fulcher, Gabriel, Burns, Yankaskas, Randell (CR21) 2005; 107 Geerdink, Pillay, Meyaard, Bont (CR22) 2015; 136 Lo, Brazas, Holtzman (CR45) 2005; 79 Boomsma, Wijmenga, Slagboom, Swertz, Karssen, Abdellaoui, Ye, Guryev, Vermaat, van Dijk, Francioli, Hottenga, Laros, Li, Li, Cao, Chen, Du, Li, Cao (CR6) 2014; 22 Koo, Stange, Sato, Karthaus, Farin, Huch, van Es, Clevers (CR38) 2011; 9 Huch, Gehart, van Boxtel, Hamer, Blokzijl, Verstegen, Ellis, van Wenum, Fuchs, de Ligt, van de Wetering, Sasaki, Boers, Kemperman, de Jonge, Ijzermans, Nieuwenhuis, Hoekstra, Strom, Vries (CR32) 2015; 160 Amatngalim, van Wijck, de Mooij‐Eijk, Verhoosel, Harder, Lekkerkerker, Janssen, Hiemstra (CR1) 2015; 194 Huang, Islam, O'Neill, Hu, Yang, Chen, Mumau, Green, Vunjak‐Novakovic, Bhattacharya, Snoeck (CR30) 2014; 32 Karthaus, Iaquinta, Drost, Gracanin, van Boxtel, Wongvipat, Dowling, Gao, Begthel, Sachs, Vries, Cuppen, Chen, Sawyers, Clevers (CR35) 2014; 159 Borchers, Chang, Gershwin, Gershwin (CR7) 2013; 45 Chen, Fillmore, Hammerman, Kim, Wong (CR9) 2014; 14 Tadokoro, Wang, Barak, Bai, Randell, Hogan (CR66) 2014; 111 Kuk, Taylor‐Cousar (CR39) 2015; 9 Lek, Karczewski, Minikel, Samocha, Banks, Fennell, O'Donnell‐Luria, Ware, Hill, Cummings, Tukiainen, Birnbaum, Kosmicki, Duncan, Estrada, Zhao, Zou, Pierce‐Hoffman, Berghout, Cooper (CR41) 2016; 536 2018; 560 2017; 6 2017; 7 2006; 34 2016; 143 2014; 28 2012; 13 2017; 113 2016; 37 2014; 22 2003; 10 2013; 19 2018; 7 2018; 174 2018; 6 2018; 3 2015; 136 2005; 102 2005; 107 2014; 14 2011; 25 2014; 9 2014; 52 2014; 11 2005; 79 2014; 124 2015; 12 2015; 1 2015; 160 2009; 25 2017; 20 2015; 161 2004; 303 2015; 4 2016; 19 2011 2013; 45 2017; 24 1998 2000; 271 2001; 29 2015; 9 2014; 111 2011; 6 1993; 264 2010; 84 2014; 159 2012; 30 2016; 99 2011; 9 2003; 34 2016; 6 2011; 741 2015; 194 2012; 198 2018; 359 2014; 509 2016; 536 2018; 115 2010; 375 2011; 44 2011; 43 2017 2017; 19 1999; 73 2017; 144 2011; 141 2016; 8 2014; 32 2016; 22 2009; 106 30718273 - EMBO J. 2019 Feb 15;38(4):e101526. doi: 10.15252/embj.2019101526 |
| References_xml | – volume: 303 start-page: 844 year: 2004 end-page: 848 ident: CR71 article-title: activation of the p53 pathway by small‐molecule antagonists of MDM2 publication-title: Science – volume: 111 start-page: E3641 year: 2014 end-page: E3649 ident: CR66 article-title: IL‐6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells publication-title: Proc Natl Acad Sci USA – volume: 73 start-page: 466 year: 1999 end-page: 473 ident: CR69 article-title: Altered growth characteristics of recombinant respiratory syncytial viruses which do not produce NS2 protein publication-title: J Virol – volume: 1 start-page: 15010 year: 2015 ident: CR57 article-title: Cystic fibrosis publication-title: Nat Rev Dis Primers – volume: 22 start-page: 610 year: 2016 end-page: 616 ident: CR54 article-title: Intestinal organoids and personalized medicine in cystic fibrosis: a successful patient‐oriented research collaboration publication-title: Curr Opin Pulm Med – volume: 174 start-page: 1586 year: 2018 end-page: 1598 ident: CR15 article-title: Facilitating individualized T cell therapy by co‐culture of peripheral blood lymphocytes and tumor organoids publication-title: Cell – volume: 84 start-page: 8132 year: 2010 end-page: 8140 ident: CR29 article-title: Respiratory syncytial virus‐neutralizing monoclonal antibodies motavizumab and palivizumab inhibit fusion publication-title: J Virol – volume: 359 start-page: 920 year: 2018 end-page: 926 ident: CR72 article-title: Patient‐derived organoids model treatment response of metastatic gastrointestinal cancers publication-title: Science – volume: 560 start-page: 377 year: 2018 end-page: 381 ident: CR56 article-title: A single‐cell atlas of the airway epithelium reveals the CFTR‐rich pulmonary ionocyte publication-title: Nature – volume: 45 start-page: 331 year: 2013 end-page: 379 ident: CR7 article-title: Respiratory syncytial virus–a comprehensive review publication-title: Clin Rev Allergy Immunol – volume: 7 start-page: 84 year: 2018 ident: CR59 article-title: Enterovirus 71 infection of human airway organoids reveals VP1‐145 as a viral infectivity determinant publication-title: Emerg Microbes Infect – volume: 28 start-page: 5184 year: 2014 end-page: 5196 ident: CR44 article-title: The lung‐specific proteome defined by integration of transcriptomics and antibody‐based profiling publication-title: FASEB J – volume: 102 start-page: 15545 year: 2005 end-page: 15550 ident: CR65 article-title: Gene set enrichment analysis: a knowledge‐based approach for interpreting genome‐wide expression profiles publication-title: Proc Natl Acad Sci USA – volume: 113 start-page: 118 year: 2017 end-page: 132 ident: CR68 article-title: Human airway organoid engineering as a step toward lung regeneration and disease modeling publication-title: Biomaterials – volume: 3 start-page: 814 year: 2018 end-page: 823 ident: CR26 article-title: Modelling Cryptosporidium infection in human small intestinal and lung organoids publication-title: Nat Microbiol – volume: 7 start-page: 8182 year: 2017 ident: CR76 article-title: Development of a primary human co‐culture model of inflamed airway mucosa publication-title: Sci Rep – ident: CR8 – volume: 14 start-page: 535 year: 2014 end-page: 546 ident: CR9 article-title: Non‐small‐cell lung cancers: a heterogeneous set of diseases publication-title: Nat Rev Cancer – volume: 194 start-page: 3340 year: 2015 end-page: 3350 ident: CR1 article-title: Basal cells contribute to innate immunity of the airway epithelium through production of the antimicrobial protein RNase 7 publication-title: J Immunol – volume: 43 start-page: 491 year: 2011 end-page: 498 ident: CR14 article-title: A framework for variation discovery and genotyping using next‐generation DNA sequencing data publication-title: Nat Genet – volume: 4 start-page: e05098 year: 2015 ident: CR18 article-title: generation of human pluripotent stem cell derived lung organoids publication-title: Elife – volume: 160 start-page: 299 year: 2015 end-page: 312 ident: CR32 article-title: Long‐term culture of genome‐stable bipotent stem cells from adult human liver publication-title: Cell – volume: 99 start-page: 63 year: 2016 end-page: 73 ident: CR11 article-title: TP53 and MDM2 genetic alterations in non‐small cell lung cancer: evaluating their prognostic and predictive value publication-title: Crit Rev Oncol Hematol – volume: 34 start-page: 267 year: 2003 end-page: 273 ident: CR48 article-title: PGC‐1alpha‐responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes publication-title: Nat Genet – volume: 144 start-page: 1600 year: 2017 end-page: 1606 ident: CR2 article-title: FGFR2 is required for airway basal cell self‐renewal and terminal differentiation publication-title: Development – volume: 141 start-page: 1762 year: 2011 end-page: 1772 ident: CR60 article-title: Long‐term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium publication-title: Gastroenterology – volume: 143 start-page: 764 year: 2016 end-page: 773 ident: CR67 article-title: BMP signaling and cellular dynamics during regeneration of airway epithelium from basal progenitors publication-title: Development – volume: 10 start-page: 133 year: 2003 end-page: 139 ident: CR34 article-title: Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells publication-title: Clin Diagn Lab Immunol – volume: 11 start-page: 404 year: 2014 end-page: 406 ident: CR20 article-title: The global burden of respiratory disease publication-title: Ann Am Thorac Soc – volume: 136 start-page: 838 year: 2015 end-page: 847 ident: CR22 article-title: Neutrophils in respiratory syncytial virus infection: a target for asthma prevention publication-title: J Allergy Clin Immunol – volume: 6 start-page: 846 year: 2018 end-page: 854 ident: CR33 article-title: Tropism, replication competence, and innate immune responses of influenza virus: an analysis of human airway organoids and ex‐vivo bronchus cultures publication-title: Lancet Respir Med – volume: 264 start-page: L183 year: 1993 end-page: L192 ident: CR4 article-title: Tubule formation by human surface respiratory epithelial cells cultured in a three‐dimensional collagen lattice publication-title: Am J Physiol – volume: 19 start-page: 217 year: 2016 end-page: 231 ident: CR49 article-title: Dual SMAD signaling inhibition enables long‐term expansion of diverse epithelial basal cells publication-title: Cell Stem Cell – volume: 45 start-page: 1160 year: 2013 end-page: 1167 ident: CR64 article-title: Defining the disease liability of variants in the cystic fibrosis transmembrane conductance regulator gene publication-title: Nat Genet – volume: 25 start-page: 4048 year: 2011 end-page: 4062 ident: CR51 article-title: Small‐molecule activators of TMEM16A, a calcium‐activated chloride channel, stimulate epithelial chloride secretion and intestinal contraction publication-title: FASEB J – volume: 24 start-page: 1092 year: 2017 end-page: 1100 ident: CR73 article-title: Tumor organoids as a pre‐clinical cancer model for drug discovery publication-title: Cell Chem Biol – volume: 198 start-page: 457 year: 2012 end-page: 469 ident: CR19 article-title: Virtual nanoscopy: generation of ultra‐large high resolution electron microscopy maps publication-title: J Cell Biol – volume: 107 start-page: 183 year: 2005 end-page: 206 ident: CR21 article-title: Well‐differentiated human airway epithelial cell cultures publication-title: Methods Mol Med – volume: 741 start-page: 39 year: 2011 end-page: 54 ident: CR52 article-title: Use of primary cultures of human bronchial epithelial cells isolated from cystic fibrosis patients for the pre‐clinical testing of CFTR modulators publication-title: Methods Mol Biol – volume: 115 start-page: 6822 year: 2018 end-page: 6827 ident: CR77 article-title: Differentiated human airway organoids to assess infectivity of emerging influenza virus publication-title: Proc Natl Acad Sci USA – volume: 19 start-page: 939 year: 2013 end-page: 945 ident: CR12 article-title: A functional CFTR assay using primary cystic fibrosis intestinal organoids publication-title: Nat Med – volume: 30 start-page: 876 year: 2012 end-page: 882 ident: CR75 article-title: Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein publication-title: Nat Biotechnol – volume: 6 start-page: e18556 year: 2011 ident: CR36 article-title: High cleavage efficiency of a 2A peptide derived from porcine teschovirus‐1 in human cell lines, zebrafish and mice publication-title: PLoS One – volume: 375 start-page: 1545 year: 2010 end-page: 1555 ident: CR50 article-title: Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta‐analysis publication-title: Lancet – volume: 160 start-page: 324 year: 2015 end-page: 338 ident: CR5 article-title: Organoid models of human and mouse ductal pancreatic cancer publication-title: Cell – volume: 106 start-page: 12771 year: 2009 end-page: 12775 ident: CR58 article-title: Basal cells as stem cells of the mouse trachea and human airway epithelium publication-title: Proc Natl Acad Sci USA – volume: 161 start-page: 933 year: 2015 end-page: 945 ident: CR74 article-title: Prospective derivation of a living organoid biobank of colorectal cancer patients publication-title: Cell – volume: 509 start-page: 371 year: 2014 end-page: 375 ident: CR70 article-title: Reconstructing lineage hierarchies of the distal lung epithelium using single‐cell RNA‐seq publication-title: Nature – volume: 6 start-page: e26575 year: 2017 ident: CR53 article-title: Human embryonic lung epithelial tips are multipotent progenitors that can be expanded as long‐term self‐renewing organoids publication-title: Elife – volume: 34 start-page: 320 year: 2006 end-page: 329 ident: CR23 article-title: Differential response of dendritic cells to human metapneumovirus and respiratory syncytial virus publication-title: Am J Respir Cell Mol Biol – volume: 22 start-page: 221 year: 2014 end-page: 227 ident: CR6 article-title: The Genome of the Netherlands: design, and project goals publication-title: Eur J Hum Genet – volume: 144 start-page: 986 year: 2017 end-page: 997 ident: CR3 article-title: Lung organoids: current uses and future promise publication-title: Development – volume: 79 start-page: 9315 year: 2005 end-page: 9319 ident: CR45 article-title: Respiratory syncytial virus nonstructural proteins NS1 and NS2 mediate inhibition of Stat2 expression and alpha/beta interferon responsiveness publication-title: J Virol – ident: CR40 – volume: 13 start-page: 82 year: 2012 ident: CR24 article-title: RNA‐Seq quantification of the human small airway epithelium transcriptome publication-title: BMC Genom – volume: 44 start-page: 465 year: 2011 end-page: 473 ident: CR17 article-title: Do airway epithelium air‐liquid cultures represent the airway epithelium transcriptome? publication-title: Am J Respir Cell Mol Biol – volume: 9 start-page: e102368 year: 2014 ident: CR55 article-title: Respiratory syncytial virus can infect basal cells and alter human airway epithelial differentiation publication-title: PLoS One – volume: 536 start-page: 285 year: 2016 end-page: 291 ident: CR41 article-title: Analysis of protein‐coding genetic variation in 60,706 humans publication-title: Nature – volume: 8 start-page: 344ra84 year: 2016 ident: CR13 article-title: Characterizing responses to CFTR‐modulating drugs using rectal organoids derived from subjects with cystic fibrosis publication-title: Sci Transl Med – volume: 6 start-page: 18 year: 2016 end-page: 25 ident: CR37 article-title: Directed induction of functional multi‐ciliated cells in proximal airway epithelial spheroids from human pluripotent stem cells publication-title: Stem Cell Reports – volume: 9 start-page: 313 year: 2015 end-page: 326 ident: CR39 article-title: Lumacaftor and ivacaftor in the management of patients with cystic fibrosis: current evidence and future prospects publication-title: Ther Adv Respir Dis – volume: 32 start-page: 84 year: 2014 end-page: 91 ident: CR30 article-title: Efficient generation of lung and airway epithelial cells from human pluripotent stem cells publication-title: Nat Biotechnol – volume: 20 start-page: 844 year: 2017 end-page: 857 ident: CR46 article-title: Efficient derivation of functional human airway epithelium from pluripotent stem cells via temporal regulation of Wnt signaling publication-title: Cell Stem Cell – volume: 12 start-page: 903 year: 2015 end-page: 912 ident: CR28 article-title: Hox5 genes regulate the Wnt2/2b‐Bmp4‐signaling axis during lung development publication-title: Cell Rep – ident: CR31 – volume: 9 start-page: 81 year: 2011 end-page: 83 ident: CR38 article-title: Controlled gene expression in primary Lgr5 organoid cultures publication-title: Nat Methods – volume: 124 start-page: 2219 year: 2014 end-page: 2233 ident: CR43 article-title: RSV‐encoded NS2 promotes epithelial cell shedding and distal airway obstruction publication-title: J Clin Invest – volume: 19 start-page: 542 year: 2017 end-page: 549 ident: CR10 article-title: A three‐dimensional model of human lung development and disease from pluripotent stem cells publication-title: Nat Cell Biol – volume: 25 start-page: 1754 year: 2009 end-page: 1760 ident: CR42 article-title: Fast and accurate short read alignment with Burrows‐Wheeler transform publication-title: Bioinformatics – volume: 159 start-page: 163 year: 2014 end-page: 175 ident: CR35 article-title: Identification of multipotent luminal progenitor cells in human prostate organoid cultures publication-title: Cell – volume: 52 start-page: 73 year: 2014 end-page: 76 ident: CR63 article-title: The TMEM16A chloride channel as an alternative therapeutic target in cystic fibrosis publication-title: Int J Biochem Cell Biol – volume: 271 start-page: 264 year: 2000 end-page: 275 ident: CR25 article-title: Iduronic acid‐containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection publication-title: Virology – volume: 29 start-page: 308 year: 2001 end-page: 311 ident: CR62 article-title: dbSNP: the NCBI database of genetic variation publication-title: Nucleic Acids Res – volume: 37 start-page: IE 9 1 year: 2016 end-page: IE 9 15 ident: CR27 article-title: Production of 3‐D airway organoids from primary human airway basal cells and their use in high‐throughput screening publication-title: Curr Protoc Stem Cell Biol – volume: 560 start-page: 319 year: 2018 end-page: 324 ident: CR47 article-title: A revised airway epithelial hierarchy includes CFTR‐expressing ionocytes publication-title: Nature – volume: 32 start-page: 84 year: 2014 end-page: 91 article-title: Efficient generation of lung and airway epithelial cells from human pluripotent stem cells publication-title: Nat Biotechnol – year: 2011 – volume: 6 start-page: e18556 year: 2011 article-title: High cleavage efficiency of a 2A peptide derived from porcine teschovirus‐1 in human cell lines, zebrafish and mice publication-title: PLoS One – volume: 79 start-page: 9315 year: 2005 end-page: 9319 article-title: Respiratory syncytial virus nonstructural proteins NS1 and NS2 mediate inhibition of Stat2 expression and alpha/beta interferon responsiveness publication-title: J Virol – volume: 141 start-page: 1762 year: 2011 end-page: 1772 article-title: Long‐term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium publication-title: Gastroenterology – volume: 194 start-page: 3340 year: 2015 end-page: 3350 article-title: Basal cells contribute to innate immunity of the airway epithelium through production of the antimicrobial protein RNase 7 publication-title: J Immunol – volume: 84 start-page: 8132 year: 2010 end-page: 8140 article-title: Respiratory syncytial virus‐neutralizing monoclonal antibodies motavizumab and palivizumab inhibit fusion publication-title: J Virol – volume: 22 start-page: 610 year: 2016 end-page: 616 article-title: Intestinal organoids and personalized medicine in cystic fibrosis: a successful patient‐oriented research collaboration publication-title: Curr Opin Pulm Med – volume: 43 start-page: 491 year: 2011 end-page: 498 article-title: A framework for variation discovery and genotyping using next‐generation DNA sequencing data publication-title: Nat Genet – volume: 6 start-page: e26575 year: 2017 article-title: Human embryonic lung epithelial tips are multipotent progenitors that can be expanded as long‐term self‐renewing organoids publication-title: Elife – volume: 113 start-page: 118 year: 2017 end-page: 132 article-title: Human airway organoid engineering as a step toward lung regeneration and disease modeling publication-title: Biomaterials – volume: 22 start-page: 221 year: 2014 end-page: 227 article-title: The Genome of the Netherlands: design, and project goals publication-title: Eur J Hum Genet – volume: 264 start-page: L183 year: 1993 end-page: L192 article-title: Tubule formation by human surface respiratory epithelial cells cultured in a three‐dimensional collagen lattice publication-title: Am J Physiol – volume: 107 start-page: 183 year: 2005 end-page: 206 article-title: Well‐differentiated human airway epithelial cell cultures publication-title: Methods Mol Med – volume: 34 start-page: 267 year: 2003 end-page: 273 article-title: PGC‐1alpha‐responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes publication-title: Nat Genet – volume: 52 start-page: 73 year: 2014 end-page: 76 article-title: The TMEM16A chloride channel as an alternative therapeutic target in cystic fibrosis publication-title: Int J Biochem Cell Biol – volume: 198 start-page: 457 year: 2012 end-page: 469 article-title: Virtual nanoscopy: generation of ultra‐large high resolution electron microscopy maps publication-title: J Cell Biol – year: 1998 – volume: 136 start-page: 838 year: 2015 end-page: 847 article-title: Neutrophils in respiratory syncytial virus infection: a target for asthma prevention publication-title: J Allergy Clin Immunol – volume: 741 start-page: 39 year: 2011 end-page: 54 article-title: Use of primary cultures of human bronchial epithelial cells isolated from cystic fibrosis patients for the pre‐clinical testing of CFTR modulators publication-title: Methods Mol Biol – volume: 144 start-page: 986 year: 2017 end-page: 997 article-title: Lung organoids: current uses and future promise publication-title: Development – volume: 14 start-page: 535 year: 2014 end-page: 546 article-title: Non‐small‐cell lung cancers: a heterogeneous set of diseases publication-title: Nat Rev Cancer – volume: 12 start-page: 903 year: 2015 end-page: 912 article-title: Hox5 genes regulate the Wnt2/2b‐Bmp4‐signaling axis during lung development publication-title: Cell Rep – volume: 303 start-page: 844 year: 2004 end-page: 848 article-title: activation of the p53 pathway by small‐molecule antagonists of MDM2 publication-title: Science – volume: 159 start-page: 163 year: 2014 end-page: 175 article-title: Identification of multipotent luminal progenitor cells in human prostate organoid cultures publication-title: Cell – volume: 560 start-page: 319 year: 2018 end-page: 324 article-title: A revised airway epithelial hierarchy includes CFTR‐expressing ionocytes publication-title: Nature – volume: 144 start-page: 1600 year: 2017 end-page: 1606 article-title: FGFR2 is required for airway basal cell self‐renewal and terminal differentiation publication-title: Development – volume: 375 start-page: 1545 year: 2010 end-page: 1555 article-title: Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta‐analysis publication-title: Lancet – volume: 29 start-page: 308 year: 2001 end-page: 311 article-title: dbSNP: the NCBI database of genetic variation publication-title: Nucleic Acids Res – volume: 44 start-page: 465 year: 2011 end-page: 473 article-title: Do airway epithelium air‐liquid cultures represent the airway epithelium transcriptome? publication-title: Am J Respir Cell Mol Biol – volume: 45 start-page: 331 year: 2013 end-page: 379 article-title: Respiratory syncytial virus–a comprehensive review publication-title: Clin Rev Allergy Immunol – volume: 99 start-page: 63 year: 2016 end-page: 73 article-title: TP53 and MDM2 genetic alterations in non‐small cell lung cancer: evaluating their prognostic and predictive value publication-title: Crit Rev Oncol Hematol – volume: 9 start-page: 313 year: 2015 end-page: 326 article-title: Lumacaftor and ivacaftor in the management of patients with cystic fibrosis: current evidence and future prospects publication-title: Ther Adv Respir Dis – volume: 271 start-page: 264 year: 2000 end-page: 275 article-title: Iduronic acid‐containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection publication-title: Virology – volume: 102 start-page: 15545 year: 2005 end-page: 15550 article-title: Gene set enrichment analysis: a knowledge‐based approach for interpreting genome‐wide expression profiles publication-title: Proc Natl Acad Sci USA – volume: 9 start-page: 81 year: 2011 end-page: 83 article-title: Controlled gene expression in primary Lgr5 organoid cultures publication-title: Nat Methods – volume: 30 start-page: 876 year: 2012 end-page: 882 article-title: Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein publication-title: Nat Biotechnol – volume: 3 start-page: 814 year: 2018 end-page: 823 article-title: Modelling Cryptosporidium infection in human small intestinal and lung organoids publication-title: Nat Microbiol – volume: 19 start-page: 217 year: 2016 end-page: 231 article-title: Dual SMAD signaling inhibition enables long‐term expansion of diverse epithelial basal cells publication-title: Cell Stem Cell – volume: 1 start-page: 15010 year: 2015 article-title: Cystic fibrosis publication-title: Nat Rev Dis Primers – volume: 143 start-page: 764 year: 2016 end-page: 773 article-title: BMP signaling and cellular dynamics during regeneration of airway epithelium from basal progenitors publication-title: Development – volume: 13 start-page: 82 year: 2012 article-title: RNA‐Seq quantification of the human small airway epithelium transcriptome publication-title: BMC Genom – volume: 160 start-page: 324 year: 2015 end-page: 338 article-title: Organoid models of human and mouse ductal pancreatic cancer publication-title: Cell – volume: 10 start-page: 133 year: 2003 end-page: 139 article-title: Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells publication-title: Clin Diagn Lab Immunol – volume: 24 start-page: 1092 year: 2017 end-page: 1100 article-title: Tumor organoids as a pre‐clinical cancer model for drug discovery publication-title: Cell Chem Biol – volume: 19 start-page: 542 year: 2017 end-page: 549 article-title: A three‐dimensional model of human lung development and disease from pluripotent stem cells publication-title: Nat Cell Biol – volume: 161 start-page: 933 year: 2015 end-page: 945 article-title: Prospective derivation of a living organoid biobank of colorectal cancer patients publication-title: Cell – volume: 34 start-page: 320 year: 2006 end-page: 329 article-title: Differential response of dendritic cells to human metapneumovirus and respiratory syncytial virus publication-title: Am J Respir Cell Mol Biol – volume: 509 start-page: 371 year: 2014 end-page: 375 article-title: Reconstructing lineage hierarchies of the distal lung epithelium using single‐cell RNA‐seq publication-title: Nature – volume: 7 start-page: 8182 year: 2017 article-title: Development of a primary human co‐culture model of inflamed airway mucosa publication-title: Sci Rep – volume: 37 start-page: IE 9 1 year: 2016 end-page: IE 9 15 article-title: Production of 3‐D airway organoids from primary human airway basal cells and their use in high‐throughput screening publication-title: Curr Protoc Stem Cell Biol – volume: 25 start-page: 1754 year: 2009 end-page: 1760 article-title: Fast and accurate short read alignment with Burrows‐Wheeler transform publication-title: Bioinformatics – volume: 73 start-page: 466 year: 1999 end-page: 473 article-title: Altered growth characteristics of recombinant respiratory syncytial viruses which do not produce NS2 protein publication-title: J Virol – volume: 160 start-page: 299 year: 2015 end-page: 312 article-title: Long‐term culture of genome‐stable bipotent stem cells from adult human liver publication-title: Cell – volume: 174 start-page: 1586 year: 2018 end-page: 1598 article-title: Facilitating individualized T cell therapy by co‐culture of peripheral blood lymphocytes and tumor organoids publication-title: Cell – volume: 115 start-page: 6822 year: 2018 end-page: 6827 article-title: Differentiated human airway organoids to assess infectivity of emerging influenza virus publication-title: Proc Natl Acad Sci USA – volume: 28 start-page: 5184 year: 2014 end-page: 5196 article-title: The lung‐specific proteome defined by integration of transcriptomics and antibody‐based profiling publication-title: FASEB J – volume: 7 start-page: 84 year: 2018 article-title: Enterovirus 71 infection of human airway organoids reveals VP1‐145 as a viral infectivity determinant publication-title: Emerg Microbes Infect – volume: 6 start-page: 18 year: 2016 end-page: 25 article-title: Directed induction of functional multi‐ciliated cells in proximal airway epithelial spheroids from human pluripotent stem cells publication-title: Stem Cell Reports – volume: 536 start-page: 285 year: 2016 end-page: 291 article-title: Analysis of protein‐coding genetic variation in 60,706 humans publication-title: Nature – volume: 111 start-page: E3641 year: 2014 end-page: E3649 article-title: IL‐6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells publication-title: Proc Natl Acad Sci USA – volume: 124 start-page: 2219 year: 2014 end-page: 2233 article-title: RSV‐encoded NS2 promotes epithelial cell shedding and distal airway obstruction publication-title: J Clin Invest – volume: 11 start-page: 404 year: 2014 end-page: 406 article-title: The global burden of respiratory disease publication-title: Ann Am Thorac Soc – volume: 560 start-page: 377 year: 2018 end-page: 381 article-title: A single‐cell atlas of the airway epithelium reveals the CFTR‐rich pulmonary ionocyte publication-title: Nature – volume: 4 start-page: e05098 year: 2015 article-title: generation of human pluripotent stem cell derived lung organoids publication-title: Elife – volume: 359 start-page: 920 year: 2018 end-page: 926 article-title: Patient‐derived organoids model treatment response of metastatic gastrointestinal cancers publication-title: Science – volume: 106 start-page: 12771 year: 2009 end-page: 12775 article-title: Basal cells as stem cells of the mouse trachea and human airway epithelium publication-title: Proc Natl Acad Sci USA – volume: 8 start-page: 344ra84 year: 2016 article-title: Characterizing responses to CFTR‐modulating drugs using rectal organoids derived from subjects with cystic fibrosis publication-title: Sci Transl Med – volume: 6 start-page: 846 year: 2018 end-page: 854 article-title: Tropism, replication competence, and innate immune responses of influenza virus: an analysis of human airway organoids and ex‐vivo bronchus cultures publication-title: Lancet Respir Med – year: 2017 – volume: 20 start-page: 844 year: 2017 end-page: 857 article-title: Efficient derivation of functional human airway epithelium from pluripotent stem cells via temporal regulation of Wnt signaling publication-title: Cell Stem Cell – volume: 25 start-page: 4048 year: 2011 end-page: 4062 article-title: Small‐molecule activators of TMEM16A, a calcium‐activated chloride channel, stimulate epithelial chloride secretion and intestinal contraction publication-title: FASEB J – volume: 9 start-page: e102368 year: 2014 article-title: Respiratory syncytial virus can infect basal cells and alter human airway epithelial differentiation publication-title: PLoS One – volume: 19 start-page: 939 year: 2013 end-page: 945 article-title: A functional CFTR assay using primary cystic fibrosis intestinal organoids publication-title: Nat Med – volume: 45 start-page: 1160 year: 2013 end-page: 1167 article-title: Defining the disease liability of variants in the cystic fibrosis transmembrane conductance regulator gene publication-title: Nat Genet – reference: 30718273 - EMBO J. 2019 Feb 15;38(4):e101526. doi: 10.15252/embj.2019101526 |
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| SubjectTerms | 3D culture airway organoids Alveoli Animals Basal cells Cancer Carcinoma, Non-Small-Cell Lung - drug therapy Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Non-Small-Cell Lung - pathology Cell culture Cells, Cultured Cystic fibrosis Cystic Fibrosis - metabolism Cystic Fibrosis - pathology Cystic Fibrosis Transmembrane Conductance Regulator - metabolism Disease Disease Models, Animal Drug screening Drug Screening Assays, Antitumor EMBO03 EMBO22 EMBO24 Epithelial Cells - metabolism Epithelial Cells - pathology Epithelium Female Hereditary diseases Histopathology Humans Leukocytes (neutrophilic) Lung cancer Lung diseases Lung Neoplasms - drug therapy Lung Neoplasms - metabolism Lung Neoplasms - pathology Male Metastases Mice Mice, Inbred NOD Mice, SCID Mucous membrane Mucus Mutation NS2 protein Organ Culture Techniques - methods Organoids Organoids - metabolism Organoids - pathology Proteins Resource Respiratory syncytial virus Respiratory Syncytial Virus Infections - pathology Respiratory Syncytial Virus Infections - virology Respiratory Syncytial Viruses - isolation & purification Respiratory System - metabolism Respiratory System - pathology Respiratory tract Respiratory tract diseases Screening Stem cells Structure-function relationships Three dimensional models Viral infections Viruses Xenograft Model Antitumor Assays |
| Title | Long‐term expanding human airway organoids for disease modeling |
| URI | https://link.springer.com/article/10.15252/embj.2018100300 https://onlinelibrary.wiley.com/doi/abs/10.15252%2Fembj.2018100300 https://www.ncbi.nlm.nih.gov/pubmed/30643021 https://www.proquest.com/docview/2262802851 https://www.proquest.com/docview/2179348792 https://pubmed.ncbi.nlm.nih.gov/PMC6376275 |
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